Enhanced Nicotiana benthamiana immune responses caused by heterologous plant genes from Pinellia ternata
Abstract Background Pinellia ternata is a Chinese traditional medicinal herb, used to cure diseases including insomnia, eclampsia and cervical carcinoma, for hundreds of years. Non-self-recognition in multicellular organisms can initiate the innate immunity to avoid the invasion of pathogens. A desi...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2018-12-01
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| Series: | BMC Plant Biology |
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| Online Access: | http://link.springer.com/article/10.1186/s12870-018-1598-5 |
| _version_ | 1818096590117666816 |
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| author | Hafiz Muhammad Khalid Abbas Jingshu Xiang Zahoor Ahmad Lilin Wang Wubei Dong |
| author_facet | Hafiz Muhammad Khalid Abbas Jingshu Xiang Zahoor Ahmad Lilin Wang Wubei Dong |
| author_sort | Hafiz Muhammad Khalid Abbas |
| collection | DOAJ |
| description | Abstract Background Pinellia ternata is a Chinese traditional medicinal herb, used to cure diseases including insomnia, eclampsia and cervical carcinoma, for hundreds of years. Non-self-recognition in multicellular organisms can initiate the innate immunity to avoid the invasion of pathogens. A design for pathogen independent, heterosis based, fresh resistance can be generated in F1 hybrid was proposed. Results By library functional screening, we found that P. ternata genes, named as ptHR375 and ptHR941, were identified with the potential to trigger a hypersensitive response in Nicotiana benthamiana. Significant induction of ROS and Callose deposition in N. benthamiana leaves along with activation of pathogenesis-related genes viz.; PR-1a, PR-5, PDF1.2, NPR1, PAL, RBOHB and ERF1 and antioxidant enzymes was observed. After transformation into N. benthamiana, expression of pathogenesis related genes was significantly up-regulated to generate high level of resistance against Phytophthora capsici without affecting the normal seed germination and morphological characters of the transformed N. benthamiana. UPLC-QTOF-MS analysis of ptHR375 transformed N. benthamiana revealed the induction of Oxytetracycline, Cuelure, Allantoin, Diethylstilbestrol and 1,2-Benzisothiazol-3(2H)-one as bioactive compounds. Here we also proved that F1 hybrids, produced by crossing of the ptHR375 and ptHR941 transformed and non-transformed N. benthamiana, show significant high levels of PR-gene expressions and pathogen resistance. Conclusions Heterologous plant genes can activate disease resistance in another plant species and furthermore, by generating F1 hybrids, fresh pathogen independent plant immunity can be obtained. It is also concluded that ptHR375 and ptHR941 play their role in SA and JA/ET defense pathways to activate the resistance against invading pathogens. |
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| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-12-10T23:07:02Z |
| publishDate | 2018-12-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-c21e50c60b4a4066999fbc60b5d6b82b2022-12-22T01:30:02ZengBMCBMC Plant Biology1471-22292018-12-0118111410.1186/s12870-018-1598-5Enhanced Nicotiana benthamiana immune responses caused by heterologous plant genes from Pinellia ternataHafiz Muhammad Khalid Abbas0Jingshu Xiang1Zahoor Ahmad2Lilin Wang3Wubei Dong4Department of Plant Pathology, College of Plant Science and Technology and the Key Lab of Crop Disease Monitoring & Safety Control in Hubei Province, Huazhong Agricultural UniversityDepartment of Plant Pathology, College of Plant Science and Technology and the Key Lab of Crop Disease Monitoring & Safety Control in Hubei Province, Huazhong Agricultural UniversityDepartment of Plant Pathology, College of Plant Science and Technology and the Key Lab of Crop Disease Monitoring & Safety Control in Hubei Province, Huazhong Agricultural UniversityDepartment of Plant Pathology, College of Plant Science and Technology and the Key Lab of Crop Disease Monitoring & Safety Control in Hubei Province, Huazhong Agricultural UniversityDepartment of Plant Pathology, College of Plant Science and Technology and the Key Lab of Crop Disease Monitoring & Safety Control in Hubei Province, Huazhong Agricultural UniversityAbstract Background Pinellia ternata is a Chinese traditional medicinal herb, used to cure diseases including insomnia, eclampsia and cervical carcinoma, for hundreds of years. Non-self-recognition in multicellular organisms can initiate the innate immunity to avoid the invasion of pathogens. A design for pathogen independent, heterosis based, fresh resistance can be generated in F1 hybrid was proposed. Results By library functional screening, we found that P. ternata genes, named as ptHR375 and ptHR941, were identified with the potential to trigger a hypersensitive response in Nicotiana benthamiana. Significant induction of ROS and Callose deposition in N. benthamiana leaves along with activation of pathogenesis-related genes viz.; PR-1a, PR-5, PDF1.2, NPR1, PAL, RBOHB and ERF1 and antioxidant enzymes was observed. After transformation into N. benthamiana, expression of pathogenesis related genes was significantly up-regulated to generate high level of resistance against Phytophthora capsici without affecting the normal seed germination and morphological characters of the transformed N. benthamiana. UPLC-QTOF-MS analysis of ptHR375 transformed N. benthamiana revealed the induction of Oxytetracycline, Cuelure, Allantoin, Diethylstilbestrol and 1,2-Benzisothiazol-3(2H)-one as bioactive compounds. Here we also proved that F1 hybrids, produced by crossing of the ptHR375 and ptHR941 transformed and non-transformed N. benthamiana, show significant high levels of PR-gene expressions and pathogen resistance. Conclusions Heterologous plant genes can activate disease resistance in another plant species and furthermore, by generating F1 hybrids, fresh pathogen independent plant immunity can be obtained. It is also concluded that ptHR375 and ptHR941 play their role in SA and JA/ET defense pathways to activate the resistance against invading pathogens.http://link.springer.com/article/10.1186/s12870-018-1598-5Durable disease resistanceHypersensitive responseNicotiana benthamiana; non-self-recognitionPathogenesis related genesPinellia ternata |
| spellingShingle | Hafiz Muhammad Khalid Abbas Jingshu Xiang Zahoor Ahmad Lilin Wang Wubei Dong Enhanced Nicotiana benthamiana immune responses caused by heterologous plant genes from Pinellia ternata BMC Plant Biology Durable disease resistance Hypersensitive response Nicotiana benthamiana; non-self-recognition Pathogenesis related genes Pinellia ternata |
| title | Enhanced Nicotiana benthamiana immune responses caused by heterologous plant genes from Pinellia ternata |
| title_full | Enhanced Nicotiana benthamiana immune responses caused by heterologous plant genes from Pinellia ternata |
| title_fullStr | Enhanced Nicotiana benthamiana immune responses caused by heterologous plant genes from Pinellia ternata |
| title_full_unstemmed | Enhanced Nicotiana benthamiana immune responses caused by heterologous plant genes from Pinellia ternata |
| title_short | Enhanced Nicotiana benthamiana immune responses caused by heterologous plant genes from Pinellia ternata |
| title_sort | enhanced nicotiana benthamiana immune responses caused by heterologous plant genes from pinellia ternata |
| topic | Durable disease resistance Hypersensitive response Nicotiana benthamiana; non-self-recognition Pathogenesis related genes Pinellia ternata |
| url | http://link.springer.com/article/10.1186/s12870-018-1598-5 |
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