Extended spectrum β-lactamase producing uropathogenic Escherichia coli and the correlation of biofilm with antibiotics resistance in Nepal
Abstract Background Urinary tract infection (UTI) is one of the frequently diagnosed infectious diseases which is caused mainly by Escherichia coli. E. coli confers resistance against the two major classes of antibiotics due to the production of extended spectrum β-lactamase enzymes (ESBL), biofilm,...
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| Format: | Article |
| Language: | English |
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BMC
2019-12-01
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| Series: | Annals of Clinical Microbiology and Antimicrobials |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12941-019-0340-y |
| _version_ | 1818569959906738176 |
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| author | Raju Shrestha Santosh Khanal Pramod Poudel Karan Khadayat Sajani Ghaju Anita Bhandari Sunil Lekhak Narayan Dutt Pant Manisha Sharma Bishnu P. Marasini |
| author_facet | Raju Shrestha Santosh Khanal Pramod Poudel Karan Khadayat Sajani Ghaju Anita Bhandari Sunil Lekhak Narayan Dutt Pant Manisha Sharma Bishnu P. Marasini |
| author_sort | Raju Shrestha |
| collection | DOAJ |
| description | Abstract Background Urinary tract infection (UTI) is one of the frequently diagnosed infectious diseases which is caused mainly by Escherichia coli. E. coli confers resistance against the two major classes of antibiotics due to the production of extended spectrum β-lactamase enzymes (ESBL), biofilm, etc. Biofilm produced by uropathogenic E. coli (UPEC) protects from host immune system and prevent entry of antimicrobial compounds. The main objective of this cross-sectional study was to determine the correlation of biofilm production and antibiotic resistance as well as to characterize the pgaA and pgaC genes responsible for biofilm formation among uropathogenic ESBL producing E. coli. Methods A total of 1977 mid-stream urine samples were examined and cultured for bacterial strain identification. ESBL was detected by combined disc method following CLSI whereas biofilm formation was analyzed by semi-quantitative method. Furthermore, the pgaA and pgaC genes responsible for biofilm formation in UPEC were detected by multiplex PCR. All the statistical analyses were done via IBM SPSS Statistics 21 where Pearson’s correlation test were used to determine correlation (−1 ≥ r ≤ 1). Results E. coli was the predominant causative agent, which accounted 159 (59.3%) of the Gram-negative bacteria, where 81 (50.9%) E. coli strains were found to be ESBL producers. In addition, 86 (54.1%) E. coli strains were found to be biofilm producers. Both the pgaA and pgaC genes were detected in 45 (93.7%) the UPEC isolates, which were both biofilm and ESBL producers. Moreover, there was a positive correlation between biofilm and ESBL production. Conclusion The analyses presented weak positive correlation between biofilm and ESBL production in which biofilm producing UPEC harbors both pgaA and pgaC genes responsible for biofilm formation. |
| first_indexed | 2024-12-14T06:54:31Z |
| format | Article |
| id | doaj.art-c2122db8ff3245b8875f65013f4326c6 |
| institution | Directory Open Access Journal |
| issn | 1476-0711 |
| language | English |
| last_indexed | 2024-12-14T06:54:31Z |
| publishDate | 2019-12-01 |
| publisher | BMC |
| record_format | Article |
| series | Annals of Clinical Microbiology and Antimicrobials |
| spelling | doaj.art-c2122db8ff3245b8875f65013f4326c62022-12-21T23:12:45ZengBMCAnnals of Clinical Microbiology and Antimicrobials1476-07112019-12-011811610.1186/s12941-019-0340-yExtended spectrum β-lactamase producing uropathogenic Escherichia coli and the correlation of biofilm with antibiotics resistance in NepalRaju Shrestha0Santosh Khanal1Pramod Poudel2Karan Khadayat3Sajani Ghaju4Anita Bhandari5Sunil Lekhak6Narayan Dutt Pant7Manisha Sharma8Bishnu P. Marasini9Department of Microbiology, National College, Tribhuvan UniversityDepartment of Microbiology, National College, Tribhuvan UniversityDepartment of Microbiology, National College, Tribhuvan UniversityDepartment of Biotechnology, National College, Tribhuvan UniversityDepartment of Biotechnology, National College, Tribhuvan UniversityDepartment of Microbiology, Goldengate International College, Tribhuvan UniversityDepartment of Microbiology, National College, Tribhuvan UniversityDepartment of Microbiology, Grande International HospitalDepartment of Microbiology, Grande International HospitalDepartment of Biotechnology, National College, Tribhuvan UniversityAbstract Background Urinary tract infection (UTI) is one of the frequently diagnosed infectious diseases which is caused mainly by Escherichia coli. E. coli confers resistance against the two major classes of antibiotics due to the production of extended spectrum β-lactamase enzymes (ESBL), biofilm, etc. Biofilm produced by uropathogenic E. coli (UPEC) protects from host immune system and prevent entry of antimicrobial compounds. The main objective of this cross-sectional study was to determine the correlation of biofilm production and antibiotic resistance as well as to characterize the pgaA and pgaC genes responsible for biofilm formation among uropathogenic ESBL producing E. coli. Methods A total of 1977 mid-stream urine samples were examined and cultured for bacterial strain identification. ESBL was detected by combined disc method following CLSI whereas biofilm formation was analyzed by semi-quantitative method. Furthermore, the pgaA and pgaC genes responsible for biofilm formation in UPEC were detected by multiplex PCR. All the statistical analyses were done via IBM SPSS Statistics 21 where Pearson’s correlation test were used to determine correlation (−1 ≥ r ≤ 1). Results E. coli was the predominant causative agent, which accounted 159 (59.3%) of the Gram-negative bacteria, where 81 (50.9%) E. coli strains were found to be ESBL producers. In addition, 86 (54.1%) E. coli strains were found to be biofilm producers. Both the pgaA and pgaC genes were detected in 45 (93.7%) the UPEC isolates, which were both biofilm and ESBL producers. Moreover, there was a positive correlation between biofilm and ESBL production. Conclusion The analyses presented weak positive correlation between biofilm and ESBL production in which biofilm producing UPEC harbors both pgaA and pgaC genes responsible for biofilm formation.https://doi.org/10.1186/s12941-019-0340-yE. coliUTIESBLBiofilmpgaA and pgaC |
| spellingShingle | Raju Shrestha Santosh Khanal Pramod Poudel Karan Khadayat Sajani Ghaju Anita Bhandari Sunil Lekhak Narayan Dutt Pant Manisha Sharma Bishnu P. Marasini Extended spectrum β-lactamase producing uropathogenic Escherichia coli and the correlation of biofilm with antibiotics resistance in Nepal Annals of Clinical Microbiology and Antimicrobials E. coli UTI ESBL Biofilm pgaA and pgaC |
| title | Extended spectrum β-lactamase producing uropathogenic Escherichia coli and the correlation of biofilm with antibiotics resistance in Nepal |
| title_full | Extended spectrum β-lactamase producing uropathogenic Escherichia coli and the correlation of biofilm with antibiotics resistance in Nepal |
| title_fullStr | Extended spectrum β-lactamase producing uropathogenic Escherichia coli and the correlation of biofilm with antibiotics resistance in Nepal |
| title_full_unstemmed | Extended spectrum β-lactamase producing uropathogenic Escherichia coli and the correlation of biofilm with antibiotics resistance in Nepal |
| title_short | Extended spectrum β-lactamase producing uropathogenic Escherichia coli and the correlation of biofilm with antibiotics resistance in Nepal |
| title_sort | extended spectrum β lactamase producing uropathogenic escherichia coli and the correlation of biofilm with antibiotics resistance in nepal |
| topic | E. coli UTI ESBL Biofilm pgaA and pgaC |
| url | https://doi.org/10.1186/s12941-019-0340-y |
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