Binding pocket stabilization by high-throughput screening of yeast display libraries

Binding pocket stabilization by high-throughput screening of yeast display libraries

Protein dynamics have a great influence on the binding pockets of some therapeutic targets. Flexible protein binding sites can result in transient binding pocket formation which might have a negative impact on drug screening efforts. Here, we describe a protein engineering strategy with FK506-bindin...

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Bibliographic Details
Main Authors: Jorge A. Lerma Romero, Christian Meyners, Andreas Christmann, Lisa M. Reinbold, Anna Charalampidou, Felix Hausch, Harald Kolmar
Format: Article
Language:English
Published: Frontiers Media S.A. 2022-11-01
Series:Frontiers in Molecular Biosciences
Subjects:
protein engineering
transient binding pocket
yeast display
flow cytometry
FKBP
high-throughput screening
Online Access:https://www.frontiersin.org/articles/10.3389/fmolb.2022.1023131/full

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