| Summary: | The redox reaction is a normal process of biological metabolism in the body that leads to the production of free radicals. Under conditions such as pathogenic infection, stress, and drug exposure, free radicals can exceed normal levels, causing protein denaturation, DNA damage, and the oxidation of the cell membrane, which, in turn, causes inflammation. <i>Acanthopanax senticosus (A. senticosus)</i> flavonoids are the main bioactive ingredients with antioxidant function. H<sub>2</sub>O<sub>2</sub>-treated RAW 264.7 cells and DSS-induced colitis in mice were used to evaluate the antioxidant properties of <i>A. senticosus</i> flavonoids. The results show that <i>A. senticosus</i> flavonoids can significantly downregulate the levels of ROS and MDA in H<sub>2</sub>O<sub>2</sub>-treated RAW 264.7 cells and increase the levels of CAT, SOD, and GPx. <i>A. senticosus</i> flavonoids can also increase the body weights of DSS-induced colitis mice, increase the DAI index, and ameliorate the shortening of the colon. ELISA experiments confirmed that <i>A. senticosus</i> flavonoids could reduce the level of MDA in the mouse serum and increase the levels of SOD, CAT, and GPx. Histopathology showed that the tissue pathological changes in the <i>A. senticosus</i> flavonoid group were significantly lower than those in the DSS group. The Western blot experiments showed that the antioxidant capacity of <i>A. senticosus</i> flavonoids was accomplished through the Nrf2 pathway. In conclusion, <i>A. senticosus</i> flavonoids can relieve oxidative stress in vivo and in vitro and protect cells or tissues from oxidative damage.
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