Proteomics and phosphoproteomics to study Tuina reverses capsule fibrosis in frozen shoulder: a research report based on rats
Abstract Frozen shoulder (FS) is a common disorder often treated with Tuina, but the mechanisms involved remain unclear. We employed proteomics and phosphoproteomics to investigate the mechanisms associated with the treatment of capsule fibrosis in FS rats. We used a method composed of three weeks o...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2024-01-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-50904-9 |
| _version_ | 1797363394580316160 |
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| author | Yingjie Qiao Jianmin Wang Lijun Zheng Yanhong Yang Huadong Li Muzhen Li Shidong Zhang Hongyi Wang Tiantian Zhang |
| author_facet | Yingjie Qiao Jianmin Wang Lijun Zheng Yanhong Yang Huadong Li Muzhen Li Shidong Zhang Hongyi Wang Tiantian Zhang |
| author_sort | Yingjie Qiao |
| collection | DOAJ |
| description | Abstract Frozen shoulder (FS) is a common disorder often treated with Tuina, but the mechanisms involved remain unclear. We employed proteomics and phosphoproteomics to investigate the mechanisms associated with the treatment of capsule fibrosis in FS rats. We used a method composed of three weeks of cast immobilization to establish a model of FS. We then administered Tuina once daily for 14 days, evaluated glenohumeral range of motion (ROM), assessed histological changes, and identified differentially expressed proteins (DEPs) using proteomics and phosphoproteomics. This study demonstrated that Tuina could improve glenohumeral ROM and reserve capsule fibrosis in FS rats. Proteomics revealed proteins regulated by Tuina belonging to the PI3K-AKT and ECM receptor interaction signaling pathways. Phosphoproteomics detected differentially phosphorylated proteins regulated by Tuina to be enriched in the MAPK signaling pathway. The combination of proteomics and phosphoproteomics for Protein–Protein Interaction (PPI) network analysis revealed that the phosphorylation of Myh3 and Srsf1 with a node degree larger than the average degree were considered the central regulatory protein modulated by Tuina to reverse capsule fibrosis. Thbs1, Vtn, and Tenascin-W were significantly enriched in PI3K-AKT and ECM receptor interaction signaling pathways and highly expressed in model rats. Tuina resulted in reduced expression of these proteins. Our findings demonstrated some of mechanisms behind the reversal of FS capsule fibrosis following Tuina, a scientific medical therapy for FS patients. |
| first_indexed | 2024-03-08T16:19:49Z |
| format | Article |
| id | doaj.art-c1e2dd5b17ea45a8ae2aad8a025f1a49 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-08T16:19:49Z |
| publishDate | 2024-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-c1e2dd5b17ea45a8ae2aad8a025f1a492024-01-07T12:25:48ZengNature PortfolioScientific Reports2045-23222024-01-0114111210.1038/s41598-023-50904-9Proteomics and phosphoproteomics to study Tuina reverses capsule fibrosis in frozen shoulder: a research report based on ratsYingjie Qiao0Jianmin Wang1Lijun Zheng2Yanhong Yang3Huadong Li4Muzhen Li5Shidong Zhang6Hongyi Wang7Tiantian Zhang8College of Acupuncture and Tuina, Shandong University of Traditional Chinese MedicineDepartment of Tuina, Shandong University of Traditional Chinese Medicine Affiliated HospitalCollege of Acupuncture and Tuina, Shandong University of Traditional Chinese MedicineSpecial Education and Rehabilitation College, Binzhou Medical UniversityDepartment of Tuina, Shandong University of Traditional Chinese Medicine Affiliated HospitalCollege of Acupuncture and Tuina, Shandong University of Traditional Chinese MedicineCollege of Acupuncture and Tuina, Shandong University of Traditional Chinese MedicineCollege of Acupuncture and Tuina, Shandong University of Traditional Chinese MedicineCollege of Acupuncture and Tuina, Shandong University of Traditional Chinese MedicineAbstract Frozen shoulder (FS) is a common disorder often treated with Tuina, but the mechanisms involved remain unclear. We employed proteomics and phosphoproteomics to investigate the mechanisms associated with the treatment of capsule fibrosis in FS rats. We used a method composed of three weeks of cast immobilization to establish a model of FS. We then administered Tuina once daily for 14 days, evaluated glenohumeral range of motion (ROM), assessed histological changes, and identified differentially expressed proteins (DEPs) using proteomics and phosphoproteomics. This study demonstrated that Tuina could improve glenohumeral ROM and reserve capsule fibrosis in FS rats. Proteomics revealed proteins regulated by Tuina belonging to the PI3K-AKT and ECM receptor interaction signaling pathways. Phosphoproteomics detected differentially phosphorylated proteins regulated by Tuina to be enriched in the MAPK signaling pathway. The combination of proteomics and phosphoproteomics for Protein–Protein Interaction (PPI) network analysis revealed that the phosphorylation of Myh3 and Srsf1 with a node degree larger than the average degree were considered the central regulatory protein modulated by Tuina to reverse capsule fibrosis. Thbs1, Vtn, and Tenascin-W were significantly enriched in PI3K-AKT and ECM receptor interaction signaling pathways and highly expressed in model rats. Tuina resulted in reduced expression of these proteins. Our findings demonstrated some of mechanisms behind the reversal of FS capsule fibrosis following Tuina, a scientific medical therapy for FS patients.https://doi.org/10.1038/s41598-023-50904-9 |
| spellingShingle | Yingjie Qiao Jianmin Wang Lijun Zheng Yanhong Yang Huadong Li Muzhen Li Shidong Zhang Hongyi Wang Tiantian Zhang Proteomics and phosphoproteomics to study Tuina reverses capsule fibrosis in frozen shoulder: a research report based on rats Scientific Reports |
| title | Proteomics and phosphoproteomics to study Tuina reverses capsule fibrosis in frozen shoulder: a research report based on rats |
| title_full | Proteomics and phosphoproteomics to study Tuina reverses capsule fibrosis in frozen shoulder: a research report based on rats |
| title_fullStr | Proteomics and phosphoproteomics to study Tuina reverses capsule fibrosis in frozen shoulder: a research report based on rats |
| title_full_unstemmed | Proteomics and phosphoproteomics to study Tuina reverses capsule fibrosis in frozen shoulder: a research report based on rats |
| title_short | Proteomics and phosphoproteomics to study Tuina reverses capsule fibrosis in frozen shoulder: a research report based on rats |
| title_sort | proteomics and phosphoproteomics to study tuina reverses capsule fibrosis in frozen shoulder a research report based on rats |
| url | https://doi.org/10.1038/s41598-023-50904-9 |
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