Microbiome overview in swine lungs.
Mycoplasma hyopneumoniae is the etiologic agent of swine enzootic pneumonia. However other mycoplasma species and secondary bacteria are found as inhabitants of the swine respiratory tract, which can be also related to disease. In the present study we have performed a total DNA metagenomic analysis...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2017-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC5515459?pdf=render |
| _version_ | 1818616063902875648 |
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| author | Franciele Maboni Siqueira Esteban Pérez-Wohlfeil Fabíola Marques Carvalho Oswaldo Trelles Irene Silveira Schrank Ana Tereza Ribeiro Vasconcelos Arnaldo Zaha |
| author_facet | Franciele Maboni Siqueira Esteban Pérez-Wohlfeil Fabíola Marques Carvalho Oswaldo Trelles Irene Silveira Schrank Ana Tereza Ribeiro Vasconcelos Arnaldo Zaha |
| author_sort | Franciele Maboni Siqueira |
| collection | DOAJ |
| description | Mycoplasma hyopneumoniae is the etiologic agent of swine enzootic pneumonia. However other mycoplasma species and secondary bacteria are found as inhabitants of the swine respiratory tract, which can be also related to disease. In the present study we have performed a total DNA metagenomic analysis from the lungs of pigs kept in a field condition, with suggestive signals of enzootic pneumonia and without any infection signals to evaluate the bacteria variability of the lungs microbiota. Libraries from metagenomic DNA were prepared and sequenced using total DNA shotgun metagenomic pyrosequencing. The metagenomic distribution showed a great abundance of bacteria. The most common microbial families identified from pneumonic swine's lungs were Mycoplasmataceae, Flavobacteriaceae and Pasteurellaceae, whereas in the carrier swine's lungs the most common families were Mycoplasmataceae, Bradyrhizobiaceae and Flavobacteriaceae. Analysis of community composition in both samples confirmed the high prevalence of M. hyopneumoniae. Moreover, the carrier lungs had more diverse family population, which should be related to the lungs normal flora. In summary, we provide a wide view of the bacterial population from lungs with signals of enzootic pneumonia and lungs without signals of enzootic pneumonia in a field situation. These bacteria patterns provide information that may be important for the establishment of disease control measures and to give insights for further studies. |
| first_indexed | 2024-12-16T16:43:51Z |
| format | Article |
| id | doaj.art-c200701ebd1541e2a17698bee7e9ae0b |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-16T16:43:51Z |
| publishDate | 2017-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-c200701ebd1541e2a17698bee7e9ae0b2022-12-21T22:24:14ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-01127e018150310.1371/journal.pone.0181503Microbiome overview in swine lungs.Franciele Maboni SiqueiraEsteban Pérez-WohlfeilFabíola Marques CarvalhoOswaldo TrellesIrene Silveira SchrankAna Tereza Ribeiro VasconcelosArnaldo ZahaMycoplasma hyopneumoniae is the etiologic agent of swine enzootic pneumonia. However other mycoplasma species and secondary bacteria are found as inhabitants of the swine respiratory tract, which can be also related to disease. In the present study we have performed a total DNA metagenomic analysis from the lungs of pigs kept in a field condition, with suggestive signals of enzootic pneumonia and without any infection signals to evaluate the bacteria variability of the lungs microbiota. Libraries from metagenomic DNA were prepared and sequenced using total DNA shotgun metagenomic pyrosequencing. The metagenomic distribution showed a great abundance of bacteria. The most common microbial families identified from pneumonic swine's lungs were Mycoplasmataceae, Flavobacteriaceae and Pasteurellaceae, whereas in the carrier swine's lungs the most common families were Mycoplasmataceae, Bradyrhizobiaceae and Flavobacteriaceae. Analysis of community composition in both samples confirmed the high prevalence of M. hyopneumoniae. Moreover, the carrier lungs had more diverse family population, which should be related to the lungs normal flora. In summary, we provide a wide view of the bacterial population from lungs with signals of enzootic pneumonia and lungs without signals of enzootic pneumonia in a field situation. These bacteria patterns provide information that may be important for the establishment of disease control measures and to give insights for further studies.http://europepmc.org/articles/PMC5515459?pdf=render |
| spellingShingle | Franciele Maboni Siqueira Esteban Pérez-Wohlfeil Fabíola Marques Carvalho Oswaldo Trelles Irene Silveira Schrank Ana Tereza Ribeiro Vasconcelos Arnaldo Zaha Microbiome overview in swine lungs. PLoS ONE |
| title | Microbiome overview in swine lungs. |
| title_full | Microbiome overview in swine lungs. |
| title_fullStr | Microbiome overview in swine lungs. |
| title_full_unstemmed | Microbiome overview in swine lungs. |
| title_short | Microbiome overview in swine lungs. |
| title_sort | microbiome overview in swine lungs |
| url | http://europepmc.org/articles/PMC5515459?pdf=render |
| work_keys_str_mv | AT francielemabonisiqueira microbiomeoverviewinswinelungs AT estebanperezwohlfeil microbiomeoverviewinswinelungs AT fabiolamarquescarvalho microbiomeoverviewinswinelungs AT oswaldotrelles microbiomeoverviewinswinelungs AT irenesilveiraschrank microbiomeoverviewinswinelungs AT anaterezaribeirovasconcelos microbiomeoverviewinswinelungs AT arnaldozaha microbiomeoverviewinswinelungs |