Effects of beetroot juice supplementation on vascular functional and structural changes in aged mice
Abstract This study investigated whether beetroot juice (BRJ) ingestion ameliorates aging‐induced functional and structural changes in vasculature. Aged mice (98–100 weeks old) were supplemented with BRJ (nitrate: 3.5 mmol/L) or drinking water for 4 weeks and compared with young mice (12–15 weeks ol...
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| Format: | Article |
| Language: | English |
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Wiley
2023-06-01
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| Series: | Physiological Reports |
| Subjects: | |
| Online Access: | https://doi.org/10.14814/phy2.15755 |
| _version_ | 1827589908887240704 |
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| author | Masashi Tawa Keisuke Nakagawa Mamoru Ohkita |
| author_facet | Masashi Tawa Keisuke Nakagawa Mamoru Ohkita |
| author_sort | Masashi Tawa |
| collection | DOAJ |
| description | Abstract This study investigated whether beetroot juice (BRJ) ingestion ameliorates aging‐induced functional and structural changes in vasculature. Aged mice (98–100 weeks old) were supplemented with BRJ (nitrate: 3.5 mmol/L) or drinking water for 4 weeks and compared with young mice (12–15 weeks old). The vasorelaxant response of isolated aortas to acetylcholine was markedly weaker in aged mice than in young mice, but the attenuated relaxation was significantly improved in BRJ‐supplemented aged mice. The acetylcholine‐induced relaxation was completely abolished by Nω‐nitro‐l‐arginine methyl ester in all groups. Additionally, the response to sodium nitroprusside was comparable among the three groups. The aortic medial thickness was significantly greater in aged mice than in young mice, and BRJ supplementation did not suppress this thickening. Plasma nitrate levels were significantly higher in BRJ‐supplemented aged mice than in non‐supplemented aged mice. Conversely, non‐supplemented aged mice had high plasma levels of thiobarbituric acid‐reactive substances, but the levels were suppressed in BRJ‐supplemented aged mice. These findings suggest that BRJ ingestion improves vascular endothelial dysfunction associated with aging, at least in part, by enhancing nitric oxide bioavailability and reducing oxidative stress. Therefore, beetroot ingestion may be a highly useful self‐medication option to prevent vascular aging. |
| first_indexed | 2024-03-09T01:08:56Z |
| format | Article |
| id | doaj.art-c1e24c723aa444d0823eb202f6a1ae15 |
| institution | Directory Open Access Journal |
| issn | 2051-817X |
| language | English |
| last_indexed | 2024-03-09T01:08:56Z |
| publishDate | 2023-06-01 |
| publisher | Wiley |
| record_format | Article |
| series | Physiological Reports |
| spelling | doaj.art-c1e24c723aa444d0823eb202f6a1ae152023-12-11T09:06:36ZengWileyPhysiological Reports2051-817X2023-06-011112n/an/a10.14814/phy2.15755Effects of beetroot juice supplementation on vascular functional and structural changes in aged miceMasashi Tawa0Keisuke Nakagawa1Mamoru Ohkita2Department of Pathological and Molecular Pharmacology, Faculty of Pharmacy Osaka Medical and Pharmaceutical University Osaka JapanDepartment of Pathological and Molecular Pharmacology, Faculty of Pharmacy Osaka Medical and Pharmaceutical University Osaka JapanDepartment of Pathological and Molecular Pharmacology, Faculty of Pharmacy Osaka Medical and Pharmaceutical University Osaka JapanAbstract This study investigated whether beetroot juice (BRJ) ingestion ameliorates aging‐induced functional and structural changes in vasculature. Aged mice (98–100 weeks old) were supplemented with BRJ (nitrate: 3.5 mmol/L) or drinking water for 4 weeks and compared with young mice (12–15 weeks old). The vasorelaxant response of isolated aortas to acetylcholine was markedly weaker in aged mice than in young mice, but the attenuated relaxation was significantly improved in BRJ‐supplemented aged mice. The acetylcholine‐induced relaxation was completely abolished by Nω‐nitro‐l‐arginine methyl ester in all groups. Additionally, the response to sodium nitroprusside was comparable among the three groups. The aortic medial thickness was significantly greater in aged mice than in young mice, and BRJ supplementation did not suppress this thickening. Plasma nitrate levels were significantly higher in BRJ‐supplemented aged mice than in non‐supplemented aged mice. Conversely, non‐supplemented aged mice had high plasma levels of thiobarbituric acid‐reactive substances, but the levels were suppressed in BRJ‐supplemented aged mice. These findings suggest that BRJ ingestion improves vascular endothelial dysfunction associated with aging, at least in part, by enhancing nitric oxide bioavailability and reducing oxidative stress. Therefore, beetroot ingestion may be a highly useful self‐medication option to prevent vascular aging.https://doi.org/10.14814/phy2.15755agingbeetrootendothelial dysfunctionmedial thickeningnitrateoxidative stress |
| spellingShingle | Masashi Tawa Keisuke Nakagawa Mamoru Ohkita Effects of beetroot juice supplementation on vascular functional and structural changes in aged mice Physiological Reports aging beetroot endothelial dysfunction medial thickening nitrate oxidative stress |
| title | Effects of beetroot juice supplementation on vascular functional and structural changes in aged mice |
| title_full | Effects of beetroot juice supplementation on vascular functional and structural changes in aged mice |
| title_fullStr | Effects of beetroot juice supplementation on vascular functional and structural changes in aged mice |
| title_full_unstemmed | Effects of beetroot juice supplementation on vascular functional and structural changes in aged mice |
| title_short | Effects of beetroot juice supplementation on vascular functional and structural changes in aged mice |
| title_sort | effects of beetroot juice supplementation on vascular functional and structural changes in aged mice |
| topic | aging beetroot endothelial dysfunction medial thickening nitrate oxidative stress |
| url | https://doi.org/10.14814/phy2.15755 |
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