Striking Differences in Platelet Distribution between Advanced-Platelet-Rich Fibrin and Concentrated Growth Factors: Effects of Silica-Containing Plastic Tubes
Compared with platelet-rich plasma, the preparation of platelet-rich fibrin (PRF) is simple and has not been overly modified. However, it was recently demonstrated that centrifugation conditions influence the composition of PRF and that silica microparticles from silica-coated plastic tubes can ente...
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-09-01
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| Series: | Journal of Functional Biomaterials |
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| Online Access: | https://www.mdpi.com/2079-4983/10/3/43 |
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| author | Tetsuhiro Tsujino Hideo Masuki Masayuki Nakamura Kazushige Isobe Hideo Kawabata Hachidai Aizawa Taisuke Watanabe Yutaka Kitamura Hajime Okudera Kazuhiro Okuda Koh Nakata Tomoyuki Kawase |
| author_facet | Tetsuhiro Tsujino Hideo Masuki Masayuki Nakamura Kazushige Isobe Hideo Kawabata Hachidai Aizawa Taisuke Watanabe Yutaka Kitamura Hajime Okudera Kazuhiro Okuda Koh Nakata Tomoyuki Kawase |
| author_sort | Tetsuhiro Tsujino |
| collection | DOAJ |
| description | Compared with platelet-rich plasma, the preparation of platelet-rich fibrin (PRF) is simple and has not been overly modified. However, it was recently demonstrated that centrifugation conditions influence the composition of PRF and that silica microparticles from silica-coated plastic tubes can enter the PRF matrix. These factors may also modify platelet distribution. To examine these possibilities, we prepared PRF matrices using various types of blood-collection tubes (plain glass tubes and silica-containing plastic tubes) and different centrifugation speeds. The protocols of concentrated growth factors and advanced-PRF represented high- and low-speed centrifugation, respectively. Platelet distribution in the PRF matrix was examined immunohistochemically. Using low-speed centrifugation, platelets were distributed homogeneously within the PRF matrix regardless of tube types. In high-speed centrifugation, platelets were distributed mainly on one surface region of the PRF matrix in glass tubes, whereas in silica-coated tubes, platelet distribution was commonly more diffusive than in glass tubes. Therefore, both blood-collection tube types and centrifugal conditions appeared to influence platelet distribution in the PRF matrix. Platelets distributed in the deep regions of the PRF matrix may contribute to better growth factor retention and release. However, clinicians should be careful in using silica-coated tubes because their silica microparticles may be a health hazard. |
| first_indexed | 2024-12-10T06:53:10Z |
| format | Article |
| id | doaj.art-c1fe2a20c96342a5ac424bec7166fc0f |
| institution | Directory Open Access Journal |
| issn | 2079-4983 |
| language | English |
| last_indexed | 2024-12-10T06:53:10Z |
| publishDate | 2019-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Functional Biomaterials |
| spelling | doaj.art-c1fe2a20c96342a5ac424bec7166fc0f2022-12-22T01:58:30ZengMDPI AGJournal of Functional Biomaterials2079-49832019-09-011034310.3390/jfb10030043jfb10030043Striking Differences in Platelet Distribution between Advanced-Platelet-Rich Fibrin and Concentrated Growth Factors: Effects of Silica-Containing Plastic TubesTetsuhiro Tsujino0Hideo Masuki1Masayuki Nakamura2Kazushige Isobe3Hideo Kawabata4Hachidai Aizawa5Taisuke Watanabe6Yutaka Kitamura7Hajime Okudera8Kazuhiro Okuda9Koh Nakata10Tomoyuki Kawase11Tokyo Plastic Dental Society, Kita-ku, Tokyo 114-0002, JapanTokyo Plastic Dental Society, Kita-ku, Tokyo 114-0002, JapanTokyo Plastic Dental Society, Kita-ku, Tokyo 114-0002, JapanTokyo Plastic Dental Society, Kita-ku, Tokyo 114-0002, JapanTokyo Plastic Dental Society, Kita-ku, Tokyo 114-0002, JapanTokyo Plastic Dental Society, Kita-ku, Tokyo 114-0002, JapanTokyo Plastic Dental Society, Kita-ku, Tokyo 114-0002, JapanTokyo Plastic Dental Society, Kita-ku, Tokyo 114-0002, JapanTokyo Plastic Dental Society, Kita-ku, Tokyo 114-0002, JapanDivision of Periodontology, Institute of Medicine and Dentistry, Niigata University, Niigata 951-8514, JapanBioscience Medical Research Center, Niigata University Medical and Dental Hospital, Niigata 951-8520, JapanDivision of Oral Bioengineering, Institute of Medicine and Dentistry, Niigata University, Niigata 951-8514, JapanCompared with platelet-rich plasma, the preparation of platelet-rich fibrin (PRF) is simple and has not been overly modified. However, it was recently demonstrated that centrifugation conditions influence the composition of PRF and that silica microparticles from silica-coated plastic tubes can enter the PRF matrix. These factors may also modify platelet distribution. To examine these possibilities, we prepared PRF matrices using various types of blood-collection tubes (plain glass tubes and silica-containing plastic tubes) and different centrifugation speeds. The protocols of concentrated growth factors and advanced-PRF represented high- and low-speed centrifugation, respectively. Platelet distribution in the PRF matrix was examined immunohistochemically. Using low-speed centrifugation, platelets were distributed homogeneously within the PRF matrix regardless of tube types. In high-speed centrifugation, platelets were distributed mainly on one surface region of the PRF matrix in glass tubes, whereas in silica-coated tubes, platelet distribution was commonly more diffusive than in glass tubes. Therefore, both blood-collection tube types and centrifugal conditions appeared to influence platelet distribution in the PRF matrix. Platelets distributed in the deep regions of the PRF matrix may contribute to better growth factor retention and release. However, clinicians should be careful in using silica-coated tubes because their silica microparticles may be a health hazard.https://www.mdpi.com/2079-4983/10/3/43advanced platelet-rich fibrinconcentrated growth factorsplateletsCD41centrifugal forcesilica |
| spellingShingle | Tetsuhiro Tsujino Hideo Masuki Masayuki Nakamura Kazushige Isobe Hideo Kawabata Hachidai Aizawa Taisuke Watanabe Yutaka Kitamura Hajime Okudera Kazuhiro Okuda Koh Nakata Tomoyuki Kawase Striking Differences in Platelet Distribution between Advanced-Platelet-Rich Fibrin and Concentrated Growth Factors: Effects of Silica-Containing Plastic Tubes Journal of Functional Biomaterials advanced platelet-rich fibrin concentrated growth factors platelets CD41 centrifugal force silica |
| title | Striking Differences in Platelet Distribution between Advanced-Platelet-Rich Fibrin and Concentrated Growth Factors: Effects of Silica-Containing Plastic Tubes |
| title_full | Striking Differences in Platelet Distribution between Advanced-Platelet-Rich Fibrin and Concentrated Growth Factors: Effects of Silica-Containing Plastic Tubes |
| title_fullStr | Striking Differences in Platelet Distribution between Advanced-Platelet-Rich Fibrin and Concentrated Growth Factors: Effects of Silica-Containing Plastic Tubes |
| title_full_unstemmed | Striking Differences in Platelet Distribution between Advanced-Platelet-Rich Fibrin and Concentrated Growth Factors: Effects of Silica-Containing Plastic Tubes |
| title_short | Striking Differences in Platelet Distribution between Advanced-Platelet-Rich Fibrin and Concentrated Growth Factors: Effects of Silica-Containing Plastic Tubes |
| title_sort | striking differences in platelet distribution between advanced platelet rich fibrin and concentrated growth factors effects of silica containing plastic tubes |
| topic | advanced platelet-rich fibrin concentrated growth factors platelets CD41 centrifugal force silica |
| url | https://www.mdpi.com/2079-4983/10/3/43 |
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