Rapid Identification of Antioxidant Compounds of Genista saharae Coss. & Dur. by Combination of DPPH Scavenging Assay and HPTLC-MS
Genista species are sources of antioxidant phenolic compounds such as O- and C-glycosylflavonoids and isoflavonoids. A combination of a DPPH scavenging assay with HPTLC-MS, a fast and efficient method for identification of bioactive compounds, has been applied for evaluation of the radical scavenging...
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MDPI AG
2014-04-01
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| Series: | Molecules |
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| Online Access: | http://www.mdpi.com/1420-3049/19/4/4369 |
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| author | Djamila Meriane Grégory Genta-Jouve Mohamed Kaabeche Sylvie Michel Sabrina Boutefnouchet |
| author_facet | Djamila Meriane Grégory Genta-Jouve Mohamed Kaabeche Sylvie Michel Sabrina Boutefnouchet |
| author_sort | Djamila Meriane |
| collection | DOAJ |
| description | Genista species are sources of antioxidant phenolic compounds such as O- and C-glycosylflavonoids and isoflavonoids. A combination of a DPPH scavenging assay with HPTLC-MS, a fast and efficient method for identification of bioactive compounds, has been applied for evaluation of the radical scavenging activity of metabolites from Genista saharae Coss. & Dur. Different organs collected at various periods have been compared. Identification of antioxidant compounds was obtained by elution of the major DPPH-inhibition zones. The resulting HPTLC-MS analysis under moderately polar conditions, coupled to the DPPH results led to the putative identification of two antioxidant isoflavone aglycones: 3',4',5,7-tetrahydroxyisoflavone (1) and ficuisoflavone (3), whereas polar migration conditions led to the identification of the glycosides 5-methoxy-4',7-trihydroxy-8-glucopyranosylisoflavone (4) and 4',5-dihydroxy-7-methoxyisoflavone-4'-O-β-D-gluco-pyranoside (5). Evaluation of percentage of inhibition of DPPH radical by the purified isoflavone 4 from the root extract showed that it affords a moderate contribution to the total radical scavenging activity of the extract. |
| first_indexed | 2024-12-12T17:05:03Z |
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| id | doaj.art-c2507eac67b84d19a528b36c3e816c01 |
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| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-12T17:05:03Z |
| publishDate | 2014-04-01 |
| publisher | MDPI AG |
| record_format | Article |
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| spelling | doaj.art-c2507eac67b84d19a528b36c3e816c012022-12-22T00:18:01ZengMDPI AGMolecules1420-30492014-04-011944369437910.3390/molecules19044369molecules19044369Rapid Identification of Antioxidant Compounds of Genista saharae Coss. & Dur. by Combination of DPPH Scavenging Assay and HPTLC-MSDjamila Meriane0Grégory Genta-Jouve1Mohamed Kaabeche2Sylvie Michel3Sabrina Boutefnouchet4Laboratoire de Phytothérapie Appliquée aux Maladies Chroniques, Université Ferhat Abbas, Sétif 19000, AlgeriaLaboratoire de Pharmacognosie, U.M.R./C.N.R.S. 8638, Faculté des Sciences Pharmaceutiques et Biologiques, Université Paris Descartes, Sorbonne Paris Cité, 4 Avenue de l'Observatoire, F-75006 Paris, FranceLaboratoire de Phytothérapie Appliquée aux Maladies Chroniques, Université Ferhat Abbas, Sétif 19000, AlgeriaLaboratoire de Pharmacognosie, U.M.R./C.N.R.S. 8638, Faculté des Sciences Pharmaceutiques et Biologiques, Université Paris Descartes, Sorbonne Paris Cité, 4 Avenue de l'Observatoire, F-75006 Paris, FranceLaboratoire de Pharmacognosie, U.M.R./C.N.R.S. 8638, Faculté des Sciences Pharmaceutiques et Biologiques, Université Paris Descartes, Sorbonne Paris Cité, 4 Avenue de l'Observatoire, F-75006 Paris, FranceGenista species are sources of antioxidant phenolic compounds such as O- and C-glycosylflavonoids and isoflavonoids. A combination of a DPPH scavenging assay with HPTLC-MS, a fast and efficient method for identification of bioactive compounds, has been applied for evaluation of the radical scavenging activity of metabolites from Genista saharae Coss. & Dur. Different organs collected at various periods have been compared. Identification of antioxidant compounds was obtained by elution of the major DPPH-inhibition zones. The resulting HPTLC-MS analysis under moderately polar conditions, coupled to the DPPH results led to the putative identification of two antioxidant isoflavone aglycones: 3',4',5,7-tetrahydroxyisoflavone (1) and ficuisoflavone (3), whereas polar migration conditions led to the identification of the glycosides 5-methoxy-4',7-trihydroxy-8-glucopyranosylisoflavone (4) and 4',5-dihydroxy-7-methoxyisoflavone-4'-O-β-D-gluco-pyranoside (5). Evaluation of percentage of inhibition of DPPH radical by the purified isoflavone 4 from the root extract showed that it affords a moderate contribution to the total radical scavenging activity of the extract.http://www.mdpi.com/1420-3049/19/4/4369antioxidantHPTLC-MSGenista saharaeDPPH scavenging assay |
| spellingShingle | Djamila Meriane Grégory Genta-Jouve Mohamed Kaabeche Sylvie Michel Sabrina Boutefnouchet Rapid Identification of Antioxidant Compounds of Genista saharae Coss. & Dur. by Combination of DPPH Scavenging Assay and HPTLC-MS Molecules antioxidant HPTLC-MS Genista saharae DPPH scavenging assay |
| title | Rapid Identification of Antioxidant Compounds of Genista saharae Coss. & Dur. by Combination of DPPH Scavenging Assay and HPTLC-MS |
| title_full | Rapid Identification of Antioxidant Compounds of Genista saharae Coss. & Dur. by Combination of DPPH Scavenging Assay and HPTLC-MS |
| title_fullStr | Rapid Identification of Antioxidant Compounds of Genista saharae Coss. & Dur. by Combination of DPPH Scavenging Assay and HPTLC-MS |
| title_full_unstemmed | Rapid Identification of Antioxidant Compounds of Genista saharae Coss. & Dur. by Combination of DPPH Scavenging Assay and HPTLC-MS |
| title_short | Rapid Identification of Antioxidant Compounds of Genista saharae Coss. & Dur. by Combination of DPPH Scavenging Assay and HPTLC-MS |
| title_sort | rapid identification of antioxidant compounds of genista saharae coss amp dur by combination of dpph scavenging assay and hptlc ms |
| topic | antioxidant HPTLC-MS Genista saharae DPPH scavenging assay |
| url | http://www.mdpi.com/1420-3049/19/4/4369 |
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