LC-MS/MS Screening, Total Phenolic, Flavonoid and Antioxidant Contents of Crude Extracts from Three Asclepiadaceae Species Growing in Jordan
This study aimed to evaluate the antioxidant activity and total phenolic content (TPC) and total flavonoid content (TFC) of crude extracts obtained from three <i>Asclepiadaceae</i> species, namely, <i>Calotropis procera</i> L., <i>Peruglaria tomentosa</i> L., and...
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MDPI AG
2022-01-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/3/859 |
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| author | Yousef Al-Dalahmeh Nezar Al-Bataineh Sara S. Al-Balawi Jamil N. Lahham Idrees F. Al-Momani Mohammed S. Al-Sheraideh Abdulraouf S. Mayyas Sultan T. Abu Orabi Mahmoud A. Al-Qudah |
| author_facet | Yousef Al-Dalahmeh Nezar Al-Bataineh Sara S. Al-Balawi Jamil N. Lahham Idrees F. Al-Momani Mohammed S. Al-Sheraideh Abdulraouf S. Mayyas Sultan T. Abu Orabi Mahmoud A. Al-Qudah |
| author_sort | Yousef Al-Dalahmeh |
| collection | DOAJ |
| description | This study aimed to evaluate the antioxidant activity and total phenolic content (TPC) and total flavonoid content (TFC) of crude extracts obtained from three <i>Asclepiadaceae</i> species, namely, <i>Calotropis procera</i> L., <i>Peruglaria tomentosa</i> L., and <i>Pentatropis spiralis</i> (Forsk.) Decne. Both butanol and aq. methanol extracts of the three species showed the highest amount of phenol and flavonoid contents, which exhibited the greatest antioxidant activity in the scavenging of 2,2-diphenyl-2-picrylhydrazyl free radical (DPPH), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt radical cation (ABTS), ferrous chelating effect (FIC), and hydroxyl radical (HDR) assays. Phytochemical screening of the extracts revealed the presence of alkaloids, tannins, sponins, flavonoids, terpenoids, and glycosides. LC-MS analysis was carried out to identify the major compounds from each crude extract. A total of 12 phenolic compounds in the extracts of the 3 species were identified and quantified, including 9 flavonoids, 2 hydroxybenzoic acids, and 3 hydroxycinnamic acids. The current study also revealed a good correlation between total phenolic contents and the observed antioxidant activity of the crude extracts. |
| first_indexed | 2024-03-09T23:28:38Z |
| format | Article |
| id | doaj.art-c23c5e63ed814dcfba45dcae02cdd14b |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T23:28:38Z |
| publishDate | 2022-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-c23c5e63ed814dcfba45dcae02cdd14b2023-11-23T17:13:35ZengMDPI AGMolecules1420-30492022-01-0127385910.3390/molecules27030859LC-MS/MS Screening, Total Phenolic, Flavonoid and Antioxidant Contents of Crude Extracts from Three Asclepiadaceae Species Growing in JordanYousef Al-Dalahmeh0Nezar Al-Bataineh1Sara S. Al-Balawi2Jamil N. Lahham3Idrees F. Al-Momani4Mohammed S. Al-Sheraideh5Abdulraouf S. Mayyas6Sultan T. Abu Orabi7Mahmoud A. Al-Qudah8Department of Applied Pharmaceutical Sciences, Faculty of Pharmacy, Isra University, Amman 11622, JordanCollege of Pharmacy, Al Ain University of Science and Technology, Abu Dhabi P.O. Box 6414, United Arab EmiratesDepartment of Chemistry, Faculty of Science, Yarmouk University, P.O. Box 566, Irbid 21163, JordanDepartment of Biological Sciences, Faculty of Science, Yarmouk University, Irbid 21163, JordanDepartment of Chemistry, Faculty of Science, Yarmouk University, P.O. Box 566, Irbid 21163, JordanChemistry Department, College of Science, Imam Abdulrahman Bin Faisal University, P.O. Box 383, Dammam 31113, Saudi ArabiaDepartment of Conservation Science, Queen Rania Faculty of Tourism and Heritage, The Hashemite University, Zarqa 13133, JordanDepartment of Chemistry, Faculty of Science, Yarmouk University, P.O. Box 566, Irbid 21163, JordanDepartment of Chemistry, Faculty of Science, Yarmouk University, P.O. Box 566, Irbid 21163, JordanThis study aimed to evaluate the antioxidant activity and total phenolic content (TPC) and total flavonoid content (TFC) of crude extracts obtained from three <i>Asclepiadaceae</i> species, namely, <i>Calotropis procera</i> L., <i>Peruglaria tomentosa</i> L., and <i>Pentatropis spiralis</i> (Forsk.) Decne. Both butanol and aq. methanol extracts of the three species showed the highest amount of phenol and flavonoid contents, which exhibited the greatest antioxidant activity in the scavenging of 2,2-diphenyl-2-picrylhydrazyl free radical (DPPH), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt radical cation (ABTS), ferrous chelating effect (FIC), and hydroxyl radical (HDR) assays. Phytochemical screening of the extracts revealed the presence of alkaloids, tannins, sponins, flavonoids, terpenoids, and glycosides. LC-MS analysis was carried out to identify the major compounds from each crude extract. A total of 12 phenolic compounds in the extracts of the 3 species were identified and quantified, including 9 flavonoids, 2 hydroxybenzoic acids, and 3 hydroxycinnamic acids. The current study also revealed a good correlation between total phenolic contents and the observed antioxidant activity of the crude extracts.https://www.mdpi.com/1420-3049/27/3/859total phenolicflavonoidantioxidant properties<i>Calotropis procera</i><i>Peruglaria tomentosa</i><i>Pentatropis spiralis</i> |
| spellingShingle | Yousef Al-Dalahmeh Nezar Al-Bataineh Sara S. Al-Balawi Jamil N. Lahham Idrees F. Al-Momani Mohammed S. Al-Sheraideh Abdulraouf S. Mayyas Sultan T. Abu Orabi Mahmoud A. Al-Qudah LC-MS/MS Screening, Total Phenolic, Flavonoid and Antioxidant Contents of Crude Extracts from Three Asclepiadaceae Species Growing in Jordan Molecules total phenolic flavonoid antioxidant properties <i>Calotropis procera</i> <i>Peruglaria tomentosa</i> <i>Pentatropis spiralis</i> |
| title | LC-MS/MS Screening, Total Phenolic, Flavonoid and Antioxidant Contents of Crude Extracts from Three Asclepiadaceae Species Growing in Jordan |
| title_full | LC-MS/MS Screening, Total Phenolic, Flavonoid and Antioxidant Contents of Crude Extracts from Three Asclepiadaceae Species Growing in Jordan |
| title_fullStr | LC-MS/MS Screening, Total Phenolic, Flavonoid and Antioxidant Contents of Crude Extracts from Three Asclepiadaceae Species Growing in Jordan |
| title_full_unstemmed | LC-MS/MS Screening, Total Phenolic, Flavonoid and Antioxidant Contents of Crude Extracts from Three Asclepiadaceae Species Growing in Jordan |
| title_short | LC-MS/MS Screening, Total Phenolic, Flavonoid and Antioxidant Contents of Crude Extracts from Three Asclepiadaceae Species Growing in Jordan |
| title_sort | lc ms ms screening total phenolic flavonoid and antioxidant contents of crude extracts from three asclepiadaceae species growing in jordan |
| topic | total phenolic flavonoid antioxidant properties <i>Calotropis procera</i> <i>Peruglaria tomentosa</i> <i>Pentatropis spiralis</i> |
| url | https://www.mdpi.com/1420-3049/27/3/859 |
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