MARKER ASSISTED SELECTION (MAS) FOR DEVELOPMENT OF BARLEY AND WHEAT LINES WITH REQUESTED TRAITS

Molecular markers closely linked to interesting genes enable early, proper and fast detection of plant individuals with desired allele during backcross breeding, what can make plant breeding faster and cheaper. We are focused on molecular breeding of barley and wheat lines for disease resistance and...

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Main Authors: M. Hudcovicová, S. Šliková, E. Gregová, D. Mihálik, S. Gavurníková, Z. Šramková
Format: Article
Language:English
Published: Faculty of Agrobitechnical Sciences Osijek 2008-09-01
Series:Poljoprivreda
Online Access:http://poljoprivreda.pfos.hr/upload/publications/poljoprivreda-14-1-sup17.pdf
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author M. Hudcovicová
S. Šliková
E. Gregová
D. Mihálik
S. Gavurníková
Z. Šramková
author_facet M. Hudcovicová
S. Šliková
E. Gregová
D. Mihálik
S. Gavurníková
Z. Šramková
author_sort M. Hudcovicová
collection DOAJ
description Molecular markers closely linked to interesting genes enable early, proper and fast detection of plant individuals with desired allele during backcross breeding, what can make plant breeding faster and cheaper. We are focused on molecular breeding of barley and wheat lines for disease resistance and some important quality traits. As acceptors of interesting genes we use especially elite Slovak and Czech cultivars and lines. After five backcross generations with the help of MAS new created lines carrying markers linked to desired genes undergo resistance, agronomic and technological tests. In breeding of winter barley for resistance to BaYMV/BaMMV viruses we use codominant STS and SSR markers linked to rym4 and rym11 resistance genes. Cultivar Romanze has been used as a donor of rym4 gene and landrace Russia57 as gene rym11 donor. In spring barley we are focused on transfer of Yd2 gene from landraces Shannon and Sutter resistant to BYDV by use of dominant ASPCR marker. We are also working on transfer of effective leaf rust resistance genes Lr19, Lr24 derived from Thinopyrum ponticum and gene Lr35 from Aegilops speltoides into hexaploid wheat by use of dominant STS and SCAR markers. Near isogenic lines with these genes are used in gene pyramiding to develop a single line with all three genes. By use of protein markers we develop near isogenic wheat lines for higher sedimentation values, higher dough strength and better breadmaking quality. These are lines with new combination of HMW glutenin subunits (21*, 7+8, 5+10) as well as wheat lines with new unknown HMW-GS and with new HMW-GS pair.
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spelling doaj.art-c1f9b8f0f8424a0a9e214db750db37482022-12-22T03:16:56ZengFaculty of Agrobitechnical Sciences OsijekPoljoprivreda1330-71421848-80802008-09-01141supMARKER ASSISTED SELECTION (MAS) FOR DEVELOPMENT OF BARLEY AND WHEAT LINES WITH REQUESTED TRAITSM. Hudcovicová0S. Šliková1E. Gregová2D. Mihálik3S. Gavurníková4Z. Šramková5Slovak Agricultural Research Center – Research Institute of Plant Production Piešťany, Bratislavská cesta 122, SK-921 68, Piešťany, Slovak RepublicSlovak Agricultural Research Center – Research Institute of Plant Production Piešťany, Bratislavská cesta 122, SK-921 68, Piešťany, Slovak RepublicSlovak Agricultural Research Center – Research Institute of Plant Production Piešťany, Bratislavská cesta 122, SK-921 68, Piešťany, Slovak RepublicSlovak Agricultural Research Center – Research Institute of Plant Production Piešťany, Bratislavská cesta 122, SK-921 68, Piešťany, Slovak RepublicSlovak Agricultural Research Center – Research Institute of Plant Production Piešťany, Bratislavská cesta 122, SK-921 68, Piešťany, Slovak RepublicSlovak Agricultural Research Center – Research Institute of Plant Production Piešťany, Bratislavská cesta 122, SK-921 68, Piešťany, Slovak RepublicMolecular markers closely linked to interesting genes enable early, proper and fast detection of plant individuals with desired allele during backcross breeding, what can make plant breeding faster and cheaper. We are focused on molecular breeding of barley and wheat lines for disease resistance and some important quality traits. As acceptors of interesting genes we use especially elite Slovak and Czech cultivars and lines. After five backcross generations with the help of MAS new created lines carrying markers linked to desired genes undergo resistance, agronomic and technological tests. In breeding of winter barley for resistance to BaYMV/BaMMV viruses we use codominant STS and SSR markers linked to rym4 and rym11 resistance genes. Cultivar Romanze has been used as a donor of rym4 gene and landrace Russia57 as gene rym11 donor. In spring barley we are focused on transfer of Yd2 gene from landraces Shannon and Sutter resistant to BYDV by use of dominant ASPCR marker. We are also working on transfer of effective leaf rust resistance genes Lr19, Lr24 derived from Thinopyrum ponticum and gene Lr35 from Aegilops speltoides into hexaploid wheat by use of dominant STS and SCAR markers. Near isogenic lines with these genes are used in gene pyramiding to develop a single line with all three genes. By use of protein markers we develop near isogenic wheat lines for higher sedimentation values, higher dough strength and better breadmaking quality. These are lines with new combination of HMW glutenin subunits (21*, 7+8, 5+10) as well as wheat lines with new unknown HMW-GS and with new HMW-GS pair.http://poljoprivreda.pfos.hr/upload/publications/poljoprivreda-14-1-sup17.pdf
spellingShingle M. Hudcovicová
S. Šliková
E. Gregová
D. Mihálik
S. Gavurníková
Z. Šramková
MARKER ASSISTED SELECTION (MAS) FOR DEVELOPMENT OF BARLEY AND WHEAT LINES WITH REQUESTED TRAITS
Poljoprivreda
title MARKER ASSISTED SELECTION (MAS) FOR DEVELOPMENT OF BARLEY AND WHEAT LINES WITH REQUESTED TRAITS
title_full MARKER ASSISTED SELECTION (MAS) FOR DEVELOPMENT OF BARLEY AND WHEAT LINES WITH REQUESTED TRAITS
title_fullStr MARKER ASSISTED SELECTION (MAS) FOR DEVELOPMENT OF BARLEY AND WHEAT LINES WITH REQUESTED TRAITS
title_full_unstemmed MARKER ASSISTED SELECTION (MAS) FOR DEVELOPMENT OF BARLEY AND WHEAT LINES WITH REQUESTED TRAITS
title_short MARKER ASSISTED SELECTION (MAS) FOR DEVELOPMENT OF BARLEY AND WHEAT LINES WITH REQUESTED TRAITS
title_sort marker assisted selection mas for development of barley and wheat lines with requested traits
url http://poljoprivreda.pfos.hr/upload/publications/poljoprivreda-14-1-sup17.pdf
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AT sslikova markerassistedselectionmasfordevelopmentofbarleyandwheatlineswithrequestedtraits
AT egregova markerassistedselectionmasfordevelopmentofbarleyandwheatlineswithrequestedtraits
AT dmihalik markerassistedselectionmasfordevelopmentofbarleyandwheatlineswithrequestedtraits
AT sgavurnikova markerassistedselectionmasfordevelopmentofbarleyandwheatlineswithrequestedtraits
AT zsramkova markerassistedselectionmasfordevelopmentofbarleyandwheatlineswithrequestedtraits