Accumulation-associated Protein Gene and TGF-beta 1 Affects the Formation of Lung Cancer-related Biological Material Staphylococcus Epidermidis Biofilm
Background and objective This study was conducted to evaluate the effects of the accumulation-associated protein (Aap) gene and transform growth factor-beta 1 (TGF-β1) on the biofilm formation of lung cancer-related Staphylococcus epidermidis (SE). Methods Species identification was performed to iso...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | zho |
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Chinese Anti-Cancer Association; Chinese Antituberculosis Association
2014-04-01
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| Series: | Chinese Journal of Lung Cancer |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.3779/j.issn.1009-3419.2014.04.04 |
| _version_ | 1818084714101080064 |
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| author | Ying CHEN Yujie LEI Yunchao HUANG Zhongming RAO Lianhua YE Guangqiang ZHAO Xiaoyan WANG |
| author_facet | Ying CHEN Yujie LEI Yunchao HUANG Zhongming RAO Lianhua YE Guangqiang ZHAO Xiaoyan WANG |
| author_sort | Ying CHEN |
| collection | DOAJ |
| description | Background and objective This study was conducted to evaluate the effects of the accumulation-associated protein (Aap) gene and transform growth factor-beta 1 (TGF-β1) on the biofilm formation of lung cancer-related Staphylococcus epidermidis (SE). Methods Species identification was performed to isolate SE strains from clinically implanted materials in lung cancer patients. Stable genetic aggregated proteins, which are associated with negative and positive isolates, were obtained. The biofilm-formation ability of the SE Aap gene was determined by PCR. Density gradient method was used to extract peripheral blood mononuclear cells from patients with non-small cell lung cancer. After 30 h, these cells were co-cultured with A549 at different TGF-β1 concentrations. The supernatant was then combined with SE Aap+ and SE Aap- strains and co-cultured with a medical silicone rubber. A semi-quantitative adhesion test was performed for each bacterial biofilm formation. Scanning electron microscopy was also conducted to observe the microcosmic condition of this material on the bacterial biofilm surface. Results The Aap gene was closely related to SE biofilm formation. At 10 ng/mL, 20 ng/mL, and 40 ng/mL, SE Aap+ biofilm on the medical silicone rubber surface was thicker in the TGF-β1 group than in the control group. No significant differences were found between TGF-β1 groups. For the SE Aap- strains, no evident biofilm was formed in TGF-β1 and control groups. Conclusion In plant material-related infection of lung cancer patients, SE Aap+ strain easily forms biofilm. Furthermore, TGF-β1 was conducive for the biofilm formation of SE Aap+ strains. |
| first_indexed | 2024-12-10T19:58:16Z |
| format | Article |
| id | doaj.art-c1e39eafeee64d129cce9f70a6e69dd8 |
| institution | Directory Open Access Journal |
| issn | 1009-3419 |
| language | zho |
| last_indexed | 2024-12-10T19:58:16Z |
| publishDate | 2014-04-01 |
| publisher | Chinese Anti-Cancer Association; Chinese Antituberculosis Association |
| record_format | Article |
| series | Chinese Journal of Lung Cancer |
| spelling | doaj.art-c1e39eafeee64d129cce9f70a6e69dd82022-12-22T01:35:36ZzhoChinese Anti-Cancer Association; Chinese Antituberculosis AssociationChinese Journal of Lung Cancer1009-34192014-04-0117430831410.3779/j.issn.1009-3419.2014.04.04Accumulation-associated Protein Gene and TGF-beta 1 Affects the Formation of Lung Cancer-related Biological Material Staphylococcus Epidermidis BiofilmYing CHEN0Yujie LEI1Yunchao HUANG2Zhongming RAO3Lianhua YE4Guangqiang ZHAO5Xiaoyan WANG6Deparment of Thoracic Surgery, the Third Affiliated Hospital of Kunming Medical University, Tumor Hospital of Yunnan Province, Kunming 650118, ChinaDeparment of Thoracic Surgery, the Third Affiliated Hospital of Kunming Medical University, Tumor Hospital of Yunnan Province, Kunming 650118, ChinaDeparment of Thoracic Surgery, the Third Affiliated Hospital of Kunming Medical University, Tumor Hospital of Yunnan Province, Kunming 650118, ChinaDeparment of Thoracic Surgery, the Third Affiliated Hospital of Kunming Medical University, Tumor Hospital of Yunnan Province, Kunming 650118, ChinaDeparment of Thoracic Surgery, the Third Affiliated Hospital of Kunming Medical University, Tumor Hospital of Yunnan Province, Kunming 650118, ChinaDeparment of Thoracic Surgery, the Third Affiliated Hospital of Kunming Medical University, Tumor Hospital of Yunnan Province, Kunming 650118, ChinaDeparment of Cardiovascular Surgery, the Affiliated Yan’an Hospital of Kunming Medical University,
Kunming 650051, ChinaBackground and objective This study was conducted to evaluate the effects of the accumulation-associated protein (Aap) gene and transform growth factor-beta 1 (TGF-β1) on the biofilm formation of lung cancer-related Staphylococcus epidermidis (SE). Methods Species identification was performed to isolate SE strains from clinically implanted materials in lung cancer patients. Stable genetic aggregated proteins, which are associated with negative and positive isolates, were obtained. The biofilm-formation ability of the SE Aap gene was determined by PCR. Density gradient method was used to extract peripheral blood mononuclear cells from patients with non-small cell lung cancer. After 30 h, these cells were co-cultured with A549 at different TGF-β1 concentrations. The supernatant was then combined with SE Aap+ and SE Aap- strains and co-cultured with a medical silicone rubber. A semi-quantitative adhesion test was performed for each bacterial biofilm formation. Scanning electron microscopy was also conducted to observe the microcosmic condition of this material on the bacterial biofilm surface. Results The Aap gene was closely related to SE biofilm formation. At 10 ng/mL, 20 ng/mL, and 40 ng/mL, SE Aap+ biofilm on the medical silicone rubber surface was thicker in the TGF-β1 group than in the control group. No significant differences were found between TGF-β1 groups. For the SE Aap- strains, no evident biofilm was formed in TGF-β1 and control groups. Conclusion In plant material-related infection of lung cancer patients, SE Aap+ strain easily forms biofilm. Furthermore, TGF-β1 was conducive for the biofilm formation of SE Aap+ strains.http://dx.doi.org/10.3779/j.issn.1009-3419.2014.04.04Lung neoplasmsMedical grade silicon rubberStaphylococcus epidermidisBiofilmAccumulation associated protein geneTGF-β1 |
| spellingShingle | Ying CHEN Yujie LEI Yunchao HUANG Zhongming RAO Lianhua YE Guangqiang ZHAO Xiaoyan WANG Accumulation-associated Protein Gene and TGF-beta 1 Affects the Formation of Lung Cancer-related Biological Material Staphylococcus Epidermidis Biofilm Chinese Journal of Lung Cancer Lung neoplasms Medical grade silicon rubber Staphylococcus epidermidis Biofilm Accumulation associated protein gene TGF-β1 |
| title | Accumulation-associated Protein Gene and TGF-beta 1 Affects the Formation of Lung Cancer-related Biological Material Staphylococcus Epidermidis Biofilm |
| title_full | Accumulation-associated Protein Gene and TGF-beta 1 Affects the Formation of Lung Cancer-related Biological Material Staphylococcus Epidermidis Biofilm |
| title_fullStr | Accumulation-associated Protein Gene and TGF-beta 1 Affects the Formation of Lung Cancer-related Biological Material Staphylococcus Epidermidis Biofilm |
| title_full_unstemmed | Accumulation-associated Protein Gene and TGF-beta 1 Affects the Formation of Lung Cancer-related Biological Material Staphylococcus Epidermidis Biofilm |
| title_short | Accumulation-associated Protein Gene and TGF-beta 1 Affects the Formation of Lung Cancer-related Biological Material Staphylococcus Epidermidis Biofilm |
| title_sort | accumulation associated protein gene and tgf beta 1 affects the formation of lung cancer related biological material staphylococcus epidermidis biofilm |
| topic | Lung neoplasms Medical grade silicon rubber Staphylococcus epidermidis Biofilm Accumulation associated protein gene TGF-β1 |
| url | http://dx.doi.org/10.3779/j.issn.1009-3419.2014.04.04 |
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