Structural Characterization of a Polysaccharide from <i>Gastrodia elata</i> and Its Bioactivity on Gut Microbiota
A novel homogeneous polysaccharide named <b>GEP-1</b> was isolated and purified from <i>Gastrodia elata</i> (<i>G. elata</i>) by hot-water extraction, ethanol precipitation, and membrane separator. <b>GEP-1</b>, which has a molecular weight of 20.1 kDa...
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MDPI AG
2021-07-01
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| Online Access: | https://www.mdpi.com/1420-3049/26/15/4443 |
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| author | Jiangyan Huo Min Lei Feifei Li Jinjun Hou Zijia Zhang Huali Long Xianchun Zhong Yameng Liu Cen Xie Wanying Wu |
| author_facet | Jiangyan Huo Min Lei Feifei Li Jinjun Hou Zijia Zhang Huali Long Xianchun Zhong Yameng Liu Cen Xie Wanying Wu |
| author_sort | Jiangyan Huo |
| collection | DOAJ |
| description | A novel homogeneous polysaccharide named <b>GEP-1</b> was isolated and purified from <i>Gastrodia elata</i> (<i>G. elata</i>) by hot-water extraction, ethanol precipitation, and membrane separator. <b>GEP-1</b>, which has a molecular weight of 20.1 kDa, contains a polysaccharide framework comprised of only glucose. Methylation and NMR analysis showed that <b>GEP-1</b> contained 1,3,6-linked-α-Glc<i>p</i>, 1,4-linked-α-Glc<i>p</i>, 1,4-linked-β-Glc<i>p</i> and 1,4,6-linked-α-Glc<i>p</i>. Interestingly, <b>GEP-1</b> contained citric acid and repeating <i>p</i>-hydroxybenzyl alcohol as one branch. Furthermore, a bioactivity test showed that <b>GEP-1</b> could significantly promote the growth of <i>Akkermansia muciniphila</i> (<i>A. muciniphila</i>) and <i>Lacticaseibacillus paracasei</i> (<i>L.</i><i>paracasei</i>) strains. These results implied that <b>GEP-1</b> might be useful for human by modulating gut microbiota. |
| first_indexed | 2024-03-10T09:11:25Z |
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| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T09:11:25Z |
| publishDate | 2021-07-01 |
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| series | Molecules |
| spelling | doaj.art-c21d4af1efbb43ce8d9cb84ffce4b3e72023-11-22T05:58:18ZengMDPI AGMolecules1420-30492021-07-012615444310.3390/molecules26154443Structural Characterization of a Polysaccharide from <i>Gastrodia elata</i> and Its Bioactivity on Gut MicrobiotaJiangyan Huo0Min Lei1Feifei Li2Jinjun Hou3Zijia Zhang4Huali Long5Xianchun Zhong6Yameng Liu7Cen Xie8Wanying Wu9Shanghai Research Center for Modernization of Traditional Chinese Medicine, National Engineering Laboratory for TCM Standardization Technology, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, ChinaShanghai Research Center for Modernization of Traditional Chinese Medicine, National Engineering Laboratory for TCM Standardization Technology, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, ChinaShanghai Research Center for Modernization of Traditional Chinese Medicine, National Engineering Laboratory for TCM Standardization Technology, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, ChinaShanghai Research Center for Modernization of Traditional Chinese Medicine, National Engineering Laboratory for TCM Standardization Technology, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, ChinaShanghai Research Center for Modernization of Traditional Chinese Medicine, National Engineering Laboratory for TCM Standardization Technology, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, ChinaShanghai Research Center for Modernization of Traditional Chinese Medicine, National Engineering Laboratory for TCM Standardization Technology, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, ChinaShanghai Research Center for Modernization of Traditional Chinese Medicine, National Engineering Laboratory for TCM Standardization Technology, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, ChinaShanghai Research Center for Modernization of Traditional Chinese Medicine, National Engineering Laboratory for TCM Standardization Technology, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, ChinaShanghai Research Center for Modernization of Traditional Chinese Medicine, National Engineering Laboratory for TCM Standardization Technology, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, ChinaShanghai Research Center for Modernization of Traditional Chinese Medicine, National Engineering Laboratory for TCM Standardization Technology, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, ChinaA novel homogeneous polysaccharide named <b>GEP-1</b> was isolated and purified from <i>Gastrodia elata</i> (<i>G. elata</i>) by hot-water extraction, ethanol precipitation, and membrane separator. <b>GEP-1</b>, which has a molecular weight of 20.1 kDa, contains a polysaccharide framework comprised of only glucose. Methylation and NMR analysis showed that <b>GEP-1</b> contained 1,3,6-linked-α-Glc<i>p</i>, 1,4-linked-α-Glc<i>p</i>, 1,4-linked-β-Glc<i>p</i> and 1,4,6-linked-α-Glc<i>p</i>. Interestingly, <b>GEP-1</b> contained citric acid and repeating <i>p</i>-hydroxybenzyl alcohol as one branch. Furthermore, a bioactivity test showed that <b>GEP-1</b> could significantly promote the growth of <i>Akkermansia muciniphila</i> (<i>A. muciniphila</i>) and <i>Lacticaseibacillus paracasei</i> (<i>L.</i><i>paracasei</i>) strains. These results implied that <b>GEP-1</b> might be useful for human by modulating gut microbiota.https://www.mdpi.com/1420-3049/26/15/4443<i>Gastrodia elata</i>structural characterizationpolysaccharidegut microbiota |
| spellingShingle | Jiangyan Huo Min Lei Feifei Li Jinjun Hou Zijia Zhang Huali Long Xianchun Zhong Yameng Liu Cen Xie Wanying Wu Structural Characterization of a Polysaccharide from <i>Gastrodia elata</i> and Its Bioactivity on Gut Microbiota Molecules <i>Gastrodia elata</i> structural characterization polysaccharide gut microbiota |
| title | Structural Characterization of a Polysaccharide from <i>Gastrodia elata</i> and Its Bioactivity on Gut Microbiota |
| title_full | Structural Characterization of a Polysaccharide from <i>Gastrodia elata</i> and Its Bioactivity on Gut Microbiota |
| title_fullStr | Structural Characterization of a Polysaccharide from <i>Gastrodia elata</i> and Its Bioactivity on Gut Microbiota |
| title_full_unstemmed | Structural Characterization of a Polysaccharide from <i>Gastrodia elata</i> and Its Bioactivity on Gut Microbiota |
| title_short | Structural Characterization of a Polysaccharide from <i>Gastrodia elata</i> and Its Bioactivity on Gut Microbiota |
| title_sort | structural characterization of a polysaccharide from i gastrodia elata i and its bioactivity on gut microbiota |
| topic | <i>Gastrodia elata</i> structural characterization polysaccharide gut microbiota |
| url | https://www.mdpi.com/1420-3049/26/15/4443 |
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