Plant growth-promoting effect and genomic analysis of the P. putida LWPZF isolated from C. japonicum rhizosphere
Abstract Plant growth-promoting rhizobacteria are a type of beneficial bacteria which inhabit in the rhizosphere and possess the abilities to promote plant growth. Pseudomonas putida LWPZF is a plant growth-promoting bacterium isolated from the rhizosphere soil of Cercidiphyllum japonicum. Inoculati...
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| Format: | Article |
| Language: | English |
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SpringerOpen
2022-08-01
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| Series: | AMB Express |
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| Online Access: | https://doi.org/10.1186/s13568-022-01445-3 |
| _version_ | 1798038062908833792 |
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| author | Tingting Jin Jiahong Ren Yunling Li Bianxia Bai Ruixiang Liu Ying Wang |
| author_facet | Tingting Jin Jiahong Ren Yunling Li Bianxia Bai Ruixiang Liu Ying Wang |
| author_sort | Tingting Jin |
| collection | DOAJ |
| description | Abstract Plant growth-promoting rhizobacteria are a type of beneficial bacteria which inhabit in the rhizosphere and possess the abilities to promote plant growth. Pseudomonas putida LWPZF is a plant growth-promoting bacterium isolated from the rhizosphere soil of Cercidiphyllum japonicum. Inoculation treatment with LWPZF could significantly promote the growth of C. japonicum seedlings. P. putida LWPZF has a variety of plant growth-promoting properties, including the ability to solubilize phosphate, synthesize ACC deaminase and IAA. The P. putida LWPZF genome contained a circular chromosome (6,259,530 bp) and a circular plasmid (160,969 bp) with G+C contents of 61.75% and 58.25%, respectively. There were 5632 and 169 predicted protein-coding sequences (CDSs) on the chromosome and the plasmid respectively. Genome sequence analysis revealed lots of genes associated with biosynthesis of IAA, pyoverdine, ACC deaminase, trehalose, volatiles acetoin and 2,3-butanediol, 4-hydroxybenzoate, as well as gluconic acid contributing phosphate solubilization. Additionally, we identified many heavy metal resistance genes, including arsenate, copper, chromate, cobalt-zinc-cadmium, and mercury. These results suggest that P. putida LWPZF shows strong potential in the fields of biofertilizer, biocontrol and heavy metal contamination soil remediation. The data presented in this study will allow us to better understand the mechanisms of plant growth promotion, biocontrol, and anti-heavy metal of P. putida LWPZF. |
| first_indexed | 2024-04-11T21:35:06Z |
| format | Article |
| id | doaj.art-c23b54c91eda478abb8f6cc4a70a0017 |
| institution | Directory Open Access Journal |
| issn | 2191-0855 |
| language | English |
| last_indexed | 2024-04-11T21:35:06Z |
| publishDate | 2022-08-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | AMB Express |
| spelling | doaj.art-c23b54c91eda478abb8f6cc4a70a00172022-12-22T04:01:47ZengSpringerOpenAMB Express2191-08552022-08-0112111410.1186/s13568-022-01445-3Plant growth-promoting effect and genomic analysis of the P. putida LWPZF isolated from C. japonicum rhizosphereTingting Jin0Jiahong Ren1Yunling Li2Bianxia Bai3Ruixiang Liu4Ying Wang5Department of Life Sciences, Changzhi UniversityDepartment of Life Sciences, Changzhi UniversityDepartment of Life Sciences, Changzhi UniversityDepartment of Life Sciences, Changzhi UniversityDepartment of Life Sciences, Changzhi UniversityDepartment of Life Sciences, Changzhi UniversityAbstract Plant growth-promoting rhizobacteria are a type of beneficial bacteria which inhabit in the rhizosphere and possess the abilities to promote plant growth. Pseudomonas putida LWPZF is a plant growth-promoting bacterium isolated from the rhizosphere soil of Cercidiphyllum japonicum. Inoculation treatment with LWPZF could significantly promote the growth of C. japonicum seedlings. P. putida LWPZF has a variety of plant growth-promoting properties, including the ability to solubilize phosphate, synthesize ACC deaminase and IAA. The P. putida LWPZF genome contained a circular chromosome (6,259,530 bp) and a circular plasmid (160,969 bp) with G+C contents of 61.75% and 58.25%, respectively. There were 5632 and 169 predicted protein-coding sequences (CDSs) on the chromosome and the plasmid respectively. Genome sequence analysis revealed lots of genes associated with biosynthesis of IAA, pyoverdine, ACC deaminase, trehalose, volatiles acetoin and 2,3-butanediol, 4-hydroxybenzoate, as well as gluconic acid contributing phosphate solubilization. Additionally, we identified many heavy metal resistance genes, including arsenate, copper, chromate, cobalt-zinc-cadmium, and mercury. These results suggest that P. putida LWPZF shows strong potential in the fields of biofertilizer, biocontrol and heavy metal contamination soil remediation. The data presented in this study will allow us to better understand the mechanisms of plant growth promotion, biocontrol, and anti-heavy metal of P. putida LWPZF.https://doi.org/10.1186/s13568-022-01445-3Plant growth promotionPseudomonas putidaGenome analysisHeavy metal resistanceAntifungal activity |
| spellingShingle | Tingting Jin Jiahong Ren Yunling Li Bianxia Bai Ruixiang Liu Ying Wang Plant growth-promoting effect and genomic analysis of the P. putida LWPZF isolated from C. japonicum rhizosphere AMB Express Plant growth promotion Pseudomonas putida Genome analysis Heavy metal resistance Antifungal activity |
| title | Plant growth-promoting effect and genomic analysis of the P. putida LWPZF isolated from C. japonicum rhizosphere |
| title_full | Plant growth-promoting effect and genomic analysis of the P. putida LWPZF isolated from C. japonicum rhizosphere |
| title_fullStr | Plant growth-promoting effect and genomic analysis of the P. putida LWPZF isolated from C. japonicum rhizosphere |
| title_full_unstemmed | Plant growth-promoting effect and genomic analysis of the P. putida LWPZF isolated from C. japonicum rhizosphere |
| title_short | Plant growth-promoting effect and genomic analysis of the P. putida LWPZF isolated from C. japonicum rhizosphere |
| title_sort | plant growth promoting effect and genomic analysis of the p putida lwpzf isolated from c japonicum rhizosphere |
| topic | Plant growth promotion Pseudomonas putida Genome analysis Heavy metal resistance Antifungal activity |
| url | https://doi.org/10.1186/s13568-022-01445-3 |
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