Diarrhoea in neonatal piglets: a case control study on microbiological findings
Abstract Background Many factors can influence the occurrence of neonatal diarrhoea in piglets. Currently, well-known pathogens such as enterotoxigenic Escherichia coli and Clostridium perfringens type C appear to play a minor role in development of disease. Other infectious pathogens may be involve...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2018-09-01
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| Series: | Porcine Health Management |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s40813-018-0094-5 |
| _version_ | 1818595592266317824 |
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| author | Hanne Kongsted Karl Pedersen Charlotte Kristiane Hjulsager Lars Erik Larsen Ken Steen Pedersen Sven Erik Jorsal Poul Bækbo |
| author_facet | Hanne Kongsted Karl Pedersen Charlotte Kristiane Hjulsager Lars Erik Larsen Ken Steen Pedersen Sven Erik Jorsal Poul Bækbo |
| author_sort | Hanne Kongsted |
| collection | DOAJ |
| description | Abstract Background Many factors can influence the occurrence of neonatal diarrhoea in piglets. Currently, well-known pathogens such as enterotoxigenic Escherichia coli and Clostridium perfringens type C appear to play a minor role in development of disease. Other infectious pathogens may be involved. In this study, we aimed to investigate the presence of selected infectious pathogens in neonatal piglets with clinical and pathological signs of enteric disease. The association between rotavirus A, Enterococcus hirae, Clostridium difficile and Clostridium perfringens type A/C and diarrhoea was investigated in a case control study on piglet level. The possible role of E. coli virulence factors was investigated in a multistep-procedure using herd-pools of E.coli isolates to screen for their presence. Results Rotavirus A was detected more often in cases (25%) than in controls (6%) (P < 0.001). The detection rate of Enterococcus hirae, Clostridium difficile and C. perfringens type A positive for beta2 genes was the same in the two groups of piglets. C. perfringens type C was not detected in the study. Investigations on E. coli virulence factors showed a high prevalence of EAST1 toxin genes (55% of tested case piglets were positive) and AIDA-1 adhesin genes (63% of toxin positive case piglets were positive) in case piglets. Conclusions Detection of rotavirus A was statistically significantly associated with neonatal piglet diarrhoea. An aetiologic role of E. coli carrying virulence factors EAST1 and AIDA-1 needs further investigation as the study points out these two factors as possible causative factors in neonatal diarrhoea. Detection of E.hirae, C.difficile and C. perfringens type A carrying beta 2 genes was not associated with neonatal piglet diarrhoea. However, the study suggested that massive overgrowth by E. hirae could be part of the pathogenesis in some cases of neonatal diarrhoea. |
| first_indexed | 2024-12-16T11:18:28Z |
| format | Article |
| id | doaj.art-c214411ea93d47e7b1f0cd8f9fca986f |
| institution | Directory Open Access Journal |
| issn | 2055-5660 |
| language | English |
| last_indexed | 2024-12-16T11:18:28Z |
| publishDate | 2018-09-01 |
| publisher | BMC |
| record_format | Article |
| series | Porcine Health Management |
| spelling | doaj.art-c214411ea93d47e7b1f0cd8f9fca986f2022-12-21T22:33:32ZengBMCPorcine Health Management2055-56602018-09-01411710.1186/s40813-018-0094-5Diarrhoea in neonatal piglets: a case control study on microbiological findingsHanne Kongsted0Karl Pedersen1Charlotte Kristiane Hjulsager2Lars Erik Larsen3Ken Steen Pedersen4Sven Erik Jorsal5Poul Bækbo6Department of Animal Science, Aarhus UniversityNational Veterinary Institute, Technical University of DenmarkNational Veterinary Institute, Technical University of DenmarkNational Veterinary Institute, Technical University of DenmarkSEGES Danish Pig Research CentreNational Veterinary Institute, Technical University of DenmarkSEGES Danish Pig Research CentreAbstract Background Many factors can influence the occurrence of neonatal diarrhoea in piglets. Currently, well-known pathogens such as enterotoxigenic Escherichia coli and Clostridium perfringens type C appear to play a minor role in development of disease. Other infectious pathogens may be involved. In this study, we aimed to investigate the presence of selected infectious pathogens in neonatal piglets with clinical and pathological signs of enteric disease. The association between rotavirus A, Enterococcus hirae, Clostridium difficile and Clostridium perfringens type A/C and diarrhoea was investigated in a case control study on piglet level. The possible role of E. coli virulence factors was investigated in a multistep-procedure using herd-pools of E.coli isolates to screen for their presence. Results Rotavirus A was detected more often in cases (25%) than in controls (6%) (P < 0.001). The detection rate of Enterococcus hirae, Clostridium difficile and C. perfringens type A positive for beta2 genes was the same in the two groups of piglets. C. perfringens type C was not detected in the study. Investigations on E. coli virulence factors showed a high prevalence of EAST1 toxin genes (55% of tested case piglets were positive) and AIDA-1 adhesin genes (63% of toxin positive case piglets were positive) in case piglets. Conclusions Detection of rotavirus A was statistically significantly associated with neonatal piglet diarrhoea. An aetiologic role of E. coli carrying virulence factors EAST1 and AIDA-1 needs further investigation as the study points out these two factors as possible causative factors in neonatal diarrhoea. Detection of E.hirae, C.difficile and C. perfringens type A carrying beta 2 genes was not associated with neonatal piglet diarrhoea. However, the study suggested that massive overgrowth by E. hirae could be part of the pathogenesis in some cases of neonatal diarrhoea.http://link.springer.com/article/10.1186/s40813-018-0094-5PigletsNeonatal diarrhoeaRotavirus aE. coli virulence factorsEAST1AIDA-1 |
| spellingShingle | Hanne Kongsted Karl Pedersen Charlotte Kristiane Hjulsager Lars Erik Larsen Ken Steen Pedersen Sven Erik Jorsal Poul Bækbo Diarrhoea in neonatal piglets: a case control study on microbiological findings Porcine Health Management Piglets Neonatal diarrhoea Rotavirus a E. coli virulence factors EAST1 AIDA-1 |
| title | Diarrhoea in neonatal piglets: a case control study on microbiological findings |
| title_full | Diarrhoea in neonatal piglets: a case control study on microbiological findings |
| title_fullStr | Diarrhoea in neonatal piglets: a case control study on microbiological findings |
| title_full_unstemmed | Diarrhoea in neonatal piglets: a case control study on microbiological findings |
| title_short | Diarrhoea in neonatal piglets: a case control study on microbiological findings |
| title_sort | diarrhoea in neonatal piglets a case control study on microbiological findings |
| topic | Piglets Neonatal diarrhoea Rotavirus a E. coli virulence factors EAST1 AIDA-1 |
| url | http://link.springer.com/article/10.1186/s40813-018-0094-5 |
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