A novel compound from the mushroom Cryptoporus volvatus inhibits porcine reproductive and respiratory syndrome virus (PRRSV) in vitro.
Porcine reproductive and respiratory syndrome (PRRS), caused by PRRS virus (PRRSV), is a serious contagious disease in the swine industry. At present, there are no effective control strategies against PRRSV. Thus, there is an urgent need for new treatment regimens that have efficacious antiviral act...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2013-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3832501?pdf=render |
| _version_ | 1819060144480190464 |
|---|---|
| author | Zengqiang Ma Weiwei Zhang Li Wang Mengjuan Zhu Hexiang Wang Wen-Hai Feng Tzi Bun Ng |
| author_facet | Zengqiang Ma Weiwei Zhang Li Wang Mengjuan Zhu Hexiang Wang Wen-Hai Feng Tzi Bun Ng |
| author_sort | Zengqiang Ma |
| collection | DOAJ |
| description | Porcine reproductive and respiratory syndrome (PRRS), caused by PRRS virus (PRRSV), is a serious contagious disease in the swine industry. At present, there are no effective control strategies against PRRSV. Thus, there is an urgent need for new treatment regimens that have efficacious antiviral activity to compensate for vaccines. The anti-infective effect of Cryptoporus volvatus has previously been demonstrated in Tradational Chinese Medicine. In this report, we expected to identify a new anti-PRRSV agent in the aqueous extract of C. volvatus, by employing a combination of modern chromatographic purification techniques and indirect immunofluorescence assay (IFA). Our results showed that C. volvatus extracts from every separation step differed in their inhibitory potency on PRRSV. One anti-PRRSV component designated as CM-H-L-5 was isolated from water-soluble fraction of C. volvatus. The inhibition induced by CM-H-L-5 occurred in a dose-dependent manner. CM-H-L-5 appeared to be a low-molecular-weight polyol fragment with amide groups and carboxylic acid groups. Collectively, our findings imply that CM-H-L-5 from the aqueous extract of C. volvatus has the potential to be used for anti-PRRSV therapy. |
| first_indexed | 2024-12-21T14:22:19Z |
| format | Article |
| id | doaj.art-c1f9070dcb6349b2bb6bc90a9b6e9db6 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-21T14:22:19Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-c1f9070dcb6349b2bb6bc90a9b6e9db62022-12-21T19:00:44ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-01811e7933310.1371/journal.pone.0079333A novel compound from the mushroom Cryptoporus volvatus inhibits porcine reproductive and respiratory syndrome virus (PRRSV) in vitro.Zengqiang MaWeiwei ZhangLi WangMengjuan ZhuHexiang WangWen-Hai FengTzi Bun NgPorcine reproductive and respiratory syndrome (PRRS), caused by PRRS virus (PRRSV), is a serious contagious disease in the swine industry. At present, there are no effective control strategies against PRRSV. Thus, there is an urgent need for new treatment regimens that have efficacious antiviral activity to compensate for vaccines. The anti-infective effect of Cryptoporus volvatus has previously been demonstrated in Tradational Chinese Medicine. In this report, we expected to identify a new anti-PRRSV agent in the aqueous extract of C. volvatus, by employing a combination of modern chromatographic purification techniques and indirect immunofluorescence assay (IFA). Our results showed that C. volvatus extracts from every separation step differed in their inhibitory potency on PRRSV. One anti-PRRSV component designated as CM-H-L-5 was isolated from water-soluble fraction of C. volvatus. The inhibition induced by CM-H-L-5 occurred in a dose-dependent manner. CM-H-L-5 appeared to be a low-molecular-weight polyol fragment with amide groups and carboxylic acid groups. Collectively, our findings imply that CM-H-L-5 from the aqueous extract of C. volvatus has the potential to be used for anti-PRRSV therapy.http://europepmc.org/articles/PMC3832501?pdf=render |
| spellingShingle | Zengqiang Ma Weiwei Zhang Li Wang Mengjuan Zhu Hexiang Wang Wen-Hai Feng Tzi Bun Ng A novel compound from the mushroom Cryptoporus volvatus inhibits porcine reproductive and respiratory syndrome virus (PRRSV) in vitro. PLoS ONE |
| title | A novel compound from the mushroom Cryptoporus volvatus inhibits porcine reproductive and respiratory syndrome virus (PRRSV) in vitro. |
| title_full | A novel compound from the mushroom Cryptoporus volvatus inhibits porcine reproductive and respiratory syndrome virus (PRRSV) in vitro. |
| title_fullStr | A novel compound from the mushroom Cryptoporus volvatus inhibits porcine reproductive and respiratory syndrome virus (PRRSV) in vitro. |
| title_full_unstemmed | A novel compound from the mushroom Cryptoporus volvatus inhibits porcine reproductive and respiratory syndrome virus (PRRSV) in vitro. |
| title_short | A novel compound from the mushroom Cryptoporus volvatus inhibits porcine reproductive and respiratory syndrome virus (PRRSV) in vitro. |
| title_sort | novel compound from the mushroom cryptoporus volvatus inhibits porcine reproductive and respiratory syndrome virus prrsv in vitro |
| url | http://europepmc.org/articles/PMC3832501?pdf=render |
| work_keys_str_mv | AT zengqiangma anovelcompoundfromthemushroomcryptoporusvolvatusinhibitsporcinereproductiveandrespiratorysyndromevirusprrsvinvitro AT weiweizhang anovelcompoundfromthemushroomcryptoporusvolvatusinhibitsporcinereproductiveandrespiratorysyndromevirusprrsvinvitro AT liwang anovelcompoundfromthemushroomcryptoporusvolvatusinhibitsporcinereproductiveandrespiratorysyndromevirusprrsvinvitro AT mengjuanzhu anovelcompoundfromthemushroomcryptoporusvolvatusinhibitsporcinereproductiveandrespiratorysyndromevirusprrsvinvitro AT hexiangwang anovelcompoundfromthemushroomcryptoporusvolvatusinhibitsporcinereproductiveandrespiratorysyndromevirusprrsvinvitro AT wenhaifeng anovelcompoundfromthemushroomcryptoporusvolvatusinhibitsporcinereproductiveandrespiratorysyndromevirusprrsvinvitro AT tzibunng anovelcompoundfromthemushroomcryptoporusvolvatusinhibitsporcinereproductiveandrespiratorysyndromevirusprrsvinvitro AT zengqiangma novelcompoundfromthemushroomcryptoporusvolvatusinhibitsporcinereproductiveandrespiratorysyndromevirusprrsvinvitro AT weiweizhang novelcompoundfromthemushroomcryptoporusvolvatusinhibitsporcinereproductiveandrespiratorysyndromevirusprrsvinvitro AT liwang novelcompoundfromthemushroomcryptoporusvolvatusinhibitsporcinereproductiveandrespiratorysyndromevirusprrsvinvitro AT mengjuanzhu novelcompoundfromthemushroomcryptoporusvolvatusinhibitsporcinereproductiveandrespiratorysyndromevirusprrsvinvitro AT hexiangwang novelcompoundfromthemushroomcryptoporusvolvatusinhibitsporcinereproductiveandrespiratorysyndromevirusprrsvinvitro AT wenhaifeng novelcompoundfromthemushroomcryptoporusvolvatusinhibitsporcinereproductiveandrespiratorysyndromevirusprrsvinvitro AT tzibunng novelcompoundfromthemushroomcryptoporusvolvatusinhibitsporcinereproductiveandrespiratorysyndromevirusprrsvinvitro |