Design of Chlamydia Trachomatis Diagnostic Kit in Symptomatic Women Using Real Time PCR
Background & Objective: Chlamydia trachomatis is one of the most common sexually transmitted diseases worldwide. Screening women for Chlamydia trachomatis in developing countries is highly desirable due to the ineffectiveness of the available diagnostic methods. The aim of this study is to desig...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Fasa University of Medical Sciences
2020-04-01
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| Series: | Journal of Advanced Biomedical Sciences |
| Subjects: | |
| Online Access: | http://jabs.fums.ac.ir/article-1-2063-en.pdf |
| _version_ | 1797679582101372928 |
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| author | hamideh rouhani nejad negar rahmati fahimeh nemati mansor darya amiri |
| author_facet | hamideh rouhani nejad negar rahmati fahimeh nemati mansor darya amiri |
| author_sort | hamideh rouhani nejad |
| collection | DOAJ |
| description | Background & Objective: Chlamydia trachomatis is one of the most common sexually transmitted diseases worldwide. Screening women for Chlamydia trachomatis in developing countries is highly desirable due to the ineffectiveness of the available diagnostic methods. The aim of this study is to design a detection kit for Chlamydia trachomatis in symptomatic women.
Materials & Methods: A total of 50 clinical specimens of symptomatic women were collected. The OMP1 gene sequence was extracted from the gene bank and the primer and probe were designed appropriately by Mega6 software. The extracted DNA was amplified by PCR. In order to provide positive control, PCR product was cloned into Pjet1.2 vector. To determine the sensitivity, DNA dilution was performed. To determine the specificity, DNA of vaginal bacteria was extracted and Real Time PCR was done.
Results: In this study, we designed a kits with 100% specificity and 10pg/ϥl sensitivity in detecting chlamydial infection in symptomatic women.
Conclusion: With the use of designed primers and real time, we can detect 100% of chlamydia infections. |
| first_indexed | 2024-03-11T23:16:53Z |
| format | Article |
| id | doaj.art-c1fbf589d2984fa88bdc15ae288f3e65 |
| institution | Directory Open Access Journal |
| issn | 2228-5105 2783-1523 |
| language | English |
| last_indexed | 2024-03-11T23:16:53Z |
| publishDate | 2020-04-01 |
| publisher | Fasa University of Medical Sciences |
| record_format | Article |
| series | Journal of Advanced Biomedical Sciences |
| spelling | doaj.art-c1fbf589d2984fa88bdc15ae288f3e652023-09-20T21:27:06ZengFasa University of Medical SciencesJournal of Advanced Biomedical Sciences2228-51052783-15232020-04-0110121032110Design of Chlamydia Trachomatis Diagnostic Kit in Symptomatic Women Using Real Time PCRhamideh rouhani nejad0negar rahmati1fahimeh nemati mansor2darya amiri3 Malek-Ashtar University of Technology, Tehran, Iran Department of Microbioology, Pharmaceutical Sciences Branch, Islamic Azad University, Tehran, Iran 3. Department of biotechnology, Faculty of Science and Technologies, Pharmaceutical Sciences Branch, Islamic Azad University, Tehran, Iran Malek-Ashtar University of Technology, Tehran, Iran Background & Objective: Chlamydia trachomatis is one of the most common sexually transmitted diseases worldwide. Screening women for Chlamydia trachomatis in developing countries is highly desirable due to the ineffectiveness of the available diagnostic methods. The aim of this study is to design a detection kit for Chlamydia trachomatis in symptomatic women. Materials & Methods: A total of 50 clinical specimens of symptomatic women were collected. The OMP1 gene sequence was extracted from the gene bank and the primer and probe were designed appropriately by Mega6 software. The extracted DNA was amplified by PCR. In order to provide positive control, PCR product was cloned into Pjet1.2 vector. To determine the sensitivity, DNA dilution was performed. To determine the specificity, DNA of vaginal bacteria was extracted and Real Time PCR was done. Results: In this study, we designed a kits with 100% specificity and 10pg/ϥl sensitivity in detecting chlamydial infection in symptomatic women. Conclusion: With the use of designed primers and real time, we can detect 100% of chlamydia infections.http://jabs.fums.ac.ir/article-1-2063-en.pdfreal time pcrchlamydia trachomatisomp1 gene |
| spellingShingle | hamideh rouhani nejad negar rahmati fahimeh nemati mansor darya amiri Design of Chlamydia Trachomatis Diagnostic Kit in Symptomatic Women Using Real Time PCR Journal of Advanced Biomedical Sciences real time pcr chlamydia trachomatis omp1 gene |
| title | Design of Chlamydia Trachomatis Diagnostic Kit in Symptomatic Women Using Real Time PCR |
| title_full | Design of Chlamydia Trachomatis Diagnostic Kit in Symptomatic Women Using Real Time PCR |
| title_fullStr | Design of Chlamydia Trachomatis Diagnostic Kit in Symptomatic Women Using Real Time PCR |
| title_full_unstemmed | Design of Chlamydia Trachomatis Diagnostic Kit in Symptomatic Women Using Real Time PCR |
| title_short | Design of Chlamydia Trachomatis Diagnostic Kit in Symptomatic Women Using Real Time PCR |
| title_sort | design of chlamydia trachomatis diagnostic kit in symptomatic women using real time pcr |
| topic | real time pcr chlamydia trachomatis omp1 gene |
| url | http://jabs.fums.ac.ir/article-1-2063-en.pdf |
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