Detection of carbapenemase production in gram negative bacilli in medical and surgical intensive care unit patients in a tertiary care hospital
Background: Carbapenem-resistant Enterobacterales (CRE) are an urgent global public health problem. CRE infections are associated with high mortality and have limited available effective treatment. Carbapenemase enzymes are encoded by genes on mobile genetic elements, such as plasmids, which are hig...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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KIMS Foundation and Research Center
2023-12-01
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| Series: | Journal of Medical and Scientific Research |
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| Online Access: | http://www.jmsronline.com/archive-article/carbapenemase-gram-negative-bacilli-intensive-care-unit-patients |
| _version_ | 1827573099389779968 |
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| author | Kadel S Bilolikar AK Eswaran SP Krishnaveni M Fatima R Sahu S |
| author_facet | Kadel S Bilolikar AK Eswaran SP Krishnaveni M Fatima R Sahu S |
| author_sort | Kadel S |
| collection | DOAJ |
| description | Background: Carbapenem-resistant Enterobacterales (CRE) are an urgent global public health problem. CRE infections are associated with high mortality and have limited available effective treatment. Carbapenemase enzymes are encoded by genes on mobile genetic elements, such as plasmids, which are highly transmissible between organisms and increase the potential spread of resistance. The study aim was to detect carbapenemase production in Gram negative bacilli by phenotypic (CarbaNP and mCIM only or in conjunction with eCIM) and genotypic (Xpert Carba-R) methods.
Material and methods: The various clinical samples were processed as per standard recommended procedures. Identification and antibiotic susceptibility test were done by using GN cards and AST N280 & AST N281 cards of Vitek 2 Compact (bioMérieux) respectively. Phenotypic (CarbaNP and mCIM only or in conjunction with eCIM) and genotypic (Xpert Carba-R) methods were used for detection of carbapenemases.
Result: 144 carbapenem-resistant Gram-negative bacilli isolated from various ICUs includes Klebsiella pneumoniae 52.7% (76/144) followed by Escherichia coli 18.05% (26/144) and 15.9% (23/144) Acinetobacter baumannii. Phenotypic test (CarbaNP and mCIM and or in conjunction with eCIM) showed sensitivity of 90%, 100% and 93.75% respectively. Genotypic test of 40 isolates showed predominant expression of NDM in 82.5% (33/40) isolates followed by OXA-48 in 40% (16/40).
Conclusion: The study showed mCIM as the most useful diagnostic test with less economic burden to the patients. There is an urgent need for more sensitive, rapid, highly precise and accurate genotypic test which is less expensive and less labor-intensive. |
| first_indexed | 2024-03-08T17:52:28Z |
| format | Article |
| id | doaj.art-c1f1e12d6f644b2aac4fb74cf04b79b8 |
| institution | Directory Open Access Journal |
| issn | 2321-1326 2394-112X |
| language | English |
| last_indexed | 2024-03-08T17:52:28Z |
| publishDate | 2023-12-01 |
| publisher | KIMS Foundation and Research Center |
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| series | Journal of Medical and Scientific Research |
| spelling | doaj.art-c1f1e12d6f644b2aac4fb74cf04b79b82024-01-02T07:17:40ZengKIMS Foundation and Research CenterJournal of Medical and Scientific Research2321-13262394-112X2023-12-0111430130910.17727/JMSR.2023/11-56Detection of carbapenemase production in gram negative bacilli in medical and surgical intensive care unit patients in a tertiary care hospitalKadel S0Bilolikar AK1Eswaran SP2Krishnaveni M3Fatima R4Sahu S5Department of Microbiology, Krishna Institute of Medical Sciences, Minister Road, Secunderabad-500003, Telangana, IndiaDepartment of Microbiology, Krishna Institute of Medical Sciences, Minister Road, Secunderabad-500003, Telangana, IndiaDepartment of Microbiology, Krishna Institute of Medical Sciences, Minister Road, Secunderabad-500003, Telangana, IndiaDepartment of Microbiology, Krishna Institute of Medical Sciences, Minister Road, Secunderabad-500003, Telangana, IndiaDepartment of Microbiology, Krishna Institute of Medical Sciences, Minister Road, Secunderabad-500003, Telangana, IndiaDepartment of Critical Care, Krishna Institute of Medical Sciences, Minister Road, Secunderabad-500003, Telangana, IndiaBackground: Carbapenem-resistant Enterobacterales (CRE) are an urgent global public health problem. CRE infections are associated with high mortality and have limited available effective treatment. Carbapenemase enzymes are encoded by genes on mobile genetic elements, such as plasmids, which are highly transmissible between organisms and increase the potential spread of resistance. The study aim was to detect carbapenemase production in Gram negative bacilli by phenotypic (CarbaNP and mCIM only or in conjunction with eCIM) and genotypic (Xpert Carba-R) methods. Material and methods: The various clinical samples were processed as per standard recommended procedures. Identification and antibiotic susceptibility test were done by using GN cards and AST N280 & AST N281 cards of Vitek 2 Compact (bioMérieux) respectively. Phenotypic (CarbaNP and mCIM only or in conjunction with eCIM) and genotypic (Xpert Carba-R) methods were used for detection of carbapenemases. Result: 144 carbapenem-resistant Gram-negative bacilli isolated from various ICUs includes Klebsiella pneumoniae 52.7% (76/144) followed by Escherichia coli 18.05% (26/144) and 15.9% (23/144) Acinetobacter baumannii. Phenotypic test (CarbaNP and mCIM and or in conjunction with eCIM) showed sensitivity of 90%, 100% and 93.75% respectively. Genotypic test of 40 isolates showed predominant expression of NDM in 82.5% (33/40) isolates followed by OXA-48 in 40% (16/40). Conclusion: The study showed mCIM as the most useful diagnostic test with less economic burden to the patients. There is an urgent need for more sensitive, rapid, highly precise and accurate genotypic test which is less expensive and less labor-intensive.http://www.jmsronline.com/archive-article/carbapenemase-gram-negative-bacilli-intensive-care-unit-patientscarbapenem resistantenterobacteralesmcimecimxpert carba-r |
| spellingShingle | Kadel S Bilolikar AK Eswaran SP Krishnaveni M Fatima R Sahu S Detection of carbapenemase production in gram negative bacilli in medical and surgical intensive care unit patients in a tertiary care hospital Journal of Medical and Scientific Research carbapenem resistant enterobacterales mcim ecim xpert carba-r |
| title | Detection of carbapenemase production in gram negative bacilli in medical and surgical intensive care unit patients in a tertiary care hospital |
| title_full | Detection of carbapenemase production in gram negative bacilli in medical and surgical intensive care unit patients in a tertiary care hospital |
| title_fullStr | Detection of carbapenemase production in gram negative bacilli in medical and surgical intensive care unit patients in a tertiary care hospital |
| title_full_unstemmed | Detection of carbapenemase production in gram negative bacilli in medical and surgical intensive care unit patients in a tertiary care hospital |
| title_short | Detection of carbapenemase production in gram negative bacilli in medical and surgical intensive care unit patients in a tertiary care hospital |
| title_sort | detection of carbapenemase production in gram negative bacilli in medical and surgical intensive care unit patients in a tertiary care hospital |
| topic | carbapenem resistant enterobacterales mcim ecim xpert carba-r |
| url | http://www.jmsronline.com/archive-article/carbapenemase-gram-negative-bacilli-intensive-care-unit-patients |
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