A prospective cohort study of MTHFR C677T gene polymorphism and its influence on the therapeutic effect of homocysteine in stroke patients with hyperhomocysteinemia
Abstract Background Hyperhomocysteinemia (HHCY) is a risk factor for cardiovascular and cerebrovascular diseases. The C677T 5, 10-methylenetetrahydrofolate reductase (MTHFR) gene polymorphism increases homocysteine (HCY) levels. This study analyzed the relationship between C677T MTHFR polymorphism a...
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BMC
2020-04-01
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| Series: | BMC Neurology |
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| Online Access: | http://link.springer.com/article/10.1186/s12883-020-01701-8 |
| _version_ | 1818930485378678784 |
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| author | Xiaoxia Du Lin Xiao Rong Sun Kunpeng Li Lin Liang Luping Song Zhizhong Liu |
| author_facet | Xiaoxia Du Lin Xiao Rong Sun Kunpeng Li Lin Liang Luping Song Zhizhong Liu |
| author_sort | Xiaoxia Du |
| collection | DOAJ |
| description | Abstract Background Hyperhomocysteinemia (HHCY) is a risk factor for cardiovascular and cerebrovascular diseases. The C677T 5, 10-methylenetetrahydrofolate reductase (MTHFR) gene polymorphism increases homocysteine (HCY) levels. This study analyzed the relationship between C677T MTHFR polymorphism and the therapeutic effect of lowering HCY in stroke patients with HHCY. Methods Baseline data were collected from stroke patients with HHCY for this prospective cohort study. The C677T MTHFR genotype was detected by polymerase chain reaction-restriction fragment length polymorphism and the therapeutic effect to reduce HCY was compared. Results Of 200 stroke patients 162 (81.0%) completed follow-up and were evaluated. Most of them responded well to treatment (103 cases, 63.5%), but 59 (36.4%) patients were in the poor efficacy group. There was a significant difference in terms of age (P < 0.001), hypertension (P = 0.041), hyperuricemia (P = 0.042), HCY after treatment (P < 0.001), and MTHFR genotype (P < 0.001) between the poor efficacy and effective groups, with increased frequency of the TT genotype in the poor efficacy group. Logistic regression showed that the T allele was associated with poor efficacy (OR = 0.733, 95%CI: 0.693, 0.862, P < 0.001). In the codominant model the TT genotype was associated with poor outcome (OR = 0.862, 95%CI: 0.767, 0.970, P = 0.017) and this was also the case in the recessive model (OR = 0.585, 95%CI: 0.462, 0.741, P < 0.001) but there was no association between CT and TT in the dominant model. Conclusions The T allele and TT genotype of the MTHFR C677T polymorphism was associated with poor HCY reduction treatment efficacy in stroke patients with HHCY. Trial registration The registration number of the clinical trial is ChiCTR1800020048 . Registration date: December 12, 2018. |
| first_indexed | 2024-12-20T04:01:27Z |
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| institution | Directory Open Access Journal |
| issn | 1471-2377 |
| language | English |
| last_indexed | 2024-12-20T04:01:27Z |
| publishDate | 2020-04-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Neurology |
| spelling | doaj.art-c1e8449bd789411682df12a8f3fcf82b2022-12-21T19:54:10ZengBMCBMC Neurology1471-23772020-04-012011710.1186/s12883-020-01701-8A prospective cohort study of MTHFR C677T gene polymorphism and its influence on the therapeutic effect of homocysteine in stroke patients with hyperhomocysteinemiaXiaoxia Du0Lin Xiao1Rong Sun2Kunpeng Li3Lin Liang4Luping Song5Zhizhong Liu6Department of Neurorehabilitation, School of Rehabilitation Medicine, Capital Medical University, Beijing Bo’Ai Hospital, China Rehabilitation Research CenterDepartment of Neurorehabilitation, School of Rehabilitation Medicine, Capital Medical University, Beijing Bo’Ai Hospital, China Rehabilitation Research CenterDepartment of Neurorehabilitation, School of Rehabilitation Medicine, Capital Medical University, Beijing Bo’Ai Hospital, China Rehabilitation Research CenterCase Statistics Office, School of Rehabilitation Medicine, Capital Medical University, Beijing Bo’Ai Hospital, China Rehabilitation Research CenterDepartment of Clinical Laboratory, School of Rehabilitation Medicine, Capital Medical University, Beijing Bo’Ai Hospital, China Rehabilitation Research CenterDepartment of Neurorehabilitation, School of Rehabilitation Medicine, Capital Medical University, Beijing Bo’Ai Hospital, China Rehabilitation Research CenterDepartment of Clinical Laboratory, School of Rehabilitation Medicine, Capital Medical University, Beijing Bo’Ai Hospital, China Rehabilitation Research CenterAbstract Background Hyperhomocysteinemia (HHCY) is a risk factor for cardiovascular and cerebrovascular diseases. The C677T 5, 10-methylenetetrahydrofolate reductase (MTHFR) gene polymorphism increases homocysteine (HCY) levels. This study analyzed the relationship between C677T MTHFR polymorphism and the therapeutic effect of lowering HCY in stroke patients with HHCY. Methods Baseline data were collected from stroke patients with HHCY for this prospective cohort study. The C677T MTHFR genotype was detected by polymerase chain reaction-restriction fragment length polymorphism and the therapeutic effect to reduce HCY was compared. Results Of 200 stroke patients 162 (81.0%) completed follow-up and were evaluated. Most of them responded well to treatment (103 cases, 63.5%), but 59 (36.4%) patients were in the poor efficacy group. There was a significant difference in terms of age (P < 0.001), hypertension (P = 0.041), hyperuricemia (P = 0.042), HCY after treatment (P < 0.001), and MTHFR genotype (P < 0.001) between the poor efficacy and effective groups, with increased frequency of the TT genotype in the poor efficacy group. Logistic regression showed that the T allele was associated with poor efficacy (OR = 0.733, 95%CI: 0.693, 0.862, P < 0.001). In the codominant model the TT genotype was associated with poor outcome (OR = 0.862, 95%CI: 0.767, 0.970, P = 0.017) and this was also the case in the recessive model (OR = 0.585, 95%CI: 0.462, 0.741, P < 0.001) but there was no association between CT and TT in the dominant model. Conclusions The T allele and TT genotype of the MTHFR C677T polymorphism was associated with poor HCY reduction treatment efficacy in stroke patients with HHCY. Trial registration The registration number of the clinical trial is ChiCTR1800020048 . Registration date: December 12, 2018.http://link.springer.com/article/10.1186/s12883-020-01701-8HomocysteineStrokeIntracerebral hemorrhageMethylenetetrahydrofolate reductaseSingle nucleotide polymorphism |
| spellingShingle | Xiaoxia Du Lin Xiao Rong Sun Kunpeng Li Lin Liang Luping Song Zhizhong Liu A prospective cohort study of MTHFR C677T gene polymorphism and its influence on the therapeutic effect of homocysteine in stroke patients with hyperhomocysteinemia BMC Neurology Homocysteine Stroke Intracerebral hemorrhage Methylenetetrahydrofolate reductase Single nucleotide polymorphism |
| title | A prospective cohort study of MTHFR C677T gene polymorphism and its influence on the therapeutic effect of homocysteine in stroke patients with hyperhomocysteinemia |
| title_full | A prospective cohort study of MTHFR C677T gene polymorphism and its influence on the therapeutic effect of homocysteine in stroke patients with hyperhomocysteinemia |
| title_fullStr | A prospective cohort study of MTHFR C677T gene polymorphism and its influence on the therapeutic effect of homocysteine in stroke patients with hyperhomocysteinemia |
| title_full_unstemmed | A prospective cohort study of MTHFR C677T gene polymorphism and its influence on the therapeutic effect of homocysteine in stroke patients with hyperhomocysteinemia |
| title_short | A prospective cohort study of MTHFR C677T gene polymorphism and its influence on the therapeutic effect of homocysteine in stroke patients with hyperhomocysteinemia |
| title_sort | prospective cohort study of mthfr c677t gene polymorphism and its influence on the therapeutic effect of homocysteine in stroke patients with hyperhomocysteinemia |
| topic | Homocysteine Stroke Intracerebral hemorrhage Methylenetetrahydrofolate reductase Single nucleotide polymorphism |
| url | http://link.springer.com/article/10.1186/s12883-020-01701-8 |
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