Peptides of Trypsin Hydrolyzate in Bovine Colostrum
Bovine colostrum contains biologically active substances, e.g., immunoglobulins, peptides, and cytokines, which makes it a logical component of numerous functional products. Colostrum peptides also possess antimicrobial activity. This bioavailability increases during colostrum fermentation with prot...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | Russian |
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Kemerovo State University
2023-03-01
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| Series: | Техника и технология пищевых производств |
| Subjects: | |
| Online Access: | https://fptt.ru/en/issues/21374/21415/ |
| _version_ | 1797850996165050368 |
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| author | Sergei L. Tikhonov Natalia V. Tikhonova Khatam Kh. Tursunov Irina G. Danilova Vladimir A. Lazarev |
| author_facet | Sergei L. Tikhonov Natalia V. Tikhonova Khatam Kh. Tursunov Irina G. Danilova Vladimir A. Lazarev |
| author_sort | Sergei L. Tikhonov |
| collection | DOAJ |
| description | Bovine colostrum contains biologically active substances, e.g., immunoglobulins, peptides, and cytokines, which makes it a logical component of numerous functional products. Colostrum peptides also possess antimicrobial activity. This bioavailability increases during colostrum fermentation with proteolytic enzymes. The research objective was to describe peptides isolated from the trypsic hydrolyzate supernatant of bovine colostrum and to evaluate their antimicrobial and antifungal properties.
The supernatant of trypsin hydrolyzate of bovine colostrum was isolated by centrifugation at 3900 rpm for 7 min. The supernatant was separated by preparative chromatography. Its peptide composition was determined on a MALDI-TOF mass spectrometer, while the protein sequences were deciphered using the Mascot database. Proteins were precipitated with ammonium sulfate, and the antimicrobial activity was measured by the disk-diffusion method against gram-positive and gram-negative bacteria and dipoloid fungi. Strains were cultivated on a thick LB nutrient medium at 37°C. The antimicrobial activity was defined experimentally on Wistar rats infected intraperitoneally with Salmonella enteritidis 92.
The trypsin hydrolyzate supernatant of bovine colostrum revealed four peptides, one of which belonged to short peptides, while the remaining three belonged to polypeptides. The isolated peptides had different molecular weights of 8.4, 6.5, 13.0, and 18 kDa. The enzymatic hydrolyzate proved bactericidal against Escherichia coli and Bacillus subtilis and demonstrated antifungal activity against Candida albicans. When rats infected with S. enteritidis 92 were administered with trypsin hydrolysate, it promoted their survival, decreased LD50, and increased the mean day of death period from 2 to 4 days.
The research proved the antimicrobial effect of colostrum peptides and suggested their immunotropic properties. The peptides obtained from the trypsin hydrolyzate supernatant of bovine colostrum can be recommended for functional food industry as part of antimicrobial products. |
| first_indexed | 2024-04-09T19:09:34Z |
| format | Article |
| id | doaj.art-c2474f2ce3e740cf9b76809c17a807a4 |
| institution | Directory Open Access Journal |
| issn | 2074-9414 2313-1748 |
| language | Russian |
| last_indexed | 2024-04-09T19:09:34Z |
| publishDate | 2023-03-01 |
| publisher | Kemerovo State University |
| record_format | Article |
| series | Техника и технология пищевых производств |
| spelling | doaj.art-c2474f2ce3e740cf9b76809c17a807a42023-04-07T04:01:07ZrusKemerovo State UniversityТехника и технология пищевых производств2074-94142313-17482023-03-0153115015810.21603/2074-9414-2023-1-2422Peptides of Trypsin Hydrolyzate in Bovine ColostrumSergei L. Tikhonov0https://orcid.org/0000-0003-4863-9834Natalia V. Tikhonova1https://orcid.org/0000-0001-5841-1791Khatam Kh. Tursunov2Irina G. Danilova3https://orcid.org/0000-0001-6841-1197Vladimir A. Lazarev4https://orcid.org/0000-0002-0470-7324Ural State University of Economics, Yekaterinburg, RussiaUral State University of Economics, Yekaterinburg, RussiaAndijan State Medical Institute, Andijan, UzbekistanInstitute of Immunology and Physiology of the Ural Branch of the Russian Academy of Sciences, Yekaterinburg, RussiaUral State University of Economics, Yekaterinburg, RussiaBovine colostrum contains biologically active substances, e.g., immunoglobulins, peptides, and cytokines, which makes it a logical component of numerous functional products. Colostrum peptides also possess antimicrobial activity. This bioavailability increases during colostrum fermentation with proteolytic enzymes. The research objective was to describe peptides isolated from the trypsic hydrolyzate supernatant of bovine colostrum and to evaluate their antimicrobial and antifungal properties. The supernatant of trypsin hydrolyzate of bovine colostrum was isolated by centrifugation at 3900 rpm for 7 min. The supernatant was separated by preparative chromatography. Its peptide composition was determined on a MALDI-TOF mass spectrometer, while the protein sequences were deciphered using the Mascot database. Proteins were precipitated with ammonium sulfate, and the antimicrobial activity was measured by the disk-diffusion method against gram-positive and gram-negative bacteria and dipoloid fungi. Strains were cultivated on a thick LB nutrient medium at 37°C. The antimicrobial activity was defined experimentally on Wistar rats infected intraperitoneally with Salmonella enteritidis 92. The trypsin hydrolyzate supernatant of bovine colostrum revealed four peptides, one of which belonged to short peptides, while the remaining three belonged to polypeptides. The isolated peptides had different molecular weights of 8.4, 6.5, 13.0, and 18 kDa. The enzymatic hydrolyzate proved bactericidal against Escherichia coli and Bacillus subtilis and demonstrated antifungal activity against Candida albicans. When rats infected with S. enteritidis 92 were administered with trypsin hydrolysate, it promoted their survival, decreased LD50, and increased the mean day of death period from 2 to 4 days. The research proved the antimicrobial effect of colostrum peptides and suggested their immunotropic properties. The peptides obtained from the trypsin hydrolyzate supernatant of bovine colostrum can be recommended for functional food industry as part of antimicrobial products.https://fptt.ru/en/issues/21374/21415/colostrummilk proteinenzymehydrolysisantimicrobial activityantifungal activitybiologically active substances |
| spellingShingle | Sergei L. Tikhonov Natalia V. Tikhonova Khatam Kh. Tursunov Irina G. Danilova Vladimir A. Lazarev Peptides of Trypsin Hydrolyzate in Bovine Colostrum Техника и технология пищевых производств colostrum milk protein enzyme hydrolysis antimicrobial activity antifungal activity biologically active substances |
| title | Peptides of Trypsin Hydrolyzate in Bovine Colostrum |
| title_full | Peptides of Trypsin Hydrolyzate in Bovine Colostrum |
| title_fullStr | Peptides of Trypsin Hydrolyzate in Bovine Colostrum |
| title_full_unstemmed | Peptides of Trypsin Hydrolyzate in Bovine Colostrum |
| title_short | Peptides of Trypsin Hydrolyzate in Bovine Colostrum |
| title_sort | peptides of trypsin hydrolyzate in bovine colostrum |
| topic | colostrum milk protein enzyme hydrolysis antimicrobial activity antifungal activity biologically active substances |
| url | https://fptt.ru/en/issues/21374/21415/ |
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