Encapsulation of Fennel and Basil Essential Oils in β-Cyclodextrin for Novel Biopesticide Formulation
β-cyclodextrin (β-CD) is a good host for the encapsulation of fennel and basil essential oils (FEO and BEO, respectively) and the formation of inclusion complexes (ICs) using the co-precipitation method. According to the results of the GC/MS analysis conducted in this study, monoterpenes and monoter...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-03-01
|
| Series: | Biomolecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2218-273X/14/3/353 |
| _version_ | 1827306915158294528 |
|---|---|
| author | Nina Devrnja Boban Anđelković Jovana Ljujić Tatjana Ćosić Sofija Stupar Milica Milutinović Jelena Savić |
| author_facet | Nina Devrnja Boban Anđelković Jovana Ljujić Tatjana Ćosić Sofija Stupar Milica Milutinović Jelena Savić |
| author_sort | Nina Devrnja |
| collection | DOAJ |
| description | β-cyclodextrin (β-CD) is a good host for the encapsulation of fennel and basil essential oils (FEO and BEO, respectively) and the formation of inclusion complexes (ICs) using the co-precipitation method. According to the results of the GC/MS analysis conducted in this study, monoterpenes and monoterpenoids were the dominant chemical groups in total FEO, while in BEO, these two groups occurred along with sesquiterpenes and sesquiterpenoids. The presence of dominant compounds from both EOs was validated using the FT-IR spectra of ICs, which indicated successful complexation. Analyses conducted using SPME/GC-MS showed the continuous emission of volatiles over 24 h from both ICs. Under SEM, particles of both ICs appeared to have a rectangular or rhomboid morphology and few aggregates. The insecticidal properties of EOs and ICs with β-CD were tested on the Colorado potato beetle (CPB) as a model pest. The inclusion complex of β-CD with FEO altered the developmental dynamic and body mass of the CPB. The initial increase in the proteolytic activity of CPB larvae fed with potato plants sprayed with ICs was not maintained for long, and the proteolytic efficacy of treated larvae remained in line with that of the control larvae. Future investigations will focus on manipulating the volume of EOs used and the treatment duration for optimal efficacy and potential application. |
| first_indexed | 2024-04-24T18:30:30Z |
| format | Article |
| id | doaj.art-c2106d65caf84af6bded9326e63b0051 |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-04-24T18:30:30Z |
| publishDate | 2024-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-c2106d65caf84af6bded9326e63b00512024-03-27T13:28:01ZengMDPI AGBiomolecules2218-273X2024-03-0114335310.3390/biom14030353Encapsulation of Fennel and Basil Essential Oils in β-Cyclodextrin for Novel Biopesticide FormulationNina Devrnja0Boban Anđelković1Jovana Ljujić2Tatjana Ćosić3Sofija Stupar4Milica Milutinović5Jelena Savić6Institute for Biological Research “Siniša Stanković”—National Institute of the Republic of Serbia, University of Belgrade, Bulevar despota Stefana 142, 11108 Belgrade, SerbiaFaculty of Chemistry, University of Belgrade, Studentski trg 16, 11000 Belgrade, SerbiaFaculty of Chemistry, University of Belgrade, Studentski trg 16, 11000 Belgrade, SerbiaInstitute for Biological Research “Siniša Stanković”—National Institute of the Republic of Serbia, University of Belgrade, Bulevar despota Stefana 142, 11108 Belgrade, SerbiaInstitute for Biological Research “Siniša Stanković”—National Institute of the Republic of Serbia, University of Belgrade, Bulevar despota Stefana 142, 11108 Belgrade, SerbiaInstitute for Biological Research “Siniša Stanković”—National Institute of the Republic of Serbia, University of Belgrade, Bulevar despota Stefana 142, 11108 Belgrade, SerbiaInstitute for Biological Research “Siniša Stanković”—National Institute of the Republic of Serbia, University of Belgrade, Bulevar despota Stefana 142, 11108 Belgrade, Serbiaβ-cyclodextrin (β-CD) is a good host for the encapsulation of fennel and basil essential oils (FEO and BEO, respectively) and the formation of inclusion complexes (ICs) using the co-precipitation method. According to the results of the GC/MS analysis conducted in this study, monoterpenes and monoterpenoids were the dominant chemical groups in total FEO, while in BEO, these two groups occurred along with sesquiterpenes and sesquiterpenoids. The presence of dominant compounds from both EOs was validated using the FT-IR spectra of ICs, which indicated successful complexation. Analyses conducted using SPME/GC-MS showed the continuous emission of volatiles over 24 h from both ICs. Under SEM, particles of both ICs appeared to have a rectangular or rhomboid morphology and few aggregates. The insecticidal properties of EOs and ICs with β-CD were tested on the Colorado potato beetle (CPB) as a model pest. The inclusion complex of β-CD with FEO altered the developmental dynamic and body mass of the CPB. The initial increase in the proteolytic activity of CPB larvae fed with potato plants sprayed with ICs was not maintained for long, and the proteolytic efficacy of treated larvae remained in line with that of the control larvae. Future investigations will focus on manipulating the volume of EOs used and the treatment duration for optimal efficacy and potential application.https://www.mdpi.com/2218-273X/14/3/353inclusion complexFT-IRSPME/GC-MSColorado potato beetle |
| spellingShingle | Nina Devrnja Boban Anđelković Jovana Ljujić Tatjana Ćosić Sofija Stupar Milica Milutinović Jelena Savić Encapsulation of Fennel and Basil Essential Oils in β-Cyclodextrin for Novel Biopesticide Formulation Biomolecules inclusion complex FT-IR SPME/GC-MS Colorado potato beetle |
| title | Encapsulation of Fennel and Basil Essential Oils in β-Cyclodextrin for Novel Biopesticide Formulation |
| title_full | Encapsulation of Fennel and Basil Essential Oils in β-Cyclodextrin for Novel Biopesticide Formulation |
| title_fullStr | Encapsulation of Fennel and Basil Essential Oils in β-Cyclodextrin for Novel Biopesticide Formulation |
| title_full_unstemmed | Encapsulation of Fennel and Basil Essential Oils in β-Cyclodextrin for Novel Biopesticide Formulation |
| title_short | Encapsulation of Fennel and Basil Essential Oils in β-Cyclodextrin for Novel Biopesticide Formulation |
| title_sort | encapsulation of fennel and basil essential oils in β cyclodextrin for novel biopesticide formulation |
| topic | inclusion complex FT-IR SPME/GC-MS Colorado potato beetle |
| url | https://www.mdpi.com/2218-273X/14/3/353 |
| work_keys_str_mv | AT ninadevrnja encapsulationoffennelandbasilessentialoilsinbcyclodextrinfornovelbiopesticideformulation AT bobananđelkovic encapsulationoffennelandbasilessentialoilsinbcyclodextrinfornovelbiopesticideformulation AT jovanaljujic encapsulationoffennelandbasilessentialoilsinbcyclodextrinfornovelbiopesticideformulation AT tatjanacosic encapsulationoffennelandbasilessentialoilsinbcyclodextrinfornovelbiopesticideformulation AT sofijastupar encapsulationoffennelandbasilessentialoilsinbcyclodextrinfornovelbiopesticideformulation AT milicamilutinovic encapsulationoffennelandbasilessentialoilsinbcyclodextrinfornovelbiopesticideformulation AT jelenasavic encapsulationoffennelandbasilessentialoilsinbcyclodextrinfornovelbiopesticideformulation |