Saccharide sources do not influence the biofilm formation in Scedosporium/Lomentospora species
Scedosporium and Lomentospora species are ubiquitous saprophytic filamentous fungi that emerged as human pathogens with impressive multidrug-resistance profile. The ability to form biofilm over several biotic and abiotic surfaces is one of the characteristics that contributes to their resistance pat...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Cambridge University Press
2020-01-01
|
| Series: | Experimental Results |
| Subjects: | |
| Online Access: | https://www.cambridge.org/core/product/identifier/S2516712X19000054/type/journal_article |
| _version_ | 1811156257385480192 |
|---|---|
| author | Thaís Pereira de Mello Marta Helena Branquinha André Luis Souza dos Santos Michael Nevels |
| author_facet | Thaís Pereira de Mello Marta Helena Branquinha André Luis Souza dos Santos Michael Nevels |
| author_sort | Thaís Pereira de Mello |
| collection | DOAJ |
| description | Scedosporium and Lomentospora species are ubiquitous saprophytic filamentous fungi that emerged as human pathogens with impressive multidrug-resistance profile. The ability to form biofilm over several biotic and abiotic surfaces is one of the characteristics that contributes to their resistance patterns against almost all currently available antifungals. Herein, we have demonstrated that Scedosporium apiospermum, Scedosporium minutisporum, Scedosporium aurantiacum and Lomentospora prolificans were able to form biofilm, in similar amounts, when conidial cells were incubated in a polystyrene substrate containing Sabouraud medium supplemented or not with different concentrations (2%, 5% and 10%) of glucose, fructose, sucrose and lactose. Likewise, the glucose supplementation of culture media primarily composed of amino acids (SCFM, synthetic cystic fibrosis medium) and salts (YNB, yeast nitrogen base) did not modulate the biofilm formation of Scedosporium/Lomentospora species. Collectively, the present data reinforce the ability of these opportunistic fungi to colonize and to build biofilm structures under different environmental conditions. |
| first_indexed | 2024-04-10T04:48:37Z |
| format | Article |
| id | doaj.art-c2059c7a6acd480da2356a35e6693b90 |
| institution | Directory Open Access Journal |
| issn | 2516-712X |
| language | English |
| last_indexed | 2024-04-10T04:48:37Z |
| publishDate | 2020-01-01 |
| publisher | Cambridge University Press |
| record_format | Article |
| series | Experimental Results |
| spelling | doaj.art-c2059c7a6acd480da2356a35e6693b902023-03-09T12:34:21ZengCambridge University PressExperimental Results2516-712X2020-01-01110.1017/exp.2019.5Saccharide sources do not influence the biofilm formation in Scedosporium/Lomentospora speciesThaís Pereira de Mello0Marta Helena Branquinha1André Luis Souza dos Santos2https://orcid.org/0000-0003-0821-8592Michael Nevels3Laboratório de Estudos Avançados de Microrganismos Emergentes e Resistentes, Departamento de Microbiologia Geral, Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, BrazilPrograma de Pós-Graduação em Bioquímica, Instituto de Química, UFRJ, Rio de Janeiro, BrazilLaboratório de Estudos Avançados de Microrganismos Emergentes e Resistentes, Departamento de Microbiologia Geral, Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil Programa de Pós-Graduação em Bioquímica, Instituto de Química, UFRJ, Rio de Janeiro, BrazilUniversity of St Andrews, Biomolecular Sciences Building, Fife, United Kingdom of Great Britain and Northern Ireland, KY16 9STScedosporium and Lomentospora species are ubiquitous saprophytic filamentous fungi that emerged as human pathogens with impressive multidrug-resistance profile. The ability to form biofilm over several biotic and abiotic surfaces is one of the characteristics that contributes to their resistance patterns against almost all currently available antifungals. Herein, we have demonstrated that Scedosporium apiospermum, Scedosporium minutisporum, Scedosporium aurantiacum and Lomentospora prolificans were able to form biofilm, in similar amounts, when conidial cells were incubated in a polystyrene substrate containing Sabouraud medium supplemented or not with different concentrations (2%, 5% and 10%) of glucose, fructose, sucrose and lactose. Likewise, the glucose supplementation of culture media primarily composed of amino acids (SCFM, synthetic cystic fibrosis medium) and salts (YNB, yeast nitrogen base) did not modulate the biofilm formation of Scedosporium/Lomentospora species. Collectively, the present data reinforce the ability of these opportunistic fungi to colonize and to build biofilm structures under different environmental conditions.https://www.cambridge.org/core/product/identifier/S2516712X19000054/type/journal_articleScedosporium/LomentosporaFilamentous fungiBiofilmSaccharidesGrowth conditions |
| spellingShingle | Thaís Pereira de Mello Marta Helena Branquinha André Luis Souza dos Santos Michael Nevels Saccharide sources do not influence the biofilm formation in Scedosporium/Lomentospora species Experimental Results Scedosporium/Lomentospora Filamentous fungi Biofilm Saccharides Growth conditions |
| title | Saccharide sources do not influence the biofilm formation in Scedosporium/Lomentospora species |
| title_full | Saccharide sources do not influence the biofilm formation in Scedosporium/Lomentospora species |
| title_fullStr | Saccharide sources do not influence the biofilm formation in Scedosporium/Lomentospora species |
| title_full_unstemmed | Saccharide sources do not influence the biofilm formation in Scedosporium/Lomentospora species |
| title_short | Saccharide sources do not influence the biofilm formation in Scedosporium/Lomentospora species |
| title_sort | saccharide sources do not influence the biofilm formation in scedosporium lomentospora species |
| topic | Scedosporium/Lomentospora Filamentous fungi Biofilm Saccharides Growth conditions |
| url | https://www.cambridge.org/core/product/identifier/S2516712X19000054/type/journal_article |
| work_keys_str_mv | AT thaispereirademello saccharidesourcesdonotinfluencethebiofilmformationinscedosporiumlomentosporaspecies AT martahelenabranquinha saccharidesourcesdonotinfluencethebiofilmformationinscedosporiumlomentosporaspecies AT andreluissouzadossantos saccharidesourcesdonotinfluencethebiofilmformationinscedosporiumlomentosporaspecies AT michaelnevels saccharidesourcesdonotinfluencethebiofilmformationinscedosporiumlomentosporaspecies |