Honey bee hemocyte profiling by flow cytometry.
Multiple stress factors in honey bees are causing loss of bee colonies worldwide. Several infectious agents of bees are believed to contribute to this problem. The mechanisms of honey bee immunity are not completely understood, in part due to limited information about the types and abundances of hem...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2014-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4186811?pdf=render |
| _version_ | 1819083937913241600 |
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| author | William J Marringa Michael J Krueger Nancy L Burritt James B Burritt |
| author_facet | William J Marringa Michael J Krueger Nancy L Burritt James B Burritt |
| author_sort | William J Marringa |
| collection | DOAJ |
| description | Multiple stress factors in honey bees are causing loss of bee colonies worldwide. Several infectious agents of bees are believed to contribute to this problem. The mechanisms of honey bee immunity are not completely understood, in part due to limited information about the types and abundances of hemocytes that help bees resist disease. Our study utilized flow cytometry and microscopy to examine populations of hemolymph particulates in honey bees. We found bee hemolymph includes permeabilized cells, plasmatocytes, and acellular objects that resemble microparticles, listed in order of increasing abundance. The permeabilized cells and plasmatocytes showed unexpected differences with respect to properties of the plasma membrane and labeling with annexin V. Both permeabilized cells and plasmatocytes failed to show measurable mitochondrial membrane potential by flow cytometry using the JC-1 probe. Our results suggest hemolymph particulate populations are dynamic, revealing significant differences when comparing individual hive members, and when comparing colonies exposed to diverse conditions. Shifts in hemocyte populations in bees likely represent changing conditions or metabolic differences of colony members. A better understanding of hemocyte profiles may provide insight into physiological responses of honey bees to stress factors, some of which may be related to colony failure. |
| first_indexed | 2024-12-21T20:40:30Z |
| format | Article |
| id | doaj.art-c21be37eebe842c593a36eb08ac7e83b |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-21T20:40:30Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-c21be37eebe842c593a36eb08ac7e83b2022-12-21T18:51:00ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-01910e10848610.1371/journal.pone.0108486Honey bee hemocyte profiling by flow cytometry.William J MarringaMichael J KruegerNancy L BurrittJames B BurrittMultiple stress factors in honey bees are causing loss of bee colonies worldwide. Several infectious agents of bees are believed to contribute to this problem. The mechanisms of honey bee immunity are not completely understood, in part due to limited information about the types and abundances of hemocytes that help bees resist disease. Our study utilized flow cytometry and microscopy to examine populations of hemolymph particulates in honey bees. We found bee hemolymph includes permeabilized cells, plasmatocytes, and acellular objects that resemble microparticles, listed in order of increasing abundance. The permeabilized cells and plasmatocytes showed unexpected differences with respect to properties of the plasma membrane and labeling with annexin V. Both permeabilized cells and plasmatocytes failed to show measurable mitochondrial membrane potential by flow cytometry using the JC-1 probe. Our results suggest hemolymph particulate populations are dynamic, revealing significant differences when comparing individual hive members, and when comparing colonies exposed to diverse conditions. Shifts in hemocyte populations in bees likely represent changing conditions or metabolic differences of colony members. A better understanding of hemocyte profiles may provide insight into physiological responses of honey bees to stress factors, some of which may be related to colony failure.http://europepmc.org/articles/PMC4186811?pdf=render |
| spellingShingle | William J Marringa Michael J Krueger Nancy L Burritt James B Burritt Honey bee hemocyte profiling by flow cytometry. PLoS ONE |
| title | Honey bee hemocyte profiling by flow cytometry. |
| title_full | Honey bee hemocyte profiling by flow cytometry. |
| title_fullStr | Honey bee hemocyte profiling by flow cytometry. |
| title_full_unstemmed | Honey bee hemocyte profiling by flow cytometry. |
| title_short | Honey bee hemocyte profiling by flow cytometry. |
| title_sort | honey bee hemocyte profiling by flow cytometry |
| url | http://europepmc.org/articles/PMC4186811?pdf=render |
| work_keys_str_mv | AT williamjmarringa honeybeehemocyteprofilingbyflowcytometry AT michaeljkrueger honeybeehemocyteprofilingbyflowcytometry AT nancylburritt honeybeehemocyteprofilingbyflowcytometry AT jamesbburritt honeybeehemocyteprofilingbyflowcytometry |