Effect of Graphene Nanoribbons (TexasPEG) on locomotor function recovery in a rat model of lumbar spinal cord transection
A sharply transected spinal cord has been shown to be fused under the accelerating influence of membrane fusogens such as polyethylene glycol (PEG) (GEMINI protocol). Previous work provided evidence that this is in fact possible. Other fusogens might improve current results. In this study, we aimed...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2018-01-01
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| Series: | Neural Regeneration Research |
| Subjects: | |
| Online Access: | http://www.nrronline.org/article.asp?issn=1673-5374;year=2018;volume=13;issue=8;spage=1440;epage=1446;aulast=Kim |
| _version_ | 1818118053784715264 |
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| author | C-Yoon Kim William K.A. Sikkema Jin Kim Jeong Ah Kim James Walter Raymond Dieter Hyung-Min Chung Andrea Mana James M Tour Sergio Canavero |
| author_facet | C-Yoon Kim William K.A. Sikkema Jin Kim Jeong Ah Kim James Walter Raymond Dieter Hyung-Min Chung Andrea Mana James M Tour Sergio Canavero |
| author_sort | C-Yoon Kim |
| collection | DOAJ |
| description | A sharply transected spinal cord has been shown to be fused under the accelerating influence of membrane fusogens such as polyethylene glycol (PEG) (GEMINI protocol). Previous work provided evidence that this is in fact possible. Other fusogens might improve current results. In this study, we aimed to assess the effects of PEGylated graphene nanoribons (PEG-GNR, and called “TexasPEG” when prepared as 1wt% dispersion in PEG600) versus placebo (saline) on locomotor function recovery and cellular level in a rat model of spinal cord transection at lumbar segment 1 (L1) level. In vivo and in vitro experiments (n = 10 per experiment) were designed. In the in vivo experiment, all rats were submitted to full spinal cord transection at L1 level. Five weeks later, behavioral assessment was performed using the Basso Beattie Bresnahan (BBB) locomotor rating scale. Immunohistochemical staining with neuron marker neurofilament 200 (NF200) antibody and astrocytic scar marker glial fibrillary acidic protein (GFAP) was also performed in the injured spinal cord. In the in vitro experiment, the effects of TexasPEG application for 72 hours on the neurite outgrowth of SH-SY5Y cells were observed under the inverted microscope. Results of both in vivo and in vitro experiments suggest that TexasPEG reduces the formation of glial scars, promotes the regeneration of neurites, and thereby contributes to the recovery of locomotor function of a rat model of spinal cord transfection. |
| first_indexed | 2024-12-11T04:48:12Z |
| format | Article |
| id | doaj.art-c24c5ad4633844b2bd32261a7a427e5d |
| institution | Directory Open Access Journal |
| issn | 1673-5374 |
| language | English |
| last_indexed | 2024-12-11T04:48:12Z |
| publishDate | 2018-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Neural Regeneration Research |
| spelling | doaj.art-c24c5ad4633844b2bd32261a7a427e5d2022-12-22T01:20:28ZengWolters Kluwer Medknow PublicationsNeural Regeneration Research1673-53742018-01-011381440144610.4103/1673-5374.235301Effect of Graphene Nanoribbons (TexasPEG) on locomotor function recovery in a rat model of lumbar spinal cord transectionC-Yoon KimWilliam K.A. SikkemaJin KimJeong Ah KimJames WalterRaymond DieterHyung-Min ChungAndrea ManaJames M TourSergio CanaveroA sharply transected spinal cord has been shown to be fused under the accelerating influence of membrane fusogens such as polyethylene glycol (PEG) (GEMINI protocol). Previous work provided evidence that this is in fact possible. Other fusogens might improve current results. In this study, we aimed to assess the effects of PEGylated graphene nanoribons (PEG-GNR, and called “TexasPEG” when prepared as 1wt% dispersion in PEG600) versus placebo (saline) on locomotor function recovery and cellular level in a rat model of spinal cord transection at lumbar segment 1 (L1) level. In vivo and in vitro experiments (n = 10 per experiment) were designed. In the in vivo experiment, all rats were submitted to full spinal cord transection at L1 level. Five weeks later, behavioral assessment was performed using the Basso Beattie Bresnahan (BBB) locomotor rating scale. Immunohistochemical staining with neuron marker neurofilament 200 (NF200) antibody and astrocytic scar marker glial fibrillary acidic protein (GFAP) was also performed in the injured spinal cord. In the in vitro experiment, the effects of TexasPEG application for 72 hours on the neurite outgrowth of SH-SY5Y cells were observed under the inverted microscope. Results of both in vivo and in vitro experiments suggest that TexasPEG reduces the formation of glial scars, promotes the regeneration of neurites, and thereby contributes to the recovery of locomotor function of a rat model of spinal cord transfection.http://www.nrronline.org/article.asp?issn=1673-5374;year=2018;volume=13;issue=8;spage=1440;epage=1446;aulast=Kimnerve regeneration; spinal cord transfection; spinal cord fusion; GEMINI; TexasPEG; graphene nanoribbons |
| spellingShingle | C-Yoon Kim William K.A. Sikkema Jin Kim Jeong Ah Kim James Walter Raymond Dieter Hyung-Min Chung Andrea Mana James M Tour Sergio Canavero Effect of Graphene Nanoribbons (TexasPEG) on locomotor function recovery in a rat model of lumbar spinal cord transection Neural Regeneration Research nerve regeneration; spinal cord transfection; spinal cord fusion; GEMINI; TexasPEG; graphene nanoribbons |
| title | Effect of Graphene Nanoribbons (TexasPEG) on locomotor function recovery in a rat model of lumbar spinal cord transection |
| title_full | Effect of Graphene Nanoribbons (TexasPEG) on locomotor function recovery in a rat model of lumbar spinal cord transection |
| title_fullStr | Effect of Graphene Nanoribbons (TexasPEG) on locomotor function recovery in a rat model of lumbar spinal cord transection |
| title_full_unstemmed | Effect of Graphene Nanoribbons (TexasPEG) on locomotor function recovery in a rat model of lumbar spinal cord transection |
| title_short | Effect of Graphene Nanoribbons (TexasPEG) on locomotor function recovery in a rat model of lumbar spinal cord transection |
| title_sort | effect of graphene nanoribbons texaspeg on locomotor function recovery in a rat model of lumbar spinal cord transection |
| topic | nerve regeneration; spinal cord transfection; spinal cord fusion; GEMINI; TexasPEG; graphene nanoribbons |
| url | http://www.nrronline.org/article.asp?issn=1673-5374;year=2018;volume=13;issue=8;spage=1440;epage=1446;aulast=Kim |
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