Metabolomic Changes in Sogatella furcifera under Southern rice black-streaked dwarf virus Infection and Temperature Stress
Southern rice black-streaked dwarf virus (SRBSDV) is a devastating newly emerged rice reovirus in Eastern and Southeastern Asia transmitted by a long-distance migratory pest, the white-backed planthopper (WBPH). We previously showed that SRBSDV infection decreased the cold tolerance but improved the...
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| Format: | Article |
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MDPI AG
2018-06-01
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| Series: | Viruses |
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| Online Access: | http://www.mdpi.com/1999-4915/10/7/344 |
| _version_ | 1819263395181887488 |
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| author | Tong Zhang Wendi Feng Jiajie Ye Zhanbiao Li Guohui Zhou |
| author_facet | Tong Zhang Wendi Feng Jiajie Ye Zhanbiao Li Guohui Zhou |
| author_sort | Tong Zhang |
| collection | DOAJ |
| description | Southern rice black-streaked dwarf virus (SRBSDV) is a devastating newly emerged rice reovirus in Eastern and Southeastern Asia transmitted by a long-distance migratory pest, the white-backed planthopper (WBPH). We previously showed that SRBSDV infection decreased the cold tolerance but improved the heat tolerance of its vector, WBPH. Comparative metabolomic analysis was used to explore the potential mechanisms underlying these changes in temperature stress response. Fourth-generation WBPH nymphs were treated with SRBSDV and/or extreme temperature stress and were analyzed using gas chromatography-time of flight-mass spectrometry. A total of 605 distinguishable peaks were identified and 165, 207, and 202 differentially accumulated metabolites were identified in WBPH after virus infection, cold, or heat stress, respectively. The nucleic acids and fatty acids were the major categories of metabolites regulated by SRBSDV infection, whereas temperature stress regulated tricarboxylic acid cycle compounds, sugars, and polyols. For the WBPH samples infected with SRBSDV and subjected to temperature stress, amino acids, sugars, and polyols were the most significant regulated metabolites. The metabolomics study suggests that SRBSDV may influence the extreme temperature tolerance of WBPH by regulating the accumulation of amino acids, sugars, and polyols in the insect body. |
| first_indexed | 2024-12-23T20:12:54Z |
| format | Article |
| id | doaj.art-c22c44ef67604c5f84d1c21f8d7ab6b0 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-12-23T20:12:54Z |
| publishDate | 2018-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-c22c44ef67604c5f84d1c21f8d7ab6b02022-12-21T17:32:47ZengMDPI AGViruses1999-49152018-06-0110734410.3390/v10070344v10070344Metabolomic Changes in Sogatella furcifera under Southern rice black-streaked dwarf virus Infection and Temperature StressTong Zhang0Wendi Feng1Jiajie Ye2Zhanbiao Li3Guohui Zhou4Guangdong Province Key Laboratory of Microbial Signals and Disease Control, College of Agriculture, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Province Key Laboratory of Microbial Signals and Disease Control, College of Agriculture, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Province Key Laboratory of Microbial Signals and Disease Control, College of Agriculture, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Province Key Laboratory of Microbial Signals and Disease Control, College of Agriculture, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Province Key Laboratory of Microbial Signals and Disease Control, College of Agriculture, South China Agricultural University, Guangzhou 510642, ChinaSouthern rice black-streaked dwarf virus (SRBSDV) is a devastating newly emerged rice reovirus in Eastern and Southeastern Asia transmitted by a long-distance migratory pest, the white-backed planthopper (WBPH). We previously showed that SRBSDV infection decreased the cold tolerance but improved the heat tolerance of its vector, WBPH. Comparative metabolomic analysis was used to explore the potential mechanisms underlying these changes in temperature stress response. Fourth-generation WBPH nymphs were treated with SRBSDV and/or extreme temperature stress and were analyzed using gas chromatography-time of flight-mass spectrometry. A total of 605 distinguishable peaks were identified and 165, 207, and 202 differentially accumulated metabolites were identified in WBPH after virus infection, cold, or heat stress, respectively. The nucleic acids and fatty acids were the major categories of metabolites regulated by SRBSDV infection, whereas temperature stress regulated tricarboxylic acid cycle compounds, sugars, and polyols. For the WBPH samples infected with SRBSDV and subjected to temperature stress, amino acids, sugars, and polyols were the most significant regulated metabolites. The metabolomics study suggests that SRBSDV may influence the extreme temperature tolerance of WBPH by regulating the accumulation of amino acids, sugars, and polyols in the insect body.http://www.mdpi.com/1999-4915/10/7/344white-backed planthopper (WBPH)Southern rice black-streaked dwarf virus (SRBSDV)temperature stressgas chromatography-time of flight-mass spectrometry (GC-TOF-MS)differential metabolite accumulation |
| spellingShingle | Tong Zhang Wendi Feng Jiajie Ye Zhanbiao Li Guohui Zhou Metabolomic Changes in Sogatella furcifera under Southern rice black-streaked dwarf virus Infection and Temperature Stress Viruses white-backed planthopper (WBPH) Southern rice black-streaked dwarf virus (SRBSDV) temperature stress gas chromatography-time of flight-mass spectrometry (GC-TOF-MS) differential metabolite accumulation |
| title | Metabolomic Changes in Sogatella furcifera under Southern rice black-streaked dwarf virus Infection and Temperature Stress |
| title_full | Metabolomic Changes in Sogatella furcifera under Southern rice black-streaked dwarf virus Infection and Temperature Stress |
| title_fullStr | Metabolomic Changes in Sogatella furcifera under Southern rice black-streaked dwarf virus Infection and Temperature Stress |
| title_full_unstemmed | Metabolomic Changes in Sogatella furcifera under Southern rice black-streaked dwarf virus Infection and Temperature Stress |
| title_short | Metabolomic Changes in Sogatella furcifera under Southern rice black-streaked dwarf virus Infection and Temperature Stress |
| title_sort | metabolomic changes in sogatella furcifera under southern rice black streaked dwarf virus infection and temperature stress |
| topic | white-backed planthopper (WBPH) Southern rice black-streaked dwarf virus (SRBSDV) temperature stress gas chromatography-time of flight-mass spectrometry (GC-TOF-MS) differential metabolite accumulation |
| url | http://www.mdpi.com/1999-4915/10/7/344 |
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