The Osteogenic Potential of Falciform Ligament-Derived Stromal Cells—A Comparative Analysis between Two Osteogenic Induction Programs
Mesenchymal stromal cells (MSCs) have gained special relevance in bone tissue regenerative applications. MSCs have been isolated from different depots, with adipose tissue being acknowledged as one of the most convenient sources, given the wide availability, high cellular yield, and obtainability. R...
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MDPI AG
2022-12-01
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| Series: | Bioengineering |
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| Online Access: | https://www.mdpi.com/2306-5354/9/12/810 |
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| author | Carla Ferreira-Baptista André Queirós Rita Ferreira Maria Helena Fernandes Bruno Colaço Pedro Sousa Gomes |
| author_facet | Carla Ferreira-Baptista André Queirós Rita Ferreira Maria Helena Fernandes Bruno Colaço Pedro Sousa Gomes |
| author_sort | Carla Ferreira-Baptista |
| collection | DOAJ |
| description | Mesenchymal stromal cells (MSCs) have gained special relevance in bone tissue regenerative applications. MSCs have been isolated from different depots, with adipose tissue being acknowledged as one of the most convenient sources, given the wide availability, high cellular yield, and obtainability. Recently, the falciform ligament (FL) has been regarded as a potential depot for adipose tissue-derived stromal cells (FL-ADSCs) isolation. Nonetheless, the osteogenic capability of FL-ADSCs has not been previously characterized. Thus, the present study aimed the detailed characterization of FL-ADSCs’ functionality upon osteogenic induction through a classic (dexamethasone-based-DEX) or an innovative strategy with retinoic acid (RA) in a comparative approach with ADSCs from a control visceral region. Cultures were characterized for cell proliferation, metabolic activity, cellular morphology, fluorescent cytoskeletal and mitochondrial organization, and osteogenic activity–gene expression analysis and cytochemical staining. FL-derived populations expressed significantly higher levels of osteogenic genes and cytochemical markers, particularly with DEX induction, as compared to control ADSCs that were more responsive to RA. FL-ADSCs were identified as a potential source for bone regenerative applications, given the heightened osteogenic functionality. Furthermore, data highlighted the importance of the selection of the most adequate osteogenic-inducing program concerning the specificities of the basal cell population. |
| first_indexed | 2024-03-09T17:19:30Z |
| format | Article |
| id | doaj.art-c24ce54db56f4488878b81b2e2dbf602 |
| institution | Directory Open Access Journal |
| issn | 2306-5354 |
| language | English |
| last_indexed | 2024-03-09T17:19:30Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Bioengineering |
| spelling | doaj.art-c24ce54db56f4488878b81b2e2dbf6022023-11-24T13:21:26ZengMDPI AGBioengineering2306-53542022-12-0191281010.3390/bioengineering9120810The Osteogenic Potential of Falciform Ligament-Derived Stromal Cells—A Comparative Analysis between Two Osteogenic Induction ProgramsCarla Ferreira-Baptista0André Queirós1Rita Ferreira2Maria Helena Fernandes3Bruno Colaço4Pedro Sousa Gomes5Centre for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB), University of Trás-os-Montes and Alto Douro (UTAD), 5000-801 Vila Real, PortugalSCIVET-Grupo Breed, 4580-593 Paredes, PortugalREQUIMTE/LAQV, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, PortugalBoneLab—Laboratory for Bone Metabolism and Regeneration, Faculty of Dental Medicine, University of Porto, 4200-393 Porto, PortugalCentre for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB), University of Trás-os-Montes and Alto Douro (UTAD), 5000-801 Vila Real, PortugalBoneLab—Laboratory for Bone Metabolism and Regeneration, Faculty of Dental Medicine, University of Porto, 4200-393 Porto, PortugalMesenchymal stromal cells (MSCs) have gained special relevance in bone tissue regenerative applications. MSCs have been isolated from different depots, with adipose tissue being acknowledged as one of the most convenient sources, given the wide availability, high cellular yield, and obtainability. Recently, the falciform ligament (FL) has been regarded as a potential depot for adipose tissue-derived stromal cells (FL-ADSCs) isolation. Nonetheless, the osteogenic capability of FL-ADSCs has not been previously characterized. Thus, the present study aimed the detailed characterization of FL-ADSCs’ functionality upon osteogenic induction through a classic (dexamethasone-based-DEX) or an innovative strategy with retinoic acid (RA) in a comparative approach with ADSCs from a control visceral region. Cultures were characterized for cell proliferation, metabolic activity, cellular morphology, fluorescent cytoskeletal and mitochondrial organization, and osteogenic activity–gene expression analysis and cytochemical staining. FL-derived populations expressed significantly higher levels of osteogenic genes and cytochemical markers, particularly with DEX induction, as compared to control ADSCs that were more responsive to RA. FL-ADSCs were identified as a potential source for bone regenerative applications, given the heightened osteogenic functionality. Furthermore, data highlighted the importance of the selection of the most adequate osteogenic-inducing program concerning the specificities of the basal cell population.https://www.mdpi.com/2306-5354/9/12/810mesenchymal stromal cellsadipose tissuefalciform ligamentosteogenesisretinoic acid |
| spellingShingle | Carla Ferreira-Baptista André Queirós Rita Ferreira Maria Helena Fernandes Bruno Colaço Pedro Sousa Gomes The Osteogenic Potential of Falciform Ligament-Derived Stromal Cells—A Comparative Analysis between Two Osteogenic Induction Programs Bioengineering mesenchymal stromal cells adipose tissue falciform ligament osteogenesis retinoic acid |
| title | The Osteogenic Potential of Falciform Ligament-Derived Stromal Cells—A Comparative Analysis between Two Osteogenic Induction Programs |
| title_full | The Osteogenic Potential of Falciform Ligament-Derived Stromal Cells—A Comparative Analysis between Two Osteogenic Induction Programs |
| title_fullStr | The Osteogenic Potential of Falciform Ligament-Derived Stromal Cells—A Comparative Analysis between Two Osteogenic Induction Programs |
| title_full_unstemmed | The Osteogenic Potential of Falciform Ligament-Derived Stromal Cells—A Comparative Analysis between Two Osteogenic Induction Programs |
| title_short | The Osteogenic Potential of Falciform Ligament-Derived Stromal Cells—A Comparative Analysis between Two Osteogenic Induction Programs |
| title_sort | osteogenic potential of falciform ligament derived stromal cells a comparative analysis between two osteogenic induction programs |
| topic | mesenchymal stromal cells adipose tissue falciform ligament osteogenesis retinoic acid |
| url | https://www.mdpi.com/2306-5354/9/12/810 |
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