Antimicrobial resistance profiles of human Brucella melitensis isolates in three different microdilution broths: the first multicentre study in Bosnia and Herzegovina
ABSTRACT: Objectives: Brucellosis is a ubiquitous emergent bacterial zoonotic disease causing significant human morbidity in Bosnia and Herzegovina. So far, a high rate of resistant Brucella has been found worldwide. This study prospectively analysed the rates of resistance among human Brucella mel...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-06-01
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| Series: | Journal of Global Antimicrobial Resistance |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213716522000406 |
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| author | Jurica Arapović Gordan Kompes Kanita Dedić Sanela Teskeredžić Maja Ostojić Maja Travar Nijaz Tihić Jasminka Delić Siniša Skočibušić Minela Zekiri-Sivro Antonija Verhaz Dilista Piljić Luka Laura Sanja Duvnjak Maja Zdelar-Tuk Maja Arapović Enisa Šabotić Irena Reil Jadranka Nikolić Sead Ahmetagić Željko Cvetnić Boris Habrun Mile Bosilkovski Silvio Špičić |
| author_facet | Jurica Arapović Gordan Kompes Kanita Dedić Sanela Teskeredžić Maja Ostojić Maja Travar Nijaz Tihić Jasminka Delić Siniša Skočibušić Minela Zekiri-Sivro Antonija Verhaz Dilista Piljić Luka Laura Sanja Duvnjak Maja Zdelar-Tuk Maja Arapović Enisa Šabotić Irena Reil Jadranka Nikolić Sead Ahmetagić Željko Cvetnić Boris Habrun Mile Bosilkovski Silvio Špičić |
| author_sort | Jurica Arapović |
| collection | DOAJ |
| description | ABSTRACT: Objectives: Brucellosis is a ubiquitous emergent bacterial zoonotic disease causing significant human morbidity in Bosnia and Herzegovina. So far, a high rate of resistant Brucella has been found worldwide. This study prospectively analysed the rates of resistance among human Brucella melitensis strains isolated in Bosnia and Herzegovina. Methods: This study included 108 B. melitensis isolates from 209 patients diagnosed at five medical centres in Bosnia and Herzegovina. The resistance profiles of the B. melitensis isolates for the 13 most commonly used antimicrobials were studied in standard Brucella broth (BB) and cation-adjusted Mueller-Hinton broth (CAMHB) supplemented with 4% lysed horse blood or 5% defibrinated sheep blood. Results: Of the 209 patients, B. melitensis blood cultures were positive for 111 (53.1%). Among the 108 isolates investigated, 91 (84.3%) were resistant to trimethoprim-sulfamethoxazole on BB, but not on either CAMHB. Nearly all isolates (>90%) were resistant to azithromycin on BB and both CAMHBs. Conclusion: We observed a high rate of B. melitensis resistance to azithromycin. The high rate of resistance to trimethoprim-sulfamethoxazole that we observed was related to BB, so an alternative broth should be used, such as the enriched CAMHBs in this study, for evaluating resistance to trimethoprim-sulfamethoxazole. Whole-genome sequencing studies are needed to understand the development of antimicrobial resistance in B. melitensis strains isolated from humans. |
| first_indexed | 2024-12-12T09:12:54Z |
| format | Article |
| id | doaj.art-c24c451bca3a4d079321ddd9fa7d9b06 |
| institution | Directory Open Access Journal |
| issn | 2213-7165 |
| language | English |
| last_indexed | 2024-12-12T09:12:54Z |
| publishDate | 2022-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Global Antimicrobial Resistance |
| spelling | doaj.art-c24c451bca3a4d079321ddd9fa7d9b062022-12-22T00:29:28ZengElsevierJournal of Global Antimicrobial Resistance2213-71652022-06-012999104Antimicrobial resistance profiles of human Brucella melitensis isolates in three different microdilution broths: the first multicentre study in Bosnia and HerzegovinaJurica Arapović0Gordan Kompes1Kanita Dedić2Sanela Teskeredžić3Maja Ostojić4Maja Travar5Nijaz Tihić6Jasminka Delić7Siniša Skočibušić8Minela Zekiri-Sivro9Antonija Verhaz10Dilista Piljić11Luka Laura12Sanja Duvnjak13Maja Zdelar-Tuk14Maja Arapović15Enisa Šabotić16Irena Reil17Jadranka Nikolić18Sead Ahmetagić19Željko Cvetnić20Boris Habrun21Mile Bosilkovski22Silvio Špičić23University Clinical Hospital Mostar, Mostar, Bosnia and Herzegovina; School of Medicine, University of Mostar, Mostar, Bosnia and Herzegovina; Corresponding author. University Clinical Hospital Mostar, Kralja tvrtka b.b., Mostar 88000, Bosnia and Herzegovina.Croatian Veterinary Institute, Zagreb, CroatiaCantonal Hospital ‘Dr. Irfan Ljubijankic’, Bihac, Bosnia and HerzegovinaCantonal Hospital Travnik, Travnik, Bosnia and HerzegovinaUniversity Clinical Hospital Mostar, Mostar, Bosnia and HerzegovinaUniversity Clinical Centre of the Republic of Srpska, Banja Luka, The Republic of Srpska, Bosnia and Herzegovina; Faculty of Medicine, University of Banja Luka, Banja Luka, The Republic of Srpska, Bosnia and HerzegovinaUniversity Clinical Center Tuzla, Tuzla, Bosnia and HerzegovinaCantonal Hospital ‘Dr. Irfan Ljubijankic’, Bihac, Bosnia and HerzegovinaUniversity Clinical Hospital Mostar, Mostar, Bosnia and Herzegovina; School of Medicine, University of Mostar, Mostar, Bosnia and HerzegovinaCantonal Hospital Travnik, Travnik, Bosnia and HerzegovinaUniversity Clinical Centre of the Republic of Srpska, Banja Luka, The Republic of Srpska, Bosnia and Herzegovina; Faculty of Medicine, University of Banja Luka, Banja Luka, The Republic of Srpska, Bosnia and HerzegovinaUniversity Clinical Center Tuzla, Tuzla, Bosnia and HerzegovinaVeterinary Institute of Herzegovina–Neretva Canton, Mostar, Bosnia and HerzegovinaCroatian Veterinary Institute, Zagreb, CroatiaCroatian Veterinary Institute, Zagreb, CroatiaSchool of Medicine, University of Mostar, Mostar, Bosnia and Herzegovina; Veterinary Institute of Herzegovina–Neretva Canton, Mostar, Bosnia and HerzegovinaCantonal Hospital ‘Dr. Irfan Ljubijankic’, Bihac, Bosnia and HerzegovinaCroatian Veterinary Institute, Zagreb, CroatiaUniversity Clinical Hospital Mostar, Mostar, Bosnia and Herzegovina; School of Medicine, University of Mostar, Mostar, Bosnia and HerzegovinaUniversity Clinical Center Tuzla, Tuzla, Bosnia and HerzegovinaCroatian Veterinary Institute, Zagreb, CroatiaCroatian Veterinary Institute, Zagreb, CroatiaSs. Cyril and Methodius University in Skopje, Faculty of Medicine, University Clinic for Infectious Diseases, Skopje, Republic of North MacedoniaCroatian Veterinary Institute, Zagreb, Croatia; Corresponding author: Croatian Veterinary Institute, Savska cesta 143, 10000 Zagreb, CroatiaABSTRACT: Objectives: Brucellosis is a ubiquitous emergent bacterial zoonotic disease causing significant human morbidity in Bosnia and Herzegovina. So far, a high rate of resistant Brucella has been found worldwide. This study prospectively analysed the rates of resistance among human Brucella melitensis strains isolated in Bosnia and Herzegovina. Methods: This study included 108 B. melitensis isolates from 209 patients diagnosed at five medical centres in Bosnia and Herzegovina. The resistance profiles of the B. melitensis isolates for the 13 most commonly used antimicrobials were studied in standard Brucella broth (BB) and cation-adjusted Mueller-Hinton broth (CAMHB) supplemented with 4% lysed horse blood or 5% defibrinated sheep blood. Results: Of the 209 patients, B. melitensis blood cultures were positive for 111 (53.1%). Among the 108 isolates investigated, 91 (84.3%) were resistant to trimethoprim-sulfamethoxazole on BB, but not on either CAMHB. Nearly all isolates (>90%) were resistant to azithromycin on BB and both CAMHBs. Conclusion: We observed a high rate of B. melitensis resistance to azithromycin. The high rate of resistance to trimethoprim-sulfamethoxazole that we observed was related to BB, so an alternative broth should be used, such as the enriched CAMHBs in this study, for evaluating resistance to trimethoprim-sulfamethoxazole. Whole-genome sequencing studies are needed to understand the development of antimicrobial resistance in B. melitensis strains isolated from humans.http://www.sciencedirect.com/science/article/pii/S2213716522000406Bosnia and HerzegovinaBrucellosisBrucella melitensisAntimicrobial resistanceMicrobial sensitivity testsAntimicrobial susceptibility breakpoint determination |
| spellingShingle | Jurica Arapović Gordan Kompes Kanita Dedić Sanela Teskeredžić Maja Ostojić Maja Travar Nijaz Tihić Jasminka Delić Siniša Skočibušić Minela Zekiri-Sivro Antonija Verhaz Dilista Piljić Luka Laura Sanja Duvnjak Maja Zdelar-Tuk Maja Arapović Enisa Šabotić Irena Reil Jadranka Nikolić Sead Ahmetagić Željko Cvetnić Boris Habrun Mile Bosilkovski Silvio Špičić Antimicrobial resistance profiles of human Brucella melitensis isolates in three different microdilution broths: the first multicentre study in Bosnia and Herzegovina Journal of Global Antimicrobial Resistance Bosnia and Herzegovina Brucellosis Brucella melitensis Antimicrobial resistance Microbial sensitivity tests Antimicrobial susceptibility breakpoint determination |
| title | Antimicrobial resistance profiles of human Brucella melitensis isolates in three different microdilution broths: the first multicentre study in Bosnia and Herzegovina |
| title_full | Antimicrobial resistance profiles of human Brucella melitensis isolates in three different microdilution broths: the first multicentre study in Bosnia and Herzegovina |
| title_fullStr | Antimicrobial resistance profiles of human Brucella melitensis isolates in three different microdilution broths: the first multicentre study in Bosnia and Herzegovina |
| title_full_unstemmed | Antimicrobial resistance profiles of human Brucella melitensis isolates in three different microdilution broths: the first multicentre study in Bosnia and Herzegovina |
| title_short | Antimicrobial resistance profiles of human Brucella melitensis isolates in three different microdilution broths: the first multicentre study in Bosnia and Herzegovina |
| title_sort | antimicrobial resistance profiles of human brucella melitensis isolates in three different microdilution broths the first multicentre study in bosnia and herzegovina |
| topic | Bosnia and Herzegovina Brucellosis Brucella melitensis Antimicrobial resistance Microbial sensitivity tests Antimicrobial susceptibility breakpoint determination |
| url | http://www.sciencedirect.com/science/article/pii/S2213716522000406 |
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