Genetic engineering of non-native hosts for 1-butanol production and its challenges: a review
Abstract Background Owing to the increase in energy consumption, fossil fuel resources are gradually depleting which has led to the growing environmental concerns; therefore, scientists are being urged to produce sustainable and ecofriendly fuels. Thus, there is a growing interest in the generation...
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| Format: | Article |
| Language: | English |
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BMC
2020-03-01
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| Series: | Microbial Cell Factories |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12934-020-01337-w |
| _version_ | 1828266672887169024 |
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| author | Said Nawab Ning Wang Xiaoyan Ma Yi-Xin Huo |
| author_facet | Said Nawab Ning Wang Xiaoyan Ma Yi-Xin Huo |
| author_sort | Said Nawab |
| collection | DOAJ |
| description | Abstract Background Owing to the increase in energy consumption, fossil fuel resources are gradually depleting which has led to the growing environmental concerns; therefore, scientists are being urged to produce sustainable and ecofriendly fuels. Thus, there is a growing interest in the generation of biofuels from renewable energy resources using microbial fermentation. Main text Butanol is a promising biofuel that can substitute for gasoline; unfortunately, natural microorganisms pose challenges for the economical production of 1-butanol at an industrial scale. The availability of genetic and molecular tools to engineer existing native pathways or create synthetic pathways have made non-native hosts a good choice for the production of 1-butanol from renewable resources. Non-native hosts have several distinct advantages, including using of cost-efficient feedstock, solvent tolerant and reduction of contamination risk. Therefore, engineering non-native hosts to produce biofuels is a promising approach towards achieving sustainability. This paper reviews the currently employed strategies and synthetic biology approaches used to produce 1-butanol in non-native hosts over the past few years. In addition, current challenges faced in using non-native hosts and the possible solutions that can help improve 1-butanol production are also discussed. Conclusion Non-native organisms have the potential to realize commercial production of 1- butanol from renewable resources. Future research should focus on substrate utilization, cofactor imbalance, and promoter selection to boost 1-butanol production in non-native hosts. Moreover, the application of robust genetic engineering approaches is required for metabolic engineering of microorganisms to make them industrially feasible for 1-butanol production. |
| first_indexed | 2024-04-13T04:56:29Z |
| format | Article |
| id | doaj.art-c1ea50aa75b74bca99792065786f2efc |
| institution | Directory Open Access Journal |
| issn | 1475-2859 |
| language | English |
| last_indexed | 2024-04-13T04:56:29Z |
| publishDate | 2020-03-01 |
| publisher | BMC |
| record_format | Article |
| series | Microbial Cell Factories |
| spelling | doaj.art-c1ea50aa75b74bca99792065786f2efc2022-12-22T03:01:29ZengBMCMicrobial Cell Factories1475-28592020-03-0119111610.1186/s12934-020-01337-wGenetic engineering of non-native hosts for 1-butanol production and its challenges: a reviewSaid Nawab0Ning Wang1Xiaoyan Ma2Yi-Xin Huo3Key Laboratory of Molecular Medicine and Biotherapy, School of Life Science, Beijing Institute of TechnologyKey Laboratory of Molecular Medicine and Biotherapy, School of Life Science, Beijing Institute of TechnologyKey Laboratory of Molecular Medicine and Biotherapy, School of Life Science, Beijing Institute of TechnologyKey Laboratory of Molecular Medicine and Biotherapy, School of Life Science, Beijing Institute of TechnologyAbstract Background Owing to the increase in energy consumption, fossil fuel resources are gradually depleting which has led to the growing environmental concerns; therefore, scientists are being urged to produce sustainable and ecofriendly fuels. Thus, there is a growing interest in the generation of biofuels from renewable energy resources using microbial fermentation. Main text Butanol is a promising biofuel that can substitute for gasoline; unfortunately, natural microorganisms pose challenges for the economical production of 1-butanol at an industrial scale. The availability of genetic and molecular tools to engineer existing native pathways or create synthetic pathways have made non-native hosts a good choice for the production of 1-butanol from renewable resources. Non-native hosts have several distinct advantages, including using of cost-efficient feedstock, solvent tolerant and reduction of contamination risk. Therefore, engineering non-native hosts to produce biofuels is a promising approach towards achieving sustainability. This paper reviews the currently employed strategies and synthetic biology approaches used to produce 1-butanol in non-native hosts over the past few years. In addition, current challenges faced in using non-native hosts and the possible solutions that can help improve 1-butanol production are also discussed. Conclusion Non-native organisms have the potential to realize commercial production of 1- butanol from renewable resources. Future research should focus on substrate utilization, cofactor imbalance, and promoter selection to boost 1-butanol production in non-native hosts. Moreover, the application of robust genetic engineering approaches is required for metabolic engineering of microorganisms to make them industrially feasible for 1-butanol production.http://link.springer.com/article/10.1186/s12934-020-01337-w1-ButanolNon-native hostsBiofuel productionSynthetic pathways |
| spellingShingle | Said Nawab Ning Wang Xiaoyan Ma Yi-Xin Huo Genetic engineering of non-native hosts for 1-butanol production and its challenges: a review Microbial Cell Factories 1-Butanol Non-native hosts Biofuel production Synthetic pathways |
| title | Genetic engineering of non-native hosts for 1-butanol production and its challenges: a review |
| title_full | Genetic engineering of non-native hosts for 1-butanol production and its challenges: a review |
| title_fullStr | Genetic engineering of non-native hosts for 1-butanol production and its challenges: a review |
| title_full_unstemmed | Genetic engineering of non-native hosts for 1-butanol production and its challenges: a review |
| title_short | Genetic engineering of non-native hosts for 1-butanol production and its challenges: a review |
| title_sort | genetic engineering of non native hosts for 1 butanol production and its challenges a review |
| topic | 1-Butanol Non-native hosts Biofuel production Synthetic pathways |
| url | http://link.springer.com/article/10.1186/s12934-020-01337-w |
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