Effect of Peptides on the Synthesis, Properties and Wound Healing Capacity of Silver Nanoparticles
The aim of this study is the synthesis of novel peptide–silver nanoparticle conjugates with enhanced wound healing capacity. Peptide–silver nanoparticle conjugates were synthesized using myristoyl tetrapeptide 6 (MT6) or copper tripeptide 1 (CuTP1). Peptide-free silver nanoparticles (AgNP) were synt...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-10-01
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| Series: | Pharmaceutics |
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| Online Access: | https://www.mdpi.com/1999-4923/15/10/2471 |
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| author | Afroditi Papaioannou Angeliki Liakopoulou Dimitris Papoulis Eleni Gianni Patroula Gkolfi Eleni Zygouri Sophia Letsiou Sophia Hatziantoniou |
| author_facet | Afroditi Papaioannou Angeliki Liakopoulou Dimitris Papoulis Eleni Gianni Patroula Gkolfi Eleni Zygouri Sophia Letsiou Sophia Hatziantoniou |
| author_sort | Afroditi Papaioannou |
| collection | DOAJ |
| description | The aim of this study is the synthesis of novel peptide–silver nanoparticle conjugates with enhanced wound healing capacity. Peptide–silver nanoparticle conjugates were synthesized using myristoyl tetrapeptide 6 (MT6) or copper tripeptide 1 (CuTP1). Peptide-free silver nanoparticles (AgNP) were synthesized using NaBH4 and sodium citrate and were used as control. The addition of the peptides during or after the synthesis of nanoparticles and its impact on the properties of the synthesized peptide–silver nanoparticle conjugates were assessed. The monitoring of the synthesis of nanoparticles was achieved using ultraviolet–visible spectrophotometry (UV–/Vis). The characteristics and colloidal stability of the nanoparticles (size and ζ-potential distribution, morphology, composition and structure) were monitored using dynamic laser scattering (DLS), transmission electron microscopy (TEM), atomic absorption spectroscopy (AAS) and X-ray diffraction (XRD). The wound healing capacity of the peptide–silver nanoparticle conjugates was assessed using scratch test assay on fibroblasts (NIH/3T3). The results indicated that the addition of the peptides during the synthesis of nanoparticles lead to better yield of the reaction and more effective capping while the size distribution and ζ-potential of the conjugates indicated long-term colloidal stability. The MT6-AgNP conjugate exhibited 71.97 ± 4.35% wound closure, which was about 5.48-fold higher (<i>p</i> < 0.05) than the corresponding free MT6. The CuTP1-AgNP conjugate exhibited 62.37 ± 18.33% wound closure that was better by 2.82 fold (<i>p</i> < 0.05) compared to the corresponding free CuTP1. Both peptides led to the synthesis of silver nanoparticle conjugates with enhanced wound healing capacity compared to the respective free peptide or to the peptide-free AgNP (29.53 ± 4.71% wound closure, <i>p</i> < 0.05). Our findings demonstrated that the synthetized peptide–silver nanoparticle conjugates are promising ingredients for wound care formulation. |
| first_indexed | 2024-03-10T20:57:59Z |
| format | Article |
| id | doaj.art-c2023e3696e54363bc20b82792e2d2db |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-10T20:57:59Z |
| publishDate | 2023-10-01 |
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| series | Pharmaceutics |
| spelling | doaj.art-c2023e3696e54363bc20b82792e2d2db2023-11-19T17:45:00ZengMDPI AGPharmaceutics1999-49232023-10-011510247110.3390/pharmaceutics15102471Effect of Peptides on the Synthesis, Properties and Wound Healing Capacity of Silver NanoparticlesAfroditi Papaioannou0Angeliki Liakopoulou1Dimitris Papoulis2Eleni Gianni3Patroula Gkolfi4Eleni Zygouri5Sophia Letsiou6Sophia Hatziantoniou7Department of Pharmacy, University of Patras, 26504 Patras, GreeceDepartment of Pharmacy, University of Patras, 26504 Patras, GreeceDepartment of Geology, University of Patras, 26504 Patras, GreeceDepartment of Geology, University of Patras, 26504 Patras, GreeceDepartment of Chemistry, University of Patras, 26504 Patras, GreeceDepartment of Chemistry, University of Patras, 26504 Patras, GreeceDepartment of Food Science and Technology, University of West Attica, Agiou Spyridonos 28, 12243 Aegaleo, GreeceDepartment of Pharmacy, University of Patras, 26504 Patras, GreeceThe aim of this study is the synthesis of novel peptide–silver nanoparticle conjugates with enhanced wound healing capacity. Peptide–silver nanoparticle conjugates were synthesized using myristoyl tetrapeptide 6 (MT6) or copper tripeptide 1 (CuTP1). Peptide-free silver nanoparticles (AgNP) were synthesized using NaBH4 and sodium citrate and were used as control. The addition of the peptides during or after the synthesis of nanoparticles and its impact on the properties of the synthesized peptide–silver nanoparticle conjugates were assessed. The monitoring of the synthesis of nanoparticles was achieved using ultraviolet–visible spectrophotometry (UV–/Vis). The characteristics and colloidal stability of the nanoparticles (size and ζ-potential distribution, morphology, composition and structure) were monitored using dynamic laser scattering (DLS), transmission electron microscopy (TEM), atomic absorption spectroscopy (AAS) and X-ray diffraction (XRD). The wound healing capacity of the peptide–silver nanoparticle conjugates was assessed using scratch test assay on fibroblasts (NIH/3T3). The results indicated that the addition of the peptides during the synthesis of nanoparticles lead to better yield of the reaction and more effective capping while the size distribution and ζ-potential of the conjugates indicated long-term colloidal stability. The MT6-AgNP conjugate exhibited 71.97 ± 4.35% wound closure, which was about 5.48-fold higher (<i>p</i> < 0.05) than the corresponding free MT6. The CuTP1-AgNP conjugate exhibited 62.37 ± 18.33% wound closure that was better by 2.82 fold (<i>p</i> < 0.05) compared to the corresponding free CuTP1. Both peptides led to the synthesis of silver nanoparticle conjugates with enhanced wound healing capacity compared to the respective free peptide or to the peptide-free AgNP (29.53 ± 4.71% wound closure, <i>p</i> < 0.05). Our findings demonstrated that the synthetized peptide–silver nanoparticle conjugates are promising ingredients for wound care formulation.https://www.mdpi.com/1999-4923/15/10/2471AgNPsilver nanoparticlespeptide conjugateswound healingscratch testmyristoyl tetrapeptide 6 |
| spellingShingle | Afroditi Papaioannou Angeliki Liakopoulou Dimitris Papoulis Eleni Gianni Patroula Gkolfi Eleni Zygouri Sophia Letsiou Sophia Hatziantoniou Effect of Peptides on the Synthesis, Properties and Wound Healing Capacity of Silver Nanoparticles Pharmaceutics AgNP silver nanoparticles peptide conjugates wound healing scratch test myristoyl tetrapeptide 6 |
| title | Effect of Peptides on the Synthesis, Properties and Wound Healing Capacity of Silver Nanoparticles |
| title_full | Effect of Peptides on the Synthesis, Properties and Wound Healing Capacity of Silver Nanoparticles |
| title_fullStr | Effect of Peptides on the Synthesis, Properties and Wound Healing Capacity of Silver Nanoparticles |
| title_full_unstemmed | Effect of Peptides on the Synthesis, Properties and Wound Healing Capacity of Silver Nanoparticles |
| title_short | Effect of Peptides on the Synthesis, Properties and Wound Healing Capacity of Silver Nanoparticles |
| title_sort | effect of peptides on the synthesis properties and wound healing capacity of silver nanoparticles |
| topic | AgNP silver nanoparticles peptide conjugates wound healing scratch test myristoyl tetrapeptide 6 |
| url | https://www.mdpi.com/1999-4923/15/10/2471 |
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