Heparin-binding proteins of seminal plasma in Nellore bulls Proteínas ligadoras à heparina do plasma seminal em touros Nelore
The aim of this study was to identify heparin-binding proteins (HBPs) in seminal plasma of Nellore (Bos taurus indicus) bulls. Bulls (n=4), 30-36 months old, 500-550kg with satisfactory seminal quality were selected. After the centrifugation, samples of the seminal plasma were pooled and the HBPs we...
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| Format: | Article |
| Language: | English |
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Universidade Federal de Santa Maria
2009-02-01
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| Series: | Ciência Rural |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782009000100048 |
| _version_ | 1811301469491560448 |
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| author | Carlos Eurico Fernandes Fabiana Ferreira de Souza Jayme Augusto Souza-Neto Paulo Eduardo Martins Ribola |
| author_facet | Carlos Eurico Fernandes Fabiana Ferreira de Souza Jayme Augusto Souza-Neto Paulo Eduardo Martins Ribola |
| author_sort | Carlos Eurico Fernandes |
| collection | DOAJ |
| description | The aim of this study was to identify heparin-binding proteins (HBPs) in seminal plasma of Nellore (Bos taurus indicus) bulls. Bulls (n=4), 30-36 months old, 500-550kg with satisfactory seminal quality were selected. After the centrifugation, samples of the seminal plasma were pooled and the HBPs were isolated by heparin-affinity chromatography. The recovered HBPs fractions were pooled. One-dimensional sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDSPAGE) 12.5% was performed in vertical minigels. Eight bands with molecular weights ranging from 15 to 63kDa were observed. Two proteins were identified (22 and 25kDa), similar to those previously described in Bos taurus taurus bulls. Other bands identified in this study (39, 53, 58 and 63kDa) have not been previously observed and possibly they are specific to Nellore semen.<br>O objetivo deste estudo foi identificar proteínas ligadoras à heparina no plasma seminal de touros Nelore (Bos taurus indicus). Para tanto, foram selecionados quatro touros entre 30 e 36 meses de idade e peso aproximado de 500-550kg. Após centrifugação, amostras do plasma seminal foram misturadas e as proteínas ligadoras à heparina foram isoladas por meio da cromatografia por afinidade. As frações após a eluição foram agrupadas para caracterização das bandas protéicas (SDSPAGE, 12,5%). Foram identificadas oito bandas protéicas variando entre 15 e 63kDa. Duas proteínas com 22 e 25kDa foram similares às descritas em touros Bos taurus taurus. Outras proteínas identificadas com 39, 53, 58 e 63kDa ainda não foram descritas e possivelmente sejam específicas para Bos taurus indicus. |
| first_indexed | 2024-04-13T07:09:22Z |
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| id | doaj.art-c2100d502c65472c9e910e258218608d |
| institution | Directory Open Access Journal |
| issn | 0103-8478 1678-4596 |
| language | English |
| last_indexed | 2024-04-13T07:09:22Z |
| publishDate | 2009-02-01 |
| publisher | Universidade Federal de Santa Maria |
| record_format | Article |
| series | Ciência Rural |
| spelling | doaj.art-c2100d502c65472c9e910e258218608d2022-12-22T02:56:55ZengUniversidade Federal de Santa MariaCiência Rural0103-84781678-45962009-02-01391275278Heparin-binding proteins of seminal plasma in Nellore bulls Proteínas ligadoras à heparina do plasma seminal em touros NeloreCarlos Eurico FernandesFabiana Ferreira de SouzaJayme Augusto Souza-NetoPaulo Eduardo Martins RibolaThe aim of this study was to identify heparin-binding proteins (HBPs) in seminal plasma of Nellore (Bos taurus indicus) bulls. Bulls (n=4), 30-36 months old, 500-550kg with satisfactory seminal quality were selected. After the centrifugation, samples of the seminal plasma were pooled and the HBPs were isolated by heparin-affinity chromatography. The recovered HBPs fractions were pooled. One-dimensional sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDSPAGE) 12.5% was performed in vertical minigels. Eight bands with molecular weights ranging from 15 to 63kDa were observed. Two proteins were identified (22 and 25kDa), similar to those previously described in Bos taurus taurus bulls. Other bands identified in this study (39, 53, 58 and 63kDa) have not been previously observed and possibly they are specific to Nellore semen.<br>O objetivo deste estudo foi identificar proteínas ligadoras à heparina no plasma seminal de touros Nelore (Bos taurus indicus). Para tanto, foram selecionados quatro touros entre 30 e 36 meses de idade e peso aproximado de 500-550kg. Após centrifugação, amostras do plasma seminal foram misturadas e as proteínas ligadoras à heparina foram isoladas por meio da cromatografia por afinidade. As frações após a eluição foram agrupadas para caracterização das bandas protéicas (SDSPAGE, 12,5%). Foram identificadas oito bandas protéicas variando entre 15 e 63kDa. Duas proteínas com 22 e 25kDa foram similares às descritas em touros Bos taurus taurus. Outras proteínas identificadas com 39, 53, 58 e 63kDa ainda não foram descritas e possivelmente sejam específicas para Bos taurus indicus.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782009000100048bovinosproteínasplasma seminalBos taurus indicusbovineproteinsseminal plasmaBos taurus indicus |
| spellingShingle | Carlos Eurico Fernandes Fabiana Ferreira de Souza Jayme Augusto Souza-Neto Paulo Eduardo Martins Ribola Heparin-binding proteins of seminal plasma in Nellore bulls Proteínas ligadoras à heparina do plasma seminal em touros Nelore Ciência Rural bovinos proteínas plasma seminal Bos taurus indicus bovine proteins seminal plasma Bos taurus indicus |
| title | Heparin-binding proteins of seminal plasma in Nellore bulls Proteínas ligadoras à heparina do plasma seminal em touros Nelore |
| title_full | Heparin-binding proteins of seminal plasma in Nellore bulls Proteínas ligadoras à heparina do plasma seminal em touros Nelore |
| title_fullStr | Heparin-binding proteins of seminal plasma in Nellore bulls Proteínas ligadoras à heparina do plasma seminal em touros Nelore |
| title_full_unstemmed | Heparin-binding proteins of seminal plasma in Nellore bulls Proteínas ligadoras à heparina do plasma seminal em touros Nelore |
| title_short | Heparin-binding proteins of seminal plasma in Nellore bulls Proteínas ligadoras à heparina do plasma seminal em touros Nelore |
| title_sort | heparin binding proteins of seminal plasma in nellore bulls proteinas ligadoras a heparina do plasma seminal em touros nelore |
| topic | bovinos proteínas plasma seminal Bos taurus indicus bovine proteins seminal plasma Bos taurus indicus |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782009000100048 |
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