The Survey on -592 Polymorphism of Interlukin-10 in Hepatitis B Virus Infected Patients
Background: Infection with hepatitis B virus (HBV) may result in a number of different clinical outcomes. There are strong evidences in HBV infection that host genetic factors play a major role in determining the outcome of infection. Polymorphisms in the promoter region of interleukin 10 (IL-10) af...
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| Format: | Article |
| Language: | fas |
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Isfahan University of Medical Sciences
2014-03-01
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| Series: | مجله دانشکده پزشکی اصفهان |
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| Online Access: | http://jims.mui.ac.ir/index.php/jims/article/view/3230 |
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| author | Mohammad Hassan Bagheri-Mansoori Zohreh Sharifi Mohammad Hossein Sanati Abolhassan Shahzadeh-Fazeli Mansoureh Farhangnia |
| author_facet | Mohammad Hassan Bagheri-Mansoori Zohreh Sharifi Mohammad Hossein Sanati Abolhassan Shahzadeh-Fazeli Mansoureh Farhangnia |
| author_sort | Mohammad Hassan Bagheri-Mansoori |
| collection | DOAJ |
| description | Background: Infection with hepatitis B virus (HBV) may result in a number of different clinical outcomes. There are strong evidences in HBV infection that host genetic factors play a major role in determining the outcome of infection. Polymorphisms in the promoter region of interleukin 10 (IL-10) affect its production and confer susceptibility to inflammatory diseases. The aim of present study was to investigate the association between HBV infection and -592 polymorphism in the promoter region of the IL-10 gene in Iranian population.
Methods: 100 HBV infected patients and 100 healthy individuals were randomly selected. Genomic DNA was extracted from blood buffy coat using the salting-out method. The IL-10-592(A/C) genotypes were determined using allele-specific polymerase chain reaction (PCR) method. The PCR products were electrophoresed on 1.5% agarose gel. The data were analyzed using chi-square test.
Findings: The frequency of IL-10-592 C/C genotype was 16% in patients infected with HBV and 12% in healthy individuals and was not significantly different (P = 0.5). Also, the frequency of C variant allele was 55.5% in HBV infected patients and 53% in healthy individuals that was not statistically different (P = 0.6).
Conclusion: Based on our findings, there was not any relationship between IL-10-592 (A/C) polymorphism and susceptibility to HBV infection in our study population. |
| first_indexed | 2024-03-12T19:18:17Z |
| format | Article |
| id | doaj.art-c20e02093ab14a0fbcb2af60915ca84f |
| institution | Directory Open Access Journal |
| issn | 1027-7595 1735-854X |
| language | fas |
| last_indexed | 2024-03-12T19:18:17Z |
| publishDate | 2014-03-01 |
| publisher | Isfahan University of Medical Sciences |
| record_format | Article |
| series | مجله دانشکده پزشکی اصفهان |
| spelling | doaj.art-c20e02093ab14a0fbcb2af60915ca84f2023-08-02T05:22:40ZfasIsfahan University of Medical Sciencesمجله دانشکده پزشکی اصفهان1027-75951735-854X2014-03-0131271243424411524The Survey on -592 Polymorphism of Interlukin-10 in Hepatitis B Virus Infected PatientsMohammad Hassan Bagheri-Mansoori0Zohreh Sharifi1Mohammad Hossein Sanati2Abolhassan Shahzadeh-Fazeli3Mansoureh Farhangnia4Department of Cell and Molecular Biology, School of Basic Sciences and Technologies, University of Science and Culture, Tehran, IranAssociate Professor, Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, IranAssociate Professor, Department of Medical Genetics, National Institute for Genetic Engineering and Biotechnology, Tehran, IranAssociate Professor, Department of Genetics, Royan Institute for Reproductive Biomedicine, Academic Center for Education, Culture and Research (ACECR), Tehran, IranHuman and Animal Cell Bank, Iranian Biological Resource Center (IBRC), Academic Center for Education, Culture and Research (ACECR), Tehran, IranBackground: Infection with hepatitis B virus (HBV) may result in a number of different clinical outcomes. There are strong evidences in HBV infection that host genetic factors play a major role in determining the outcome of infection. Polymorphisms in the promoter region of interleukin 10 (IL-10) affect its production and confer susceptibility to inflammatory diseases. The aim of present study was to investigate the association between HBV infection and -592 polymorphism in the promoter region of the IL-10 gene in Iranian population. Methods: 100 HBV infected patients and 100 healthy individuals were randomly selected. Genomic DNA was extracted from blood buffy coat using the salting-out method. The IL-10-592(A/C) genotypes were determined using allele-specific polymerase chain reaction (PCR) method. The PCR products were electrophoresed on 1.5% agarose gel. The data were analyzed using chi-square test. Findings: The frequency of IL-10-592 C/C genotype was 16% in patients infected with HBV and 12% in healthy individuals and was not significantly different (P = 0.5). Also, the frequency of C variant allele was 55.5% in HBV infected patients and 53% in healthy individuals that was not statistically different (P = 0.6). Conclusion: Based on our findings, there was not any relationship between IL-10-592 (A/C) polymorphism and susceptibility to HBV infection in our study population.http://jims.mui.ac.ir/index.php/jims/article/view/3230Single nucleotide polymorphisms (SNPs)Interleukin-10 (IL-10)Hepatitis B virus (HBV)Allele-specific polymerase chain reaction |
| spellingShingle | Mohammad Hassan Bagheri-Mansoori Zohreh Sharifi Mohammad Hossein Sanati Abolhassan Shahzadeh-Fazeli Mansoureh Farhangnia The Survey on -592 Polymorphism of Interlukin-10 in Hepatitis B Virus Infected Patients مجله دانشکده پزشکی اصفهان Single nucleotide polymorphisms (SNPs) Interleukin-10 (IL-10) Hepatitis B virus (HBV) Allele-specific polymerase chain reaction |
| title | The Survey on -592 Polymorphism of Interlukin-10 in Hepatitis B Virus Infected Patients |
| title_full | The Survey on -592 Polymorphism of Interlukin-10 in Hepatitis B Virus Infected Patients |
| title_fullStr | The Survey on -592 Polymorphism of Interlukin-10 in Hepatitis B Virus Infected Patients |
| title_full_unstemmed | The Survey on -592 Polymorphism of Interlukin-10 in Hepatitis B Virus Infected Patients |
| title_short | The Survey on -592 Polymorphism of Interlukin-10 in Hepatitis B Virus Infected Patients |
| title_sort | survey on 592 polymorphism of interlukin 10 in hepatitis b virus infected patients |
| topic | Single nucleotide polymorphisms (SNPs) Interleukin-10 (IL-10) Hepatitis B virus (HBV) Allele-specific polymerase chain reaction |
| url | http://jims.mui.ac.ir/index.php/jims/article/view/3230 |
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