<it>In planta </it>production of two peptides of the Classical Swine Fever Virus (CSFV) E2 glycoprotein fused to the coat protein of potato virus X
<p>Abstract</p> <p>Background</p> <p>Classical Swine Fever (CSFV) is one of the most important viral infectious diseases affecting wild boars and domestic pigs. The etiological agent of the disease is the CSF virus, a single stranded RNA virus belonging to the family &l...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2006-06-01
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| Series: | BMC Biotechnology |
| Online Access: | http://www.biomedcentral.com/1472-6750/6/29 |
| _version_ | 1811314946981494784 |
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| author | Lico Chiara De Marchis Francesca Barone Pierluigi Albertini Emidio Marconi Gianpiero Marusic Carla Rutili Domenico Veronesi Fabio Porceddu Andrea |
| author_facet | Lico Chiara De Marchis Francesca Barone Pierluigi Albertini Emidio Marconi Gianpiero Marusic Carla Rutili Domenico Veronesi Fabio Porceddu Andrea |
| author_sort | Lico Chiara |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Classical Swine Fever (CSFV) is one of the most important viral infectious diseases affecting wild boars and domestic pigs. The etiological agent of the disease is the CSF virus, a single stranded RNA virus belonging to the family <it>Flaviviridae</it>.</p> <p>All preventive measures in domestic pigs have been focused in interrupting the chain of infection and in avoiding the spread of CSFV within wild boars as well as interrupting transmission from wild boars to domestic pigs. The use of plant based vaccine against CSFV would be advantageous as plant organs can be distributed without the need of particular treatments such as refrigeration and therefore large areas, populated by wild animals, could be easily covered.</p> <p>Results</p> <p>We report the <it>in planta </it>production of peptides of the classical swine fever (CSF) E2 glycoprotein fused to the coat protein of potato virus X. RT-PCR studies demonstrated that the peptide encoding sequences are correctly retained in the PVX construct after three sequential passage in <it>Nicotiana benthamiana </it>plants. Sequence analysis of RT-PCR products confirmed that the epitope coding sequences are replicated with high fidelity during PVX infection. Partially purified virions were able to induce an immune response in rabbits.</p> <p>Conclusion</p> <p>Previous reports have demonstrated that E2 synthetic peptides can efficiently induce an immunoprotective response in immunogenized animals. In this work we have showed that E2 peptides can be expressed <it>in planta </it>by using a modified PVX vector. These results are particularly promising for designing strategies for disease containment in areas inhabited by wild boars.</p> |
| first_indexed | 2024-04-13T11:20:15Z |
| format | Article |
| id | doaj.art-c20c647d6f5945b2a5036e10303c85d3 |
| institution | Directory Open Access Journal |
| issn | 1472-6750 |
| language | English |
| last_indexed | 2024-04-13T11:20:15Z |
| publishDate | 2006-06-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Biotechnology |
| spelling | doaj.art-c20c647d6f5945b2a5036e10303c85d32022-12-22T02:48:50ZengBMCBMC Biotechnology1472-67502006-06-01612910.1186/1472-6750-6-29<it>In planta </it>production of two peptides of the Classical Swine Fever Virus (CSFV) E2 glycoprotein fused to the coat protein of potato virus XLico ChiaraDe Marchis FrancescaBarone PierluigiAlbertini EmidioMarconi GianpieroMarusic CarlaRutili DomenicoVeronesi FabioPorceddu Andrea<p>Abstract</p> <p>Background</p> <p>Classical Swine Fever (CSFV) is one of the most important viral infectious diseases affecting wild boars and domestic pigs. The etiological agent of the disease is the CSF virus, a single stranded RNA virus belonging to the family <it>Flaviviridae</it>.</p> <p>All preventive measures in domestic pigs have been focused in interrupting the chain of infection and in avoiding the spread of CSFV within wild boars as well as interrupting transmission from wild boars to domestic pigs. The use of plant based vaccine against CSFV would be advantageous as plant organs can be distributed without the need of particular treatments such as refrigeration and therefore large areas, populated by wild animals, could be easily covered.</p> <p>Results</p> <p>We report the <it>in planta </it>production of peptides of the classical swine fever (CSF) E2 glycoprotein fused to the coat protein of potato virus X. RT-PCR studies demonstrated that the peptide encoding sequences are correctly retained in the PVX construct after three sequential passage in <it>Nicotiana benthamiana </it>plants. Sequence analysis of RT-PCR products confirmed that the epitope coding sequences are replicated with high fidelity during PVX infection. Partially purified virions were able to induce an immune response in rabbits.</p> <p>Conclusion</p> <p>Previous reports have demonstrated that E2 synthetic peptides can efficiently induce an immunoprotective response in immunogenized animals. In this work we have showed that E2 peptides can be expressed <it>in planta </it>by using a modified PVX vector. These results are particularly promising for designing strategies for disease containment in areas inhabited by wild boars.</p>http://www.biomedcentral.com/1472-6750/6/29 |
| spellingShingle | Lico Chiara De Marchis Francesca Barone Pierluigi Albertini Emidio Marconi Gianpiero Marusic Carla Rutili Domenico Veronesi Fabio Porceddu Andrea <it>In planta </it>production of two peptides of the Classical Swine Fever Virus (CSFV) E2 glycoprotein fused to the coat protein of potato virus X BMC Biotechnology |
| title | <it>In planta </it>production of two peptides of the Classical Swine Fever Virus (CSFV) E2 glycoprotein fused to the coat protein of potato virus X |
| title_full | <it>In planta </it>production of two peptides of the Classical Swine Fever Virus (CSFV) E2 glycoprotein fused to the coat protein of potato virus X |
| title_fullStr | <it>In planta </it>production of two peptides of the Classical Swine Fever Virus (CSFV) E2 glycoprotein fused to the coat protein of potato virus X |
| title_full_unstemmed | <it>In planta </it>production of two peptides of the Classical Swine Fever Virus (CSFV) E2 glycoprotein fused to the coat protein of potato virus X |
| title_short | <it>In planta </it>production of two peptides of the Classical Swine Fever Virus (CSFV) E2 glycoprotein fused to the coat protein of potato virus X |
| title_sort | it in planta it production of two peptides of the classical swine fever virus csfv e2 glycoprotein fused to the coat protein of potato virus x |
| url | http://www.biomedcentral.com/1472-6750/6/29 |
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