Rutin ameliorates carbon tetrachloride (CCl4)-induced hepatorenal toxicity and hypogonadism in male rats
Rutin, a food derived-polyphenolic bioflavonoid, has been acknowledged for several health benefits. This study aims to explore the ameliorative effects of rutin against carbon tetrachloride (CCl4) toxicity in male rats. Adult male rats were given either CCl4 (30% in olive oil, 3 ml/kg b.w. intraperi...
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PeerJ Inc.
2019-05-01
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| Online Access: | https://peerj.com/articles/7011.pdf |
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| author | Hany Elsawy Gehan M. Badr Azza Sedky Basem M. Abdallah Abdullah M. Alzahrani Ashraf M. Abdel-Moneim |
| author_facet | Hany Elsawy Gehan M. Badr Azza Sedky Basem M. Abdallah Abdullah M. Alzahrani Ashraf M. Abdel-Moneim |
| author_sort | Hany Elsawy |
| collection | DOAJ |
| description | Rutin, a food derived-polyphenolic bioflavonoid, has been acknowledged for several health benefits. This study aims to explore the ameliorative effects of rutin against carbon tetrachloride (CCl4) toxicity in male rats. Adult male rats were given either CCl4 (30% in olive oil, 3 ml/kg b.w. intraperitoneally) alone or in combination with rutin (70 mg/kg intragastrically) twice a week for 4 weeks. Our data showed that rutin mitigated CCl4 hepatorenal damage, as indicated by diagnostic markers (i.e., transaminases, alkaline phosphatase, total bilirubin, total protein, albumin, urea, uric acid and creatinine), and histopathological findings. In addition, CCl4 induced profound elevation of free radical generation and oxidative stress, as evidenced by increasing lipid peroxidation and reducing catalase, superoxide dismutase and glutathione peroxidase activities in liver, kidney and testicular tissues; these effects were suppressed by coexposure with rutin. Moreover, the increase in the levels of serum triglycerides, cholesterol, low-density lipoprotein cholesterol, and very-low-density lipoprotein cholesterol induced by CCl4 was effectively counteracted by rutin. The decrease in the level of high-density lipoprotein cholesterol in the CCl4 group was also counteracted by rutin treatment. Interestingly, the decreased levels of hormonal mediators associated with sperm production, including serum testosterone, luteinizing hormone and follicle-stimulating hormone, and the impaired sperm quality induced by CCl4 were reversed by rutin. Data from the current study clearly demonstrated that rutin supplementation could at least partly overcome CCl4-induced hepatotoxicity, nephrotoxicity and reproductive toxicity by antioxidant and antidyslipidemic effects. |
| first_indexed | 2024-03-09T07:03:31Z |
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| language | English |
| last_indexed | 2024-03-09T07:03:31Z |
| publishDate | 2019-05-01 |
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| spelling | doaj.art-c24daf86caca483dbcd631fc6b098ed72023-12-03T09:46:30ZengPeerJ Inc.PeerJ2167-83592019-05-017e701110.7717/peerj.7011Rutin ameliorates carbon tetrachloride (CCl4)-induced hepatorenal toxicity and hypogonadism in male ratsHany Elsawy0Gehan M. Badr1Azza Sedky2Basem M. Abdallah3Abdullah M. Alzahrani4Ashraf M. Abdel-Moneim5Department of Chemistry, Faculty of Science, King Faisal University, Al-Hofuf, Al-Ahsa, Saudi ArabiaDepartment of Biological Sciences, Faculty of Science, King Faisal University, Al-Hofuf, Al-Ahsa, Saudi ArabiaDepartment of Biological Sciences, Faculty of Science, King Faisal University, Al-Hofuf, Al-Ahsa, Saudi ArabiaDepartment of Biological Sciences, Faculty of Science, King Faisal University, Al-Hofuf, Al-Ahsa, Saudi ArabiaDepartment of Biological Sciences, Faculty of Science, King Faisal University, Al-Hofuf, Al-Ahsa, Saudi ArabiaDepartment of Biological Sciences, Faculty of Science, King Faisal University, Al-Hofuf, Al-Ahsa, Saudi ArabiaRutin, a food derived-polyphenolic bioflavonoid, has been acknowledged for several health benefits. This study aims to explore the ameliorative effects of rutin against carbon tetrachloride (CCl4) toxicity in male rats. Adult male rats were given either CCl4 (30% in olive oil, 3 ml/kg b.w. intraperitoneally) alone or in combination with rutin (70 mg/kg intragastrically) twice a week for 4 weeks. Our data showed that rutin mitigated CCl4 hepatorenal damage, as indicated by diagnostic markers (i.e., transaminases, alkaline phosphatase, total bilirubin, total protein, albumin, urea, uric acid and creatinine), and histopathological findings. In addition, CCl4 induced profound elevation of free radical generation and oxidative stress, as evidenced by increasing lipid peroxidation and reducing catalase, superoxide dismutase and glutathione peroxidase activities in liver, kidney and testicular tissues; these effects were suppressed by coexposure with rutin. Moreover, the increase in the levels of serum triglycerides, cholesterol, low-density lipoprotein cholesterol, and very-low-density lipoprotein cholesterol induced by CCl4 was effectively counteracted by rutin. The decrease in the level of high-density lipoprotein cholesterol in the CCl4 group was also counteracted by rutin treatment. Interestingly, the decreased levels of hormonal mediators associated with sperm production, including serum testosterone, luteinizing hormone and follicle-stimulating hormone, and the impaired sperm quality induced by CCl4 were reversed by rutin. Data from the current study clearly demonstrated that rutin supplementation could at least partly overcome CCl4-induced hepatotoxicity, nephrotoxicity and reproductive toxicity by antioxidant and antidyslipidemic effects.https://peerj.com/articles/7011.pdfCarbon tetrachlorideHepatorenal failureRutinHypogonadismDyslipidemiaOxidative stress |
| spellingShingle | Hany Elsawy Gehan M. Badr Azza Sedky Basem M. Abdallah Abdullah M. Alzahrani Ashraf M. Abdel-Moneim Rutin ameliorates carbon tetrachloride (CCl4)-induced hepatorenal toxicity and hypogonadism in male rats PeerJ Carbon tetrachloride Hepatorenal failure Rutin Hypogonadism Dyslipidemia Oxidative stress |
| title | Rutin ameliorates carbon tetrachloride (CCl4)-induced hepatorenal toxicity and hypogonadism in male rats |
| title_full | Rutin ameliorates carbon tetrachloride (CCl4)-induced hepatorenal toxicity and hypogonadism in male rats |
| title_fullStr | Rutin ameliorates carbon tetrachloride (CCl4)-induced hepatorenal toxicity and hypogonadism in male rats |
| title_full_unstemmed | Rutin ameliorates carbon tetrachloride (CCl4)-induced hepatorenal toxicity and hypogonadism in male rats |
| title_short | Rutin ameliorates carbon tetrachloride (CCl4)-induced hepatorenal toxicity and hypogonadism in male rats |
| title_sort | rutin ameliorates carbon tetrachloride ccl4 induced hepatorenal toxicity and hypogonadism in male rats |
| topic | Carbon tetrachloride Hepatorenal failure Rutin Hypogonadism Dyslipidemia Oxidative stress |
| url | https://peerj.com/articles/7011.pdf |
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