Anti-Steatotic Effects of <i>Chlorella vulgaris</i>, <i>Nannochloropsis gaditana</i> and <i>Gracilaria vermiculophylla</i> Algae Extracts in AML-12 Hepatocytes
Non-alcoholic fatty liver disease (NAFLD) is considered the most common chronic liver alteration whose prevalence is increasing in Western countries. Microalgae and macroalgae have attracted great interest due to the high content in bioactive compounds with beneficial effects on health. The aim of t...
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MDPI AG
2023-04-01
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| Series: | Nutrients |
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| Online Access: | https://www.mdpi.com/2072-6643/15/8/1960 |
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| author | Maitane González-Arceo Jenifer Trepiana Leixuri Aguirre Jone Ibarruri Marta Martínez-Sanz Marta Cebrián Isidra Recio María P. Portillo Saioa Gómez-Zorita |
| author_facet | Maitane González-Arceo Jenifer Trepiana Leixuri Aguirre Jone Ibarruri Marta Martínez-Sanz Marta Cebrián Isidra Recio María P. Portillo Saioa Gómez-Zorita |
| author_sort | Maitane González-Arceo |
| collection | DOAJ |
| description | Non-alcoholic fatty liver disease (NAFLD) is considered the most common chronic liver alteration whose prevalence is increasing in Western countries. Microalgae and macroalgae have attracted great interest due to the high content in bioactive compounds with beneficial effects on health. The aim of the present study is to assess the potential interest of extracts rich in proteins obtained from the microalgae <i>Chlorella vulgaris</i> and <i>Nannochloropsis gaditana</i> and the macroalga <i>Gracilaria vermiculophylla</i> in the prevention of lipid accumulation in AML-12 hepatocytes. Toxicity was not observed at any of the tested doses. Both microalgae and the macroalga were effective in preventing triglyceride accumulation, with <i>Nannochloropsis gaditana</i> being the most effective one. Although the three algae extracts were able to increase different catabolic pathways involved in triglyceride metabolism, the mechanisms underlying the anti-steatotic effect were different in each algae extract. In conclusion, the present study demonstrates that <i>Chlorella vulgaris</i>, <i>Nannochloropsis gaditana</i> and <i>Gracilaria vermiculophylla</i> extracts are able to partially prevent the accumulation of triglycerides induced by palmitic acid in cultured hepatocytes, a model used to mimic the steatosis induced in liver by dietary patterns rich in saturated fat. |
| first_indexed | 2024-03-11T04:39:39Z |
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| id | doaj.art-c23cd4f7686343e689d01d1de4a49e69 |
| institution | Directory Open Access Journal |
| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-03-11T04:39:39Z |
| publishDate | 2023-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nutrients |
| spelling | doaj.art-c23cd4f7686343e689d01d1de4a49e692023-11-17T20:46:55ZengMDPI AGNutrients2072-66432023-04-01158196010.3390/nu15081960Anti-Steatotic Effects of <i>Chlorella vulgaris</i>, <i>Nannochloropsis gaditana</i> and <i>Gracilaria vermiculophylla</i> Algae Extracts in AML-12 HepatocytesMaitane González-Arceo0Jenifer Trepiana1Leixuri Aguirre2Jone Ibarruri3Marta Martínez-Sanz4Marta Cebrián5Isidra Recio6María P. Portillo7Saioa Gómez-Zorita8Nutrition and Obesity Group, Department of Nutrition and Food Science, University of the Basque Country (UPV/EHU) and Lucio Lascaray Research Institute, 01006 Vitoria-Gasteiz, SpainNutrition and Obesity Group, Department of Nutrition and Food Science, University of the Basque Country (UPV/EHU) and Lucio Lascaray Research Institute, 01006 Vitoria-Gasteiz, SpainNutrition and Obesity Group, Department of Nutrition and Food Science, University of the Basque Country (UPV/EHU) and Lucio Lascaray Research Institute, 01006 Vitoria-Gasteiz, SpainAZTI, Food Research, Basque Research and Technology Alliance (BRTA), Parque Tecnológico de Bizkaia, Astondo Bidea, 609, 48160 Derio, SpainInstituto de Investigación en Ciencias de la Alimentación, CIAL (CSIC-UAM), Nicolás Cabrera, 9, 28049 Madrid, SpainAZTI, Food Research, Basque Research and Technology Alliance (BRTA), Parque Tecnológico de Bizkaia, Astondo Bidea, 609, 48160 Derio, SpainInstituto de Investigación en Ciencias de la Alimentación, CIAL (CSIC-UAM), Nicolás Cabrera, 9, 28049 Madrid, SpainNutrition and Obesity Group, Department of Nutrition and Food Science, University of the Basque Country (UPV/EHU) and Lucio Lascaray Research Institute, 01006 Vitoria-Gasteiz, SpainNutrition and Obesity Group, Department of Nutrition and Food Science, University of the Basque Country (UPV/EHU) and Lucio Lascaray Research Institute, 01006 Vitoria-Gasteiz, SpainNon-alcoholic fatty liver disease (NAFLD) is considered the most common chronic liver alteration whose prevalence is increasing in Western countries. Microalgae and macroalgae have attracted great interest due to the high content in bioactive compounds with beneficial effects on health. The aim of the present study is to assess the potential interest of extracts rich in proteins obtained from the microalgae <i>Chlorella vulgaris</i> and <i>Nannochloropsis gaditana</i> and the macroalga <i>Gracilaria vermiculophylla</i> in the prevention of lipid accumulation in AML-12 hepatocytes. Toxicity was not observed at any of the tested doses. Both microalgae and the macroalga were effective in preventing triglyceride accumulation, with <i>Nannochloropsis gaditana</i> being the most effective one. Although the three algae extracts were able to increase different catabolic pathways involved in triglyceride metabolism, the mechanisms underlying the anti-steatotic effect were different in each algae extract. In conclusion, the present study demonstrates that <i>Chlorella vulgaris</i>, <i>Nannochloropsis gaditana</i> and <i>Gracilaria vermiculophylla</i> extracts are able to partially prevent the accumulation of triglycerides induced by palmitic acid in cultured hepatocytes, a model used to mimic the steatosis induced in liver by dietary patterns rich in saturated fat.https://www.mdpi.com/2072-6643/15/8/1960microalgaemacroalgaeseaweedextractsnon-alcoholic fatty liver diseasesteatosis |
| spellingShingle | Maitane González-Arceo Jenifer Trepiana Leixuri Aguirre Jone Ibarruri Marta Martínez-Sanz Marta Cebrián Isidra Recio María P. Portillo Saioa Gómez-Zorita Anti-Steatotic Effects of <i>Chlorella vulgaris</i>, <i>Nannochloropsis gaditana</i> and <i>Gracilaria vermiculophylla</i> Algae Extracts in AML-12 Hepatocytes Nutrients microalgae macroalgae seaweed extracts non-alcoholic fatty liver disease steatosis |
| title | Anti-Steatotic Effects of <i>Chlorella vulgaris</i>, <i>Nannochloropsis gaditana</i> and <i>Gracilaria vermiculophylla</i> Algae Extracts in AML-12 Hepatocytes |
| title_full | Anti-Steatotic Effects of <i>Chlorella vulgaris</i>, <i>Nannochloropsis gaditana</i> and <i>Gracilaria vermiculophylla</i> Algae Extracts in AML-12 Hepatocytes |
| title_fullStr | Anti-Steatotic Effects of <i>Chlorella vulgaris</i>, <i>Nannochloropsis gaditana</i> and <i>Gracilaria vermiculophylla</i> Algae Extracts in AML-12 Hepatocytes |
| title_full_unstemmed | Anti-Steatotic Effects of <i>Chlorella vulgaris</i>, <i>Nannochloropsis gaditana</i> and <i>Gracilaria vermiculophylla</i> Algae Extracts in AML-12 Hepatocytes |
| title_short | Anti-Steatotic Effects of <i>Chlorella vulgaris</i>, <i>Nannochloropsis gaditana</i> and <i>Gracilaria vermiculophylla</i> Algae Extracts in AML-12 Hepatocytes |
| title_sort | anti steatotic effects of i chlorella vulgaris i i nannochloropsis gaditana i and i gracilaria vermiculophylla i algae extracts in aml 12 hepatocytes |
| topic | microalgae macroalgae seaweed extracts non-alcoholic fatty liver disease steatosis |
| url | https://www.mdpi.com/2072-6643/15/8/1960 |
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