Haemoglobin Epsilon as a Biomarker for the Molecular Detection of Canine Lymphoma
Lymphoma is a cancer arising from B or T lymphocytes that are central immune system components. It is one of the three most common cancers encountered in the canine; lymphoma affects middle-aged to older dogs and usually stems from lymphatic tissues, such as lymph nodes, lymphoid tissue,...
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| Format: | Article |
| Language: | English |
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University of Baghdad, College of Veterinary Medicine
2023-07-01
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| Series: | The Iraqi Journal of Veterinary Medicine |
| Subjects: | |
| Online Access: | https://jcovm.uobaghdad.edu.iq/index.php/Iraqijvm/article/view/1494 |
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| author | Layla H Al-Kinani Margaret A Sharp Kenneth M Wyatt Flaminia Coiacetto Claire R Sharp Gabriele Rossi Wayne K Greene |
| author_facet | Layla H Al-Kinani Margaret A Sharp Kenneth M Wyatt Flaminia Coiacetto Claire R Sharp Gabriele Rossi Wayne K Greene |
| author_sort | Layla H Al-Kinani |
| collection | DOAJ |
| description |
Lymphoma is a cancer arising from B or T lymphocytes that are central immune system components. It is one of the three most common cancers encountered in the canine; lymphoma affects middle-aged to older dogs and usually stems from lymphatic tissues, such as lymph nodes, lymphoid tissue, or spleen. Despite the advance in the management of canine lymphoma, a better understanding of the subtype and tumor aggressiveness is still crucial for improved clinical diagnosis to differentiate malignancy from hyperplastic conditions and to improve decision-making around treating and what treatment type to use. This study aimed to evaluate a potential novel biomarker related to iron metabolism, embryonic haemoglobin (HBE), for early diagnosis. Archived samples in combination with prospective samples collected from dogs with and without lymphoma were used in this study for the retrospective analyses of this tumor based on the same biomarker amplified by real-time quantitative polymerase chain reaction. The HBE mRNA was aberrantly expressed in canine B and T cell lymphoma compared to the normal lymph node tissue and hyperplastic lymph nodes. In conclusion, this study identified a novel potential biomarker for improving lymphoma diagnosis and treatment in dogs. Further studies with larger sample sizes are needed to confirm the suitability of this biomarker for canine lymphoma diagnosis.
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| first_indexed | 2024-03-13T01:29:25Z |
| format | Article |
| id | doaj.art-c25022fab9fb4a1a8f1f8111695475d1 |
| institution | Directory Open Access Journal |
| issn | 1609-5693 2410-7409 |
| language | English |
| last_indexed | 2024-03-13T01:29:25Z |
| publishDate | 2023-07-01 |
| publisher | University of Baghdad, College of Veterinary Medicine |
| record_format | Article |
| series | The Iraqi Journal of Veterinary Medicine |
| spelling | doaj.art-c25022fab9fb4a1a8f1f8111695475d12023-07-04T09:40:55ZengUniversity of Baghdad, College of Veterinary MedicineThe Iraqi Journal of Veterinary Medicine1609-56932410-74092023-07-0147110.30539/ijvm.v47i1.1494Haemoglobin Epsilon as a Biomarker for the Molecular Detection of Canine LymphomaLayla H Al-Kinani0Margaret A Sharp1Kenneth M Wyatt2Flaminia Coiacetto3Claire R Sharp4Gabriele Rossi5Wayne K Greene6School of Veterinary Medicine, Murdoch University, Perth WA 6150, AustraliaDiscipline of Medical, Molecular and Forensic Sciences, Murdoch University, Perth WA, 6150, AustraliaPerth Veterinary Specialists Centre, Perth WA 6017, AustraliaSchool of Veterinary Medicine, Murdoch University, Perth WA 6150, AustraliaSchool of Veterinary Medicine, Murdoch University, Perth WA 6150School of Veterinary Medicine, Murdoch University, Perth WA 6150Discipline of Medical, Molecular and Forensic Sciences, Murdoch University, Perth WA, 6150, Australia, Lymphoma is a cancer arising from B or T lymphocytes that are central immune system components. It is one of the three most common cancers encountered in the canine; lymphoma affects middle-aged to older dogs and usually stems from lymphatic tissues, such as lymph nodes, lymphoid tissue, or spleen. Despite the advance in the management of canine lymphoma, a better understanding of the subtype and tumor aggressiveness is still crucial for improved clinical diagnosis to differentiate malignancy from hyperplastic conditions and to improve decision-making around treating and what treatment type to use. This study aimed to evaluate a potential novel biomarker related to iron metabolism, embryonic haemoglobin (HBE), for early diagnosis. Archived samples in combination with prospective samples collected from dogs with and without lymphoma were used in this study for the retrospective analyses of this tumor based on the same biomarker amplified by real-time quantitative polymerase chain reaction. The HBE mRNA was aberrantly expressed in canine B and T cell lymphoma compared to the normal lymph node tissue and hyperplastic lymph nodes. In conclusion, this study identified a novel potential biomarker for improving lymphoma diagnosis and treatment in dogs. Further studies with larger sample sizes are needed to confirm the suitability of this biomarker for canine lymphoma diagnosis. https://jcovm.uobaghdad.edu.iq/index.php/Iraqijvm/article/view/1494canine lymphomaembryonic haemoglobinsubunit epsilonRT-qPCR |
| spellingShingle | Layla H Al-Kinani Margaret A Sharp Kenneth M Wyatt Flaminia Coiacetto Claire R Sharp Gabriele Rossi Wayne K Greene Haemoglobin Epsilon as a Biomarker for the Molecular Detection of Canine Lymphoma The Iraqi Journal of Veterinary Medicine canine lymphoma embryonic haemoglobin subunit epsilon RT-qPCR |
| title | Haemoglobin Epsilon as a Biomarker for the Molecular Detection of Canine Lymphoma |
| title_full | Haemoglobin Epsilon as a Biomarker for the Molecular Detection of Canine Lymphoma |
| title_fullStr | Haemoglobin Epsilon as a Biomarker for the Molecular Detection of Canine Lymphoma |
| title_full_unstemmed | Haemoglobin Epsilon as a Biomarker for the Molecular Detection of Canine Lymphoma |
| title_short | Haemoglobin Epsilon as a Biomarker for the Molecular Detection of Canine Lymphoma |
| title_sort | haemoglobin epsilon as a biomarker for the molecular detection of canine lymphoma |
| topic | canine lymphoma embryonic haemoglobin subunit epsilon RT-qPCR |
| url | https://jcovm.uobaghdad.edu.iq/index.php/Iraqijvm/article/view/1494 |
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