Unravelling the Mystery inside Cells by Using Single-Molecule Fluorescence Imaging
Live-cell imaging is a powerful technique to study the dynamics and mechanics of various biological molecules like proteins, organelles, DNA, and RNA. With the rapid evolution of optical microscopy, our understanding of how these molecules are implicated in the cells’ most critical physiological rol...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-09-01
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| Series: | Journal of Imaging |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2313-433X/9/9/192 |
| _version_ | 1797579396469489664 |
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| author | Julian Zalejski Jiachen Sun Ashutosh Sharma |
| author_facet | Julian Zalejski Jiachen Sun Ashutosh Sharma |
| author_sort | Julian Zalejski |
| collection | DOAJ |
| description | Live-cell imaging is a powerful technique to study the dynamics and mechanics of various biological molecules like proteins, organelles, DNA, and RNA. With the rapid evolution of optical microscopy, our understanding of how these molecules are implicated in the cells’ most critical physiological roles deepens. In this review, we focus on how spatiotemporal nanoscale live-cell imaging at the single molecule level allows for profound contributions towards new discoveries in life science. This review will start by summarizing how single-molecule tracking has been used to analyze membrane dynamics, receptor–ligand interactions, protein–protein interactions, inner- and extra-cellular transport, gene expression/transcription, and whole organelle tracking. We then move on to how current authors are trying to improve single-molecule tracking and overcome current limitations by offering new ways of labeling proteins of interest, multi-channel/color detection, improvements in time-lapse imaging, and new methods and programs to analyze the colocalization and movement of targets. We later discuss how single-molecule tracking can be a beneficial tool used for medical diagnosis. Finally, we wrap up with the limitations and future perspectives of single-molecule tracking and total internal reflection microscopy. |
| first_indexed | 2024-03-10T22:36:32Z |
| format | Article |
| id | doaj.art-c230d3a8c8974abca34aa99ecd1e6f9a |
| institution | Directory Open Access Journal |
| issn | 2313-433X |
| language | English |
| last_indexed | 2024-03-10T22:36:32Z |
| publishDate | 2023-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Imaging |
| spelling | doaj.art-c230d3a8c8974abca34aa99ecd1e6f9a2023-11-19T11:24:51ZengMDPI AGJournal of Imaging2313-433X2023-09-019919210.3390/jimaging9090192Unravelling the Mystery inside Cells by Using Single-Molecule Fluorescence ImagingJulian Zalejski0Jiachen Sun1Ashutosh Sharma2Department of Chemistry, University of Illinois Chicago, Chicago, IL 60607, USADepartment of Chemistry, University of Illinois Chicago, Chicago, IL 60607, USADepartment of Chemistry, University of Illinois Chicago, Chicago, IL 60607, USALive-cell imaging is a powerful technique to study the dynamics and mechanics of various biological molecules like proteins, organelles, DNA, and RNA. With the rapid evolution of optical microscopy, our understanding of how these molecules are implicated in the cells’ most critical physiological roles deepens. In this review, we focus on how spatiotemporal nanoscale live-cell imaging at the single molecule level allows for profound contributions towards new discoveries in life science. This review will start by summarizing how single-molecule tracking has been used to analyze membrane dynamics, receptor–ligand interactions, protein–protein interactions, inner- and extra-cellular transport, gene expression/transcription, and whole organelle tracking. We then move on to how current authors are trying to improve single-molecule tracking and overcome current limitations by offering new ways of labeling proteins of interest, multi-channel/color detection, improvements in time-lapse imaging, and new methods and programs to analyze the colocalization and movement of targets. We later discuss how single-molecule tracking can be a beneficial tool used for medical diagnosis. Finally, we wrap up with the limitations and future perspectives of single-molecule tracking and total internal reflection microscopy.https://www.mdpi.com/2313-433X/9/9/192protein–protein interactionmedical diagnosticscell imaging and signalingtotal internal reflection fluorescenceprotein dynamics |
| spellingShingle | Julian Zalejski Jiachen Sun Ashutosh Sharma Unravelling the Mystery inside Cells by Using Single-Molecule Fluorescence Imaging Journal of Imaging protein–protein interaction medical diagnostics cell imaging and signaling total internal reflection fluorescence protein dynamics |
| title | Unravelling the Mystery inside Cells by Using Single-Molecule Fluorescence Imaging |
| title_full | Unravelling the Mystery inside Cells by Using Single-Molecule Fluorescence Imaging |
| title_fullStr | Unravelling the Mystery inside Cells by Using Single-Molecule Fluorescence Imaging |
| title_full_unstemmed | Unravelling the Mystery inside Cells by Using Single-Molecule Fluorescence Imaging |
| title_short | Unravelling the Mystery inside Cells by Using Single-Molecule Fluorescence Imaging |
| title_sort | unravelling the mystery inside cells by using single molecule fluorescence imaging |
| topic | protein–protein interaction medical diagnostics cell imaging and signaling total internal reflection fluorescence protein dynamics |
| url | https://www.mdpi.com/2313-433X/9/9/192 |
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