Determination of 6 Kinds of Veterinary Drug Residues in Milk by Ultra Performance Liquid Chromatography-tandem Mass Spectrometry
Objective: To establish a high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method for the determination of six veterinary drug residues in milk samples. Methods: The sample was extracted with acetonitrile solution, purified with MAX solid phase extraction column, and sepa...
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| Format: | Article |
| Language: | zho |
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The editorial department of Science and Technology of Food Industry
2022-03-01
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| Series: | Shipin gongye ke-ji |
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| Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2020110232 |
| _version_ | 1798017471013191680 |
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| author | Min ZHANG Ge WANG Xiaochong MA Yaoguang ZHANG Yanbing CHAI Xingjia LI Fei LI |
| author_facet | Min ZHANG Ge WANG Xiaochong MA Yaoguang ZHANG Yanbing CHAI Xingjia LI Fei LI |
| author_sort | Min ZHANG |
| collection | DOAJ |
| description | Objective: To establish a high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method for the determination of six veterinary drug residues in milk samples. Methods: The sample was extracted with acetonitrile solution, purified with MAX solid phase extraction column, and separated by Waters X Bridge BEH-C18 chromatographic column. The mobile phase was eluted with a gradient of acetonitrile and 0.1% formic acid aqueous solution, and electrospray ion source was used, positive and negative ion switching scanning mode for detection, external standard method for quantification. Results: The 6 veterinary drugs showed a good linear relationship in the range of 0~10 ng/mL, and the coefficient of determination (R2) was greater than 0.995. The detection limit of the 6 veterinary drugs was 0.06~0.18 μg/kg. The limit of quantification of the method was 0.5~2.0 μg/kg. With the addition of 0.5~8.0 μg/kg in the standard recovery experiment, the recovery rates of the 6 veterinary drugs were 67.1%~105.5%, and the relative standard deviations were all less than 10%. Conclusion: This method is easy to operate, fast in analysis, and high in sensitivity. It can be used for the determination of veterinary drug residues in milk samples. |
| first_indexed | 2024-04-11T16:07:46Z |
| format | Article |
| id | doaj.art-c1ea8b2e2fb74e53a216e0bfa6111725 |
| institution | Directory Open Access Journal |
| issn | 1002-0306 |
| language | zho |
| last_indexed | 2024-04-11T16:07:46Z |
| publishDate | 2022-03-01 |
| publisher | The editorial department of Science and Technology of Food Industry |
| record_format | Article |
| series | Shipin gongye ke-ji |
| spelling | doaj.art-c1ea8b2e2fb74e53a216e0bfa61117252022-12-22T04:14:47ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062022-03-0143525526110.13386/j.issn1002-0306.20201102322020110232-5Determination of 6 Kinds of Veterinary Drug Residues in Milk by Ultra Performance Liquid Chromatography-tandem Mass SpectrometryMin ZHANG0Ge WANG1Xiaochong MA2Yaoguang ZHANG3Yanbing CHAI4Xingjia LI5Fei LI6Key Laboratory of Dairy Quality and Safety Control, Ministry of Agriculture and Rural Affairs, Shijiazhuang Junlebao Dairy Co., Ltd., Shijiazhuang 050021, ChinaKey Laboratory of Dairy Quality and Safety Control, Ministry of Agriculture and Rural Affairs, Shijiazhuang Junlebao Dairy Co., Ltd., Shijiazhuang 050021, ChinaKey Laboratory of Dairy Quality and Safety Control, Ministry of Agriculture and Rural Affairs, Shijiazhuang Junlebao Dairy Co., Ltd., Shijiazhuang 050021, ChinaKey Laboratory of Dairy Quality and Safety Control, Ministry of Agriculture and Rural Affairs, Shijiazhuang Junlebao Dairy Co., Ltd., Shijiazhuang 050021, ChinaKey Laboratory of Dairy Quality and Safety Control, Ministry of Agriculture and Rural Affairs, Shijiazhuang Junlebao Dairy Co., Ltd., Shijiazhuang 050021, ChinaKey Laboratory of Dairy Quality and Safety Control, Ministry of Agriculture and Rural Affairs, Shijiazhuang Junlebao Dairy Co., Ltd., Shijiazhuang 050021, ChinaKey Laboratory of Dairy Quality and Safety Control, Ministry of Agriculture and Rural Affairs, Shijiazhuang Junlebao Dairy Co., Ltd., Shijiazhuang 050021, ChinaObjective: To establish a high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method for the determination of six veterinary drug residues in milk samples. Methods: The sample was extracted with acetonitrile solution, purified with MAX solid phase extraction column, and separated by Waters X Bridge BEH-C18 chromatographic column. The mobile phase was eluted with a gradient of acetonitrile and 0.1% formic acid aqueous solution, and electrospray ion source was used, positive and negative ion switching scanning mode for detection, external standard method for quantification. Results: The 6 veterinary drugs showed a good linear relationship in the range of 0~10 ng/mL, and the coefficient of determination (R2) was greater than 0.995. The detection limit of the 6 veterinary drugs was 0.06~0.18 μg/kg. The limit of quantification of the method was 0.5~2.0 μg/kg. With the addition of 0.5~8.0 μg/kg in the standard recovery experiment, the recovery rates of the 6 veterinary drugs were 67.1%~105.5%, and the relative standard deviations were all less than 10%. Conclusion: This method is easy to operate, fast in analysis, and high in sensitivity. It can be used for the determination of veterinary drug residues in milk samples.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2020110232high performance liquid chromatography-tandem mass spectrometryveterinary drug residuesolid phase extraction technologymilk |
| spellingShingle | Min ZHANG Ge WANG Xiaochong MA Yaoguang ZHANG Yanbing CHAI Xingjia LI Fei LI Determination of 6 Kinds of Veterinary Drug Residues in Milk by Ultra Performance Liquid Chromatography-tandem Mass Spectrometry Shipin gongye ke-ji high performance liquid chromatography-tandem mass spectrometry veterinary drug residue solid phase extraction technology milk |
| title | Determination of 6 Kinds of Veterinary Drug Residues in Milk by Ultra Performance Liquid Chromatography-tandem Mass Spectrometry |
| title_full | Determination of 6 Kinds of Veterinary Drug Residues in Milk by Ultra Performance Liquid Chromatography-tandem Mass Spectrometry |
| title_fullStr | Determination of 6 Kinds of Veterinary Drug Residues in Milk by Ultra Performance Liquid Chromatography-tandem Mass Spectrometry |
| title_full_unstemmed | Determination of 6 Kinds of Veterinary Drug Residues in Milk by Ultra Performance Liquid Chromatography-tandem Mass Spectrometry |
| title_short | Determination of 6 Kinds of Veterinary Drug Residues in Milk by Ultra Performance Liquid Chromatography-tandem Mass Spectrometry |
| title_sort | determination of 6 kinds of veterinary drug residues in milk by ultra performance liquid chromatography tandem mass spectrometry |
| topic | high performance liquid chromatography-tandem mass spectrometry veterinary drug residue solid phase extraction technology milk |
| url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2020110232 |
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