A Systematic Detection for Brucellosis at Chronic Stage of Infection in Semen of Sheep and Saanen Goats
The study was conducted in a herd (n: 244) in which goats (n: 206) and sheep (n:38) had a history of brucellosis in Bursa which is located in Northwestern of Turkey between the years 2012-2014. For the detection of Brucella spp. and the other zoonotic bacterial agents, semen samples were taken from...
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| Format: | Article |
| Language: | English |
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Turkish Science and Technology Publishing (TURSTEP)
2019-10-01
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| Series: | Turkish Journal of Agriculture: Food Science and Technology |
| Subjects: | |
| Online Access: | http://www.agrifoodscience.com/index.php/TURJAF/article/view/2805 |
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| author | Esra Buyukcangaz Burcu Ustuner Sevil Erdenlig Selim Alcay Huban Gocmen Berk Toker Engin Kennerman Mihriban Ulgen |
| author_facet | Esra Buyukcangaz Burcu Ustuner Sevil Erdenlig Selim Alcay Huban Gocmen Berk Toker Engin Kennerman Mihriban Ulgen |
| author_sort | Esra Buyukcangaz |
| collection | DOAJ |
| description | The study was conducted in a herd (n: 244) in which goats (n: 206) and sheep (n:38) had a history of brucellosis in Bursa which is located in Northwestern of Turkey between the years 2012-2014. For the detection of Brucella spp. and the other zoonotic bacterial agents, semen samples were taken from Saanen goats (n: 35) and rams (n: 8). Samples were tested by routine diagnostic procedures and PCR. The serum samples of male animals were also tested for Brucellosis by C-ELISA and I-ELISA. The culture results represented Trueperella pyogenes (n:2), Pasteurella pneumotopica (n: 5), Esherichia coli (n: 3), Aeromonas salmonicida subs. Salmonicida (1), Brevundimonas vesicularis (n: 2) and Mycoplasma bovigenitalium (n: 1) and Mycoplasma arginini (n: 1) from semen samples. Rams had no symptoms due to epididymitis or epididymoorchitis in clinical examination, but two bucks showed orchitis and they were serologically positive for brucellosis. Also, one seronegative buck showed epididymitis in a flock. There were no statistically significant differences between the serologically positive and negative animals in an examination of semen samples in terms of their volume, concentration, mass activity, motility and defectivity rate for acrosome. Although 20 of the serum samples were negative for anti-Brucella antibody, 23 of them were serologically positive for brucellosis. As a result of this study, Brucellae were not detected by bacteriologically and molecularly while there were some positive serum samples for brucellosis. This could be attributed that these samples might have been collected from chronically infected animals in which animals generally do not shed the organisms. Therefore, it was thought that sampling with regular intervals might help for the definitive incidence of brucellosis. |
| first_indexed | 2024-04-10T14:40:47Z |
| format | Article |
| id | doaj.art-c213156a02784d16b3f8dc1d5fbf7034 |
| institution | Directory Open Access Journal |
| issn | 2148-127X |
| language | English |
| last_indexed | 2024-04-10T14:40:47Z |
| publishDate | 2019-10-01 |
| publisher | Turkish Science and Technology Publishing (TURSTEP) |
| record_format | Article |
| series | Turkish Journal of Agriculture: Food Science and Technology |
| spelling | doaj.art-c213156a02784d16b3f8dc1d5fbf70342023-02-15T16:08:14ZengTurkish Science and Technology Publishing (TURSTEP)Turkish Journal of Agriculture: Food Science and Technology2148-127X2019-10-017101670167710.24925/turjaf.v7i10.1670-1677.28051284A Systematic Detection for Brucellosis at Chronic Stage of Infection in Semen of Sheep and Saanen GoatsEsra Buyukcangaz0Burcu Ustuner1Sevil Erdenlig2Selim Alcay3Huban Gocmen4Berk Toker5Engin Kennerman6Mihriban Ulgen7Department of Microbiology, Faculty of Veterinary Medicine, Bursa Uludağ University, 16059 Nilufer/BursaDepartment of Artificial Insemination, Faculty of Veterinary Medicine, Bursa Uludağ University, 16059 Nilufer/BursaDepartment of Microbiology, Faculty of Veterinary Medicine, Harran University, 63200 Eyyübiye/Şanlıurfa,Department of Artificial Insemination, Faculty of Veterinary Medicine, Bursa Uludağ University, 16059 Nilufer/BursaDepartment of Microbiology, Faculty of Veterinary Medicine, Near East University, 99138 Nicosia/Northern CyprusDepartment of Artificial Insemination, Faculty of Veterinary Medicine, Bursa Uludağ University, 16059 Nilufer/BursaDepartment of Internal Medicine, Faculty of Veterinary, Medicine Bursa Uludag University,16059 Niılufer/BursaDepartment of Microbiology, Faculty of Veterinary Medicine, Bursa Uludağ University, 16059 Nilufer/BursaThe study was conducted in a herd (n: 244) in which goats (n: 206) and sheep (n:38) had a history of brucellosis in Bursa which is located in Northwestern of Turkey between the years 2012-2014. For the detection of Brucella spp. and the other zoonotic bacterial agents, semen samples were taken from Saanen goats (n: 35) and rams (n: 8). Samples were tested by routine diagnostic procedures and PCR. The serum samples of male animals were also tested for Brucellosis by C-ELISA and I-ELISA. The culture results represented Trueperella pyogenes (n:2), Pasteurella pneumotopica (n: 5), Esherichia coli (n: 3), Aeromonas salmonicida subs. Salmonicida (1), Brevundimonas vesicularis (n: 2) and Mycoplasma bovigenitalium (n: 1) and Mycoplasma arginini (n: 1) from semen samples. Rams had no symptoms due to epididymitis or epididymoorchitis in clinical examination, but two bucks showed orchitis and they were serologically positive for brucellosis. Also, one seronegative buck showed epididymitis in a flock. There were no statistically significant differences between the serologically positive and negative animals in an examination of semen samples in terms of their volume, concentration, mass activity, motility and defectivity rate for acrosome. Although 20 of the serum samples were negative for anti-Brucella antibody, 23 of them were serologically positive for brucellosis. As a result of this study, Brucellae were not detected by bacteriologically and molecularly while there were some positive serum samples for brucellosis. This could be attributed that these samples might have been collected from chronically infected animals in which animals generally do not shed the organisms. Therefore, it was thought that sampling with regular intervals might help for the definitive incidence of brucellosis.http://www.agrifoodscience.com/index.php/TURJAF/article/view/2805goatrambrucellosissemenelisapcr |
| spellingShingle | Esra Buyukcangaz Burcu Ustuner Sevil Erdenlig Selim Alcay Huban Gocmen Berk Toker Engin Kennerman Mihriban Ulgen A Systematic Detection for Brucellosis at Chronic Stage of Infection in Semen of Sheep and Saanen Goats Turkish Journal of Agriculture: Food Science and Technology goat ram brucellosis semen elisa pcr |
| title | A Systematic Detection for Brucellosis at Chronic Stage of Infection in Semen of Sheep and Saanen Goats |
| title_full | A Systematic Detection for Brucellosis at Chronic Stage of Infection in Semen of Sheep and Saanen Goats |
| title_fullStr | A Systematic Detection for Brucellosis at Chronic Stage of Infection in Semen of Sheep and Saanen Goats |
| title_full_unstemmed | A Systematic Detection for Brucellosis at Chronic Stage of Infection in Semen of Sheep and Saanen Goats |
| title_short | A Systematic Detection for Brucellosis at Chronic Stage of Infection in Semen of Sheep and Saanen Goats |
| title_sort | systematic detection for brucellosis at chronic stage of infection in semen of sheep and saanen goats |
| topic | goat ram brucellosis semen elisa pcr |
| url | http://www.agrifoodscience.com/index.php/TURJAF/article/view/2805 |
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