Environmentally-induced epigenetic conversion of a piRNA cluster
Transposable element (TE) activity is repressed in animal gonads by PIWI-interacting RNAs (piRNAs) produced by piRNA clusters. Current models in flies propose that germinal piRNA clusters are functionally defined by the maternal inheritance of piRNAs produced during the previous generation. Taking a...
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2019-03-01
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| Series: | eLife |
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| Online Access: | https://elifesciences.org/articles/39842 |
| _version_ | 1811253271116906496 |
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| author | Karine Casier Valérie Delmarre Nathalie Gueguen Catherine Hermant Elise Viodé Chantal Vaury Stéphane Ronsseray Emilie Brasset Laure Teysset Antoine Boivin |
| author_facet | Karine Casier Valérie Delmarre Nathalie Gueguen Catherine Hermant Elise Viodé Chantal Vaury Stéphane Ronsseray Emilie Brasset Laure Teysset Antoine Boivin |
| author_sort | Karine Casier |
| collection | DOAJ |
| description | Transposable element (TE) activity is repressed in animal gonads by PIWI-interacting RNAs (piRNAs) produced by piRNA clusters. Current models in flies propose that germinal piRNA clusters are functionally defined by the maternal inheritance of piRNAs produced during the previous generation. Taking advantage of an inactive, but ready to go, cluster of P-element derived transgene insertions in Drosophila melanogaster, we show here that raising flies at high temperature (29°C) instead of 25°C triggers the stable conversion of this locus from inactive into actively producing functional piRNAs. The increase of antisense transcripts from the cluster at 29°C combined with the requirement of transcription of euchromatic homologous sequences, suggests a role of double stranded RNA in the production of de novo piRNAs. This report describes the first case of the establishment of an active piRNA cluster by environmental changes in the absence of maternal inheritance of homologous piRNAs.Editorial note: This article has been through an editorial process in which the authors decide how to respond to the issues raised during peer review. The Reviewing Editor's assessment is that all the issues have been addressed (see decision letter). |
| first_indexed | 2024-04-12T16:48:25Z |
| format | Article |
| id | doaj.art-c2425231e6fa46a2afbb55f55502ae50 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-12T16:48:25Z |
| publishDate | 2019-03-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-c2425231e6fa46a2afbb55f55502ae502022-12-22T03:24:30ZengeLife Sciences Publications LtdeLife2050-084X2019-03-01810.7554/eLife.39842Environmentally-induced epigenetic conversion of a piRNA clusterKarine Casier0https://orcid.org/0000-0001-6825-8057Valérie Delmarre1Nathalie Gueguen2Catherine Hermant3Elise Viodé4https://orcid.org/0000-0002-5058-2710Chantal Vaury5Stéphane Ronsseray6Emilie Brasset7Laure Teysset8https://orcid.org/0000-0002-7413-1850Antoine Boivin9https://orcid.org/0000-0001-8671-1599Laboratoire Biologie du Développement, UMR7622, Sorbonne Université, CNRS, Institut de Biologie Paris-Seine, Paris, FranceLaboratoire Biologie du Développement, UMR7622, Sorbonne Université, CNRS, Institut de Biologie Paris-Seine, Paris, FranceGReD, Université Clermont Auvergne, CNRS, INSERM, BP 10448, Clermont-Ferrand, FranceLaboratoire Biologie du Développement, UMR7622, Sorbonne Université, CNRS, Institut de Biologie Paris-Seine, Paris, FranceLaboratoire Biologie du Développement, UMR7622, Sorbonne Université, CNRS, Institut de Biologie Paris-Seine, Paris, FranceGReD, Université Clermont Auvergne, CNRS, INSERM, BP 10448, Clermont-Ferrand, FranceLaboratoire Biologie du Développement, UMR7622, Sorbonne Université, CNRS, Institut de Biologie Paris-Seine, Paris, FranceGReD, Université Clermont Auvergne, CNRS, INSERM, BP 10448, Clermont-Ferrand, FranceLaboratoire Biologie du Développement, UMR7622, Sorbonne Université, CNRS, Institut de Biologie Paris-Seine, Paris, FranceLaboratoire Biologie du Développement, UMR7622, Sorbonne Université, CNRS, Institut de Biologie Paris-Seine, Paris, FranceTransposable element (TE) activity is repressed in animal gonads by PIWI-interacting RNAs (piRNAs) produced by piRNA clusters. Current models in flies propose that germinal piRNA clusters are functionally defined by the maternal inheritance of piRNAs produced during the previous generation. Taking advantage of an inactive, but ready to go, cluster of P-element derived transgene insertions in Drosophila melanogaster, we show here that raising flies at high temperature (29°C) instead of 25°C triggers the stable conversion of this locus from inactive into actively producing functional piRNAs. The increase of antisense transcripts from the cluster at 29°C combined with the requirement of transcription of euchromatic homologous sequences, suggests a role of double stranded RNA in the production of de novo piRNAs. This report describes the first case of the establishment of an active piRNA cluster by environmental changes in the absence of maternal inheritance of homologous piRNAs.Editorial note: This article has been through an editorial process in which the authors decide how to respond to the issues raised during peer review. The Reviewing Editor's assessment is that all the issues have been addressed (see decision letter).https://elifesciences.org/articles/39842epigeneticsgermlinenon coding small RNAtransposable elementtemperatureheterochromatin |
| spellingShingle | Karine Casier Valérie Delmarre Nathalie Gueguen Catherine Hermant Elise Viodé Chantal Vaury Stéphane Ronsseray Emilie Brasset Laure Teysset Antoine Boivin Environmentally-induced epigenetic conversion of a piRNA cluster eLife epigenetics germline non coding small RNA transposable element temperature heterochromatin |
| title | Environmentally-induced epigenetic conversion of a piRNA cluster |
| title_full | Environmentally-induced epigenetic conversion of a piRNA cluster |
| title_fullStr | Environmentally-induced epigenetic conversion of a piRNA cluster |
| title_full_unstemmed | Environmentally-induced epigenetic conversion of a piRNA cluster |
| title_short | Environmentally-induced epigenetic conversion of a piRNA cluster |
| title_sort | environmentally induced epigenetic conversion of a pirna cluster |
| topic | epigenetics germline non coding small RNA transposable element temperature heterochromatin |
| url | https://elifesciences.org/articles/39842 |
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