Expression pattern and clinical significance of β-catenin gene and protein in patients with primary malignant and benign bone tumors
Abstract This study is aimed to unravel the status of local and circulating β-catenin in different primary bone tumors and its relevance to tumor types, severity, and chemotherapy. The β-catenin mRNA expression level and the expression of the protein (intensity level) were evaluated in tumor tissue...
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| Format: | Article |
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Nature Portfolio
2022-06-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-13685-1 |
| _version_ | 1828219952048373760 |
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| author | Narges Khademian Alireza Mirzaei Ameinh Hosseini Leila Zare Shima Nazem Pegah Babaheidarian Alireza Sheikhi Zohreh Abdolvahabi Mostafa Ibrahimi Khodamorad Jamshidi Mahtab Rahbar Vahid Salimi Masoumeh Tavakoli-Yaraki |
| author_facet | Narges Khademian Alireza Mirzaei Ameinh Hosseini Leila Zare Shima Nazem Pegah Babaheidarian Alireza Sheikhi Zohreh Abdolvahabi Mostafa Ibrahimi Khodamorad Jamshidi Mahtab Rahbar Vahid Salimi Masoumeh Tavakoli-Yaraki |
| author_sort | Narges Khademian |
| collection | DOAJ |
| description | Abstract This study is aimed to unravel the status of local and circulating β-catenin in different primary bone tumors and its relevance to tumor types, severity, and chemotherapy. The β-catenin mRNA expression level and the expression of the protein (intensity level) were evaluated in tumor tissue and peripheral blood mononuclear cells of 150 patients with different types of primary bone tumors (78 malignant and 72 benign tumors) using Real-Time PCR and immunohistochemistry. The β-catenin mRNA expression level and the expression of the protein were increased in bone tumors which was positively correlated with the tumor malignancy. Amongst osteosarcoma, Ewing's Sarcoma, chondrosarcoma, osteochondroma, Giant Cell Tumor, and exostosis tumors, the osteosarcoma, and Giant Cell Tumor groups showed the highest level of β-catenin expression. The β-catenin expression in malignant bone tumors was significantly correlated with tumor grade, size, metastasis, tumor recurrent, and the level of response to chemotherapy. A similar pattern of β-catenin gene expression and its association with tumor characteristics was detected in the patient's peripheral blood cells. The simultaneous increase in the expression of the β-catenin gene and protein in tumor tissue and in circulating blood cells and its relationship with tumor severity indicates the possible promoting role of β-catenin in primary bone tumor pathogenesis. |
| first_indexed | 2024-04-12T16:20:18Z |
| format | Article |
| id | doaj.art-c21a769a463c41f18b95ecebcc913f9c |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-12T16:20:18Z |
| publishDate | 2022-06-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-c21a769a463c41f18b95ecebcc913f9c2022-12-22T03:25:34ZengNature PortfolioScientific Reports2045-23222022-06-0112111510.1038/s41598-022-13685-1Expression pattern and clinical significance of β-catenin gene and protein in patients with primary malignant and benign bone tumorsNarges Khademian0Alireza Mirzaei1Ameinh Hosseini2Leila Zare3Shima Nazem4Pegah Babaheidarian5Alireza Sheikhi6Zohreh Abdolvahabi7Mostafa Ibrahimi8Khodamorad Jamshidi9Mahtab Rahbar10Vahid Salimi11Masoumeh Tavakoli-Yaraki12Department of Biochemistry, School of Medicine, Iran University of Medical SciencesBone and Joint Reconstruction Research Center, Shafa Orthopedic Hospital, Iran University of Medical SciencesDepartment of Biochemistry, School of Medicine, Iran University of Medical SciencesDepartment of Physiology, Faculty of Medical Sciences, Tarbiat Modares UniversityDepartment of Laboratory Medicine, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical SciencesDepartment of Pathology, School of Medicine, Iran University of Medical SciencesDepartment of Biochemistry, School of Medicine, Iran University of Medical SciencesMetabolic Diseases Research Center, Research Institute for Prevention of Non-Communicable Disease, Cellular and Molecular Research Center, Qazvin University of Medical SciencesDepartment of Clinical Biochemistry, School of Medicine, Tarbiat Modares UniversityBone and Joint Reconstruction Research Center, Shafa Orthopedic Hospital, Iran University of Medical SciencesDepartment of Pathology, School of Medicine, Iran University of Medical SciencesDepartment of Virology, School of Public Health, Tehran University of Medical SciencesDepartment of Biochemistry, School of Medicine, Iran University of Medical SciencesAbstract This study is aimed to unravel the status of local and circulating β-catenin in different primary bone tumors and its relevance to tumor types, severity, and chemotherapy. The β-catenin mRNA expression level and the expression of the protein (intensity level) were evaluated in tumor tissue and peripheral blood mononuclear cells of 150 patients with different types of primary bone tumors (78 malignant and 72 benign tumors) using Real-Time PCR and immunohistochemistry. The β-catenin mRNA expression level and the expression of the protein were increased in bone tumors which was positively correlated with the tumor malignancy. Amongst osteosarcoma, Ewing's Sarcoma, chondrosarcoma, osteochondroma, Giant Cell Tumor, and exostosis tumors, the osteosarcoma, and Giant Cell Tumor groups showed the highest level of β-catenin expression. The β-catenin expression in malignant bone tumors was significantly correlated with tumor grade, size, metastasis, tumor recurrent, and the level of response to chemotherapy. A similar pattern of β-catenin gene expression and its association with tumor characteristics was detected in the patient's peripheral blood cells. The simultaneous increase in the expression of the β-catenin gene and protein in tumor tissue and in circulating blood cells and its relationship with tumor severity indicates the possible promoting role of β-catenin in primary bone tumor pathogenesis.https://doi.org/10.1038/s41598-022-13685-1 |
| spellingShingle | Narges Khademian Alireza Mirzaei Ameinh Hosseini Leila Zare Shima Nazem Pegah Babaheidarian Alireza Sheikhi Zohreh Abdolvahabi Mostafa Ibrahimi Khodamorad Jamshidi Mahtab Rahbar Vahid Salimi Masoumeh Tavakoli-Yaraki Expression pattern and clinical significance of β-catenin gene and protein in patients with primary malignant and benign bone tumors Scientific Reports |
| title | Expression pattern and clinical significance of β-catenin gene and protein in patients with primary malignant and benign bone tumors |
| title_full | Expression pattern and clinical significance of β-catenin gene and protein in patients with primary malignant and benign bone tumors |
| title_fullStr | Expression pattern and clinical significance of β-catenin gene and protein in patients with primary malignant and benign bone tumors |
| title_full_unstemmed | Expression pattern and clinical significance of β-catenin gene and protein in patients with primary malignant and benign bone tumors |
| title_short | Expression pattern and clinical significance of β-catenin gene and protein in patients with primary malignant and benign bone tumors |
| title_sort | expression pattern and clinical significance of β catenin gene and protein in patients with primary malignant and benign bone tumors |
| url | https://doi.org/10.1038/s41598-022-13685-1 |
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