Infecting human hematopoietic stem and progenitor cells with SARS-CoV-2
Summary: Determining how hematopoietic stem and progenitor cells (HSPCs) can be infected by viruses is necessary to understand and predict how the immune system will drive the host response. We present here a protocol to analyze the capacity of SARS-CoV-2 to infect different subsets of human HSPCs,...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2021-12-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166721006092 |
| _version_ | 1819174574232698880 |
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| author | Hector Huerga Encabo Rachel Ulferts Aneesh Sharma Rupert Beale Dominique Bonnet |
| author_facet | Hector Huerga Encabo Rachel Ulferts Aneesh Sharma Rupert Beale Dominique Bonnet |
| author_sort | Hector Huerga Encabo |
| collection | DOAJ |
| description | Summary: Determining how hematopoietic stem and progenitor cells (HSPCs) can be infected by viruses is necessary to understand and predict how the immune system will drive the host response. We present here a protocol to analyze the capacity of SARS-CoV-2 to infect different subsets of human HSPCs, inlcuding procedures for SARS-CoV-2 production and titration, isolation of human HSPCs from different sources (bone marrow, umbilical cord, or peripheral blood), and quantification of SARS-Cov-2 infection capacity by RT-qPCR and colony forming unit assay.For complete details on the use and execution of this protocol, please refer to Huerga Encabo et al. (2021) |
| first_indexed | 2024-12-22T20:41:08Z |
| format | Article |
| id | doaj.art-c23222a5106946579e969b824591faf8 |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-12-22T20:41:08Z |
| publishDate | 2021-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-c23222a5106946579e969b824591faf82022-12-21T18:13:19ZengElsevierSTAR Protocols2666-16672021-12-0124100903Infecting human hematopoietic stem and progenitor cells with SARS-CoV-2Hector Huerga Encabo0Rachel Ulferts1Aneesh Sharma2Rupert Beale3Dominique Bonnet4Hematopoietic Stem Cell Laboratory, The Francis Crick Institute, London, NW1 1AT, UK; Corresponding authorCell Biology of Infection Laboratory, The Francis Crick Institute, London NW1 1AT, UK; Corresponding authorHematopoietic Stem Cell Laboratory, The Francis Crick Institute, London, NW1 1AT, UKCell Biology of Infection Laboratory, The Francis Crick Institute, London NW1 1AT, UKHematopoietic Stem Cell Laboratory, The Francis Crick Institute, London, NW1 1AT, UK; Corresponding authorSummary: Determining how hematopoietic stem and progenitor cells (HSPCs) can be infected by viruses is necessary to understand and predict how the immune system will drive the host response. We present here a protocol to analyze the capacity of SARS-CoV-2 to infect different subsets of human HSPCs, inlcuding procedures for SARS-CoV-2 production and titration, isolation of human HSPCs from different sources (bone marrow, umbilical cord, or peripheral blood), and quantification of SARS-Cov-2 infection capacity by RT-qPCR and colony forming unit assay.For complete details on the use and execution of this protocol, please refer to Huerga Encabo et al. (2021)http://www.sciencedirect.com/science/article/pii/S2666166721006092Cell BiologyCell isolationCell-based AssaysImmunologyMicrobiologyMicroscopy |
| spellingShingle | Hector Huerga Encabo Rachel Ulferts Aneesh Sharma Rupert Beale Dominique Bonnet Infecting human hematopoietic stem and progenitor cells with SARS-CoV-2 STAR Protocols Cell Biology Cell isolation Cell-based Assays Immunology Microbiology Microscopy |
| title | Infecting human hematopoietic stem and progenitor cells with SARS-CoV-2 |
| title_full | Infecting human hematopoietic stem and progenitor cells with SARS-CoV-2 |
| title_fullStr | Infecting human hematopoietic stem and progenitor cells with SARS-CoV-2 |
| title_full_unstemmed | Infecting human hematopoietic stem and progenitor cells with SARS-CoV-2 |
| title_short | Infecting human hematopoietic stem and progenitor cells with SARS-CoV-2 |
| title_sort | infecting human hematopoietic stem and progenitor cells with sars cov 2 |
| topic | Cell Biology Cell isolation Cell-based Assays Immunology Microbiology Microscopy |
| url | http://www.sciencedirect.com/science/article/pii/S2666166721006092 |
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