MicroRNA Techniques: Valuable Tools for Agronomic Trait Analyses and Breeding in Rice
MicroRNAs (miRNAs) are a class of small noncoding RNAs that regulate gene expression at the post-transcriptional level. Extensive studies have revealed that miRNAs have critical functions in plant growth, development, and stress responses and may provide valuable genetic resources for plant breeding...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-09-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2021.744357/full |
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| author | Jiwei Chen Jiwei Chen Sachin Teotia Ting Lan Ting Lan Guiliang Tang |
| author_facet | Jiwei Chen Jiwei Chen Sachin Teotia Ting Lan Ting Lan Guiliang Tang |
| author_sort | Jiwei Chen |
| collection | DOAJ |
| description | MicroRNAs (miRNAs) are a class of small noncoding RNAs that regulate gene expression at the post-transcriptional level. Extensive studies have revealed that miRNAs have critical functions in plant growth, development, and stress responses and may provide valuable genetic resources for plant breeding research. We herein reviewed the development, mechanisms, and characteristics of miRNA techniques while highlighting widely used approaches, namely, the short tandem target mimic (STTM) approach. We described STTM-based advances in plant science, especially in the model crop rice, and introduced the CRISPR-based transgene-free crop breeding. Finally, we discussed the challenges and unique opportunities related to combining STTM and CRISPR technology for crop improvement and agriculture. |
| first_indexed | 2024-12-17T19:50:20Z |
| format | Article |
| id | doaj.art-c21d03a001364f5cb69ca940b2e6883e |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-17T19:50:20Z |
| publishDate | 2021-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-c21d03a001364f5cb69ca940b2e6883e2022-12-21T21:34:44ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2021-09-011210.3389/fpls.2021.744357744357MicroRNA Techniques: Valuable Tools for Agronomic Trait Analyses and Breeding in RiceJiwei Chen0Jiwei Chen1Sachin Teotia2Ting Lan3Ting Lan4Guiliang Tang5Guangdong Provincial Key Laboratory for Plant Epigenetics, Longhua Bioindustry and Innovation Research Institute, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, ChinaKey Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Optoelectronic Engineering, Shenzhen University, Shenzhen, ChinaDepartment of Biotechnology, Sharda University, Greater Noida, IndiaGuangdong Provincial Key Laboratory for Plant Epigenetics, Longhua Bioindustry and Innovation Research Institute, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, ChinaKey Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Optoelectronic Engineering, Shenzhen University, Shenzhen, ChinaDepartment of Biological Sciences, Life Science and Technology Institute, Michigan Technological University, Houghton, MI, United StatesMicroRNAs (miRNAs) are a class of small noncoding RNAs that regulate gene expression at the post-transcriptional level. Extensive studies have revealed that miRNAs have critical functions in plant growth, development, and stress responses and may provide valuable genetic resources for plant breeding research. We herein reviewed the development, mechanisms, and characteristics of miRNA techniques while highlighting widely used approaches, namely, the short tandem target mimic (STTM) approach. We described STTM-based advances in plant science, especially in the model crop rice, and introduced the CRISPR-based transgene-free crop breeding. Finally, we discussed the challenges and unique opportunities related to combining STTM and CRISPR technology for crop improvement and agriculture.https://www.frontiersin.org/articles/10.3389/fpls.2021.744357/fullmicroRNAshort tandem target mimicagronomic traitscrop breedingCRISPR/Cas9 |
| spellingShingle | Jiwei Chen Jiwei Chen Sachin Teotia Ting Lan Ting Lan Guiliang Tang MicroRNA Techniques: Valuable Tools for Agronomic Trait Analyses and Breeding in Rice Frontiers in Plant Science microRNA short tandem target mimic agronomic traits crop breeding CRISPR/Cas9 |
| title | MicroRNA Techniques: Valuable Tools for Agronomic Trait Analyses and Breeding in Rice |
| title_full | MicroRNA Techniques: Valuable Tools for Agronomic Trait Analyses and Breeding in Rice |
| title_fullStr | MicroRNA Techniques: Valuable Tools for Agronomic Trait Analyses and Breeding in Rice |
| title_full_unstemmed | MicroRNA Techniques: Valuable Tools for Agronomic Trait Analyses and Breeding in Rice |
| title_short | MicroRNA Techniques: Valuable Tools for Agronomic Trait Analyses and Breeding in Rice |
| title_sort | microrna techniques valuable tools for agronomic trait analyses and breeding in rice |
| topic | microRNA short tandem target mimic agronomic traits crop breeding CRISPR/Cas9 |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2021.744357/full |
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