Heat Stress Resistance Mechanisms of Two Cucumber Varieties from Different Regions
High temperatures affect the yield and quality of vegetable crops. Unlike thermosensitive plants, thermotolerant plants have excellent systems for withstanding heat stress. This study evaluated various heat resistance indexes of the thermotolerant cucumber (TT) and thermosensitive cucumber (TS) plan...
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MDPI AG
2022-02-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/3/1817 |
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| author | Bingwei Yu Fangyan Ming Yonggui Liang Yixi Wang Yuwei Gan Zhengkun Qiu Shuangshuang Yan Bihao Cao |
| author_facet | Bingwei Yu Fangyan Ming Yonggui Liang Yixi Wang Yuwei Gan Zhengkun Qiu Shuangshuang Yan Bihao Cao |
| author_sort | Bingwei Yu |
| collection | DOAJ |
| description | High temperatures affect the yield and quality of vegetable crops. Unlike thermosensitive plants, thermotolerant plants have excellent systems for withstanding heat stress. This study evaluated various heat resistance indexes of the thermotolerant cucumber (TT) and thermosensitive cucumber (TS) plants at the seedling stage. The similarities and differences between the regulatory genes were assessed through transcriptome analysis to understand the mechanisms for heat stress resistance in cucumber. The TT plants exhibited enhanced leaf status, photosystem, root viability, and ROS scavenging under high temperature compared to the TS plants. Additionally, transcriptome analysis showed that the genes involved in photosynthesis, the chlorophyll metabolism, and defense responses were upregulated in TT plants but downregulated in TS plants. Zeatin riboside (ZR), brassinosteroid (BR), and jasmonic acid (JA) levels were higher in TT plants than in TS. The heat stress increased gibberellic acid (GA) and indoleacetic acid (IAA) levels in both plant lines; however, the level of GA was higher in TT. Correlation and interaction analyses revealed that heat cucumber heat resistance is regulated by a few transcription factor family genes and metabolic pathways. Our study revealed different phenotypic and physiological mechanisms of the heat response by the thermotolerant and thermosensitive cucumber plants. The plants were also shown to exhibit different expression profiles and metabolic pathways. The heat resistant pathways and genes of two cucumber varieties were also identified. These results enhance our understanding of the molecular mechanisms of cucumber response to high-temperature stress. |
| first_indexed | 2024-03-09T23:44:09Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T23:44:09Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-c245776ef64740d8a74e944c8949f3662023-11-23T16:47:05ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-02-01233181710.3390/ijms23031817Heat Stress Resistance Mechanisms of Two Cucumber Varieties from Different RegionsBingwei Yu0Fangyan Ming1Yonggui Liang2Yixi Wang3Yuwei Gan4Zhengkun Qiu5Shuangshuang Yan6Bihao Cao7Key Laboratory of Horticultural Crop Biology and Germplasm Innovation in South China, Ministry of Agriculture, College of Horticulture, South China Agricultural University, Guangzhou 510642, ChinaKey Laboratory of Horticultural Crop Biology and Germplasm Innovation in South China, Ministry of Agriculture, College of Horticulture, South China Agricultural University, Guangzhou 510642, ChinaKey Laboratory of Horticultural Crop Biology and Germplasm Innovation in South China, Ministry of Agriculture, College of Horticulture, South China Agricultural University, Guangzhou 510642, ChinaKey Laboratory of Horticultural Crop Biology and Germplasm Innovation in South China, Ministry of Agriculture, College of Horticulture, South China Agricultural University, Guangzhou 510642, ChinaKey Laboratory of Horticultural Crop Biology and Germplasm Innovation in South China, Ministry of Agriculture, College of Horticulture, South China Agricultural University, Guangzhou 510642, ChinaKey Laboratory of Horticultural Crop Biology and Germplasm Innovation in South China, Ministry of Agriculture, College of Horticulture, South China Agricultural University, Guangzhou 510642, ChinaKey Laboratory of Horticultural Crop Biology and Germplasm Innovation in South China, Ministry of Agriculture, College of Horticulture, South China Agricultural University, Guangzhou 510642, ChinaKey Laboratory of Horticultural Crop Biology and Germplasm Innovation in South China, Ministry of Agriculture, College of Horticulture, South China Agricultural University, Guangzhou 510642, ChinaHigh temperatures affect the yield and quality of vegetable crops. Unlike thermosensitive plants, thermotolerant plants have excellent systems for withstanding heat stress. This study evaluated various heat resistance indexes of the thermotolerant cucumber (TT) and thermosensitive cucumber (TS) plants at the seedling stage. The similarities and differences between the regulatory genes were assessed through transcriptome analysis to understand the mechanisms for heat stress resistance in cucumber. The TT plants exhibited enhanced leaf status, photosystem, root viability, and ROS scavenging under high temperature compared to the TS plants. Additionally, transcriptome analysis showed that the genes involved in photosynthesis, the chlorophyll metabolism, and defense responses were upregulated in TT plants but downregulated in TS plants. Zeatin riboside (ZR), brassinosteroid (BR), and jasmonic acid (JA) levels were higher in TT plants than in TS. The heat stress increased gibberellic acid (GA) and indoleacetic acid (IAA) levels in both plant lines; however, the level of GA was higher in TT. Correlation and interaction analyses revealed that heat cucumber heat resistance is regulated by a few transcription factor family genes and metabolic pathways. Our study revealed different phenotypic and physiological mechanisms of the heat response by the thermotolerant and thermosensitive cucumber plants. The plants were also shown to exhibit different expression profiles and metabolic pathways. The heat resistant pathways and genes of two cucumber varieties were also identified. These results enhance our understanding of the molecular mechanisms of cucumber response to high-temperature stress.https://www.mdpi.com/1422-0067/23/3/1817heat stressthermotolerancetranscriptomehormonetranscription factors |
| spellingShingle | Bingwei Yu Fangyan Ming Yonggui Liang Yixi Wang Yuwei Gan Zhengkun Qiu Shuangshuang Yan Bihao Cao Heat Stress Resistance Mechanisms of Two Cucumber Varieties from Different Regions International Journal of Molecular Sciences heat stress thermotolerance transcriptome hormone transcription factors |
| title | Heat Stress Resistance Mechanisms of Two Cucumber Varieties from Different Regions |
| title_full | Heat Stress Resistance Mechanisms of Two Cucumber Varieties from Different Regions |
| title_fullStr | Heat Stress Resistance Mechanisms of Two Cucumber Varieties from Different Regions |
| title_full_unstemmed | Heat Stress Resistance Mechanisms of Two Cucumber Varieties from Different Regions |
| title_short | Heat Stress Resistance Mechanisms of Two Cucumber Varieties from Different Regions |
| title_sort | heat stress resistance mechanisms of two cucumber varieties from different regions |
| topic | heat stress thermotolerance transcriptome hormone transcription factors |
| url | https://www.mdpi.com/1422-0067/23/3/1817 |
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