The Function of Ion Channels and Membrane Potential in Red Blood Cells: Toward a Systematic Analysis of the Erythroid Channelome
Erythrocytes represent at least 60% of all cells in the human body. During circulation, they experience a huge variety of physical and chemical stimulations, such as pressure, shear stress, hormones or osmolarity changes. These signals are translated into cellular responses through ion channels that...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-02-01
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| Series: | Frontiers in Physiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphys.2022.824478/full |
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| author | Marieke von Lindern Marieke von Lindern Stéphane Egée Stéphane Egée Paola Bianchi Lars Kaestner Lars Kaestner |
| author_facet | Marieke von Lindern Marieke von Lindern Stéphane Egée Stéphane Egée Paola Bianchi Lars Kaestner Lars Kaestner |
| author_sort | Marieke von Lindern |
| collection | DOAJ |
| description | Erythrocytes represent at least 60% of all cells in the human body. During circulation, they experience a huge variety of physical and chemical stimulations, such as pressure, shear stress, hormones or osmolarity changes. These signals are translated into cellular responses through ion channels that modulate erythrocyte function. Ion channels in erythrocytes are only recently recognized as utmost important players in physiology and pathophysiology. Despite this awareness, their signaling, interactions and concerted regulation, such as the generation and effects of “pseudo action potentials”, remain elusive. We propose a systematic, conjoined approach using molecular biology, in vitro erythropoiesis, state-of-the-art electrophysiological techniques, and channelopathy patient samples to decipher the role of ion channel functions in health and disease. We need to overcome challenges such as the heterogeneity of the cell population (120 days lifespan without protein renewal) or the access to large cohorts of patients. Thereto we will use genetic manipulation of progenitors, cell differentiation into erythrocytes, and statistically efficient electrophysiological recordings of ion channel activity. |
| first_indexed | 2024-12-24T23:03:06Z |
| format | Article |
| id | doaj.art-c22a04b8a9924e38a17c2ddb8367d489 |
| institution | Directory Open Access Journal |
| issn | 1664-042X |
| language | English |
| last_indexed | 2024-12-24T23:03:06Z |
| publishDate | 2022-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Physiology |
| spelling | doaj.art-c22a04b8a9924e38a17c2ddb8367d4892022-12-21T16:35:05ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2022-02-011310.3389/fphys.2022.824478824478The Function of Ion Channels and Membrane Potential in Red Blood Cells: Toward a Systematic Analysis of the Erythroid ChannelomeMarieke von Lindern0Marieke von Lindern1Stéphane Egée2Stéphane Egée3Paola Bianchi4Lars Kaestner5Lars Kaestner6Sanquin Research and Landsteiner Laboratory, Department of Hematopoiesis, Amsterdam UMC, University of Amsterdam, Amsterdam, NetherlandsDepartment of Cell Biology and Genetics, Erasmus University Medical Center, Rotterdam, NetherlandsIntegrative Biology of Marine Models, Station Biologique de Roscoff, CNRS, UMR 8227, Sorbonne Université, Roscoff Cedex, FranceLaboratoire d’Excellence GR-Ex, Paris, FrancePathophysiology of Anemia Unit, Hematology Unit, Fondazione IRCCS Ca’ Granda Ospedale Maggiore Policlinico of Milan, Milan, ItalyTheoretical Medicine and Biosciences, Medical Faculty, Saarland University, Homburg, GermanyDynamics of Fluids, Experimental Physics, Saarland University, Saarbrücken, GermanyErythrocytes represent at least 60% of all cells in the human body. During circulation, they experience a huge variety of physical and chemical stimulations, such as pressure, shear stress, hormones or osmolarity changes. These signals are translated into cellular responses through ion channels that modulate erythrocyte function. Ion channels in erythrocytes are only recently recognized as utmost important players in physiology and pathophysiology. Despite this awareness, their signaling, interactions and concerted regulation, such as the generation and effects of “pseudo action potentials”, remain elusive. We propose a systematic, conjoined approach using molecular biology, in vitro erythropoiesis, state-of-the-art electrophysiological techniques, and channelopathy patient samples to decipher the role of ion channel functions in health and disease. We need to overcome challenges such as the heterogeneity of the cell population (120 days lifespan without protein renewal) or the access to large cohorts of patients. Thereto we will use genetic manipulation of progenitors, cell differentiation into erythrocytes, and statistically efficient electrophysiological recordings of ion channel activity.https://www.frontiersin.org/articles/10.3389/fphys.2022.824478/fullerythrocyteerythropoiesispatch-clampion channelelectrophysiologychannelopathy |
| spellingShingle | Marieke von Lindern Marieke von Lindern Stéphane Egée Stéphane Egée Paola Bianchi Lars Kaestner Lars Kaestner The Function of Ion Channels and Membrane Potential in Red Blood Cells: Toward a Systematic Analysis of the Erythroid Channelome Frontiers in Physiology erythrocyte erythropoiesis patch-clamp ion channel electrophysiology channelopathy |
| title | The Function of Ion Channels and Membrane Potential in Red Blood Cells: Toward a Systematic Analysis of the Erythroid Channelome |
| title_full | The Function of Ion Channels and Membrane Potential in Red Blood Cells: Toward a Systematic Analysis of the Erythroid Channelome |
| title_fullStr | The Function of Ion Channels and Membrane Potential in Red Blood Cells: Toward a Systematic Analysis of the Erythroid Channelome |
| title_full_unstemmed | The Function of Ion Channels and Membrane Potential in Red Blood Cells: Toward a Systematic Analysis of the Erythroid Channelome |
| title_short | The Function of Ion Channels and Membrane Potential in Red Blood Cells: Toward a Systematic Analysis of the Erythroid Channelome |
| title_sort | function of ion channels and membrane potential in red blood cells toward a systematic analysis of the erythroid channelome |
| topic | erythrocyte erythropoiesis patch-clamp ion channel electrophysiology channelopathy |
| url | https://www.frontiersin.org/articles/10.3389/fphys.2022.824478/full |
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