Pinobanksin from peony seed husk: A flavonoid with the potential to inhibit the proliferation of SH‐SY5Y
Abstract Pinobanksin, as one of the flavonoids, has powerful biological activities but has been under‐recognized. In this study, we optimized the extraction method of phragmites from peony seed shells by using organic solvent extraction. The yield of PSMS was 10.54 ± 0.13% under the conditions of et...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2024-02-01
|
| Series: | Food Science & Nutrition |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/fsn3.3786 |
| _version_ | 1797306826223517696 |
|---|---|
| author | Wen‐Tao Chen Ying‐Yang Zhang Qiang Qiang Lin‐Ling Zou Ping Zou Ying Xu |
| author_facet | Wen‐Tao Chen Ying‐Yang Zhang Qiang Qiang Lin‐Ling Zou Ping Zou Ying Xu |
| author_sort | Wen‐Tao Chen |
| collection | DOAJ |
| description | Abstract Pinobanksin, as one of the flavonoids, has powerful biological activities but has been under‐recognized. In this study, we optimized the extraction method of phragmites from peony seed shells by using organic solvent extraction. The yield of PSMS was 10.54 ± 0.13% under the conditions of ethanol volume fraction 70%, extraction temperature 70°C, material–liquid ratio 1:25 g/mL, and extraction time 60 min; the optimized PSMS could be effectively separated in S‐8 macroporous resin coupled with C18. The relative content of PSMS was increased from 0.42% in PSMS to 92.53% after C18 purification; the antioxidant activity test revealed that pinobanksin could exert antioxidant ability by binding catalase (CAT) enzyme. Second, it was found that pinobanksin could effectively inhibit the proliferation of SH‐SY5Y cells, mainly by binding to BCL2‐associated X (BAX), B‐cell lymphoma‐2 (BCL‐2), and cyclin‐dependent Kinase 4/6 (CDK4/6) to produce more hydrogen bonds to inhibit their activities. This study confirms the medicinal potential of pinobanksin and provides the basis for the proper understanding of pinobanksin and the development of related products. |
| first_indexed | 2024-03-08T00:47:26Z |
| format | Article |
| id | doaj.art-c25096d815304c9d899b46c033ab5a03 |
| institution | Directory Open Access Journal |
| issn | 2048-7177 |
| language | English |
| last_indexed | 2024-03-08T00:47:26Z |
| publishDate | 2024-02-01 |
| publisher | Wiley |
| record_format | Article |
| series | Food Science & Nutrition |
| spelling | doaj.art-c25096d815304c9d899b46c033ab5a032024-02-15T06:46:47ZengWileyFood Science & Nutrition2048-71772024-02-0112281582910.1002/fsn3.3786Pinobanksin from peony seed husk: A flavonoid with the potential to inhibit the proliferation of SH‐SY5YWen‐Tao Chen0Ying‐Yang Zhang1Qiang Qiang2Lin‐Ling Zou3Ping Zou4Ying Xu5School of Biological and Food Engineering Changzhou University Changzhou Jiangsu ChinaSchool of Biological and Food Engineering Changzhou University Changzhou Jiangsu ChinaChangzhou Wujin No. 3 People's Hospital Changzhou Jiangsu ChinaSchool of Biological and Food Engineering Changzhou University Changzhou Jiangsu ChinaSchool of Biological and Food Engineering Changzhou University Changzhou Jiangsu ChinaSchool of Biological and Food Engineering Changzhou University Changzhou Jiangsu ChinaAbstract Pinobanksin, as one of the flavonoids, has powerful biological activities but has been under‐recognized. In this study, we optimized the extraction method of phragmites from peony seed shells by using organic solvent extraction. The yield of PSMS was 10.54 ± 0.13% under the conditions of ethanol volume fraction 70%, extraction temperature 70°C, material–liquid ratio 1:25 g/mL, and extraction time 60 min; the optimized PSMS could be effectively separated in S‐8 macroporous resin coupled with C18. The relative content of PSMS was increased from 0.42% in PSMS to 92.53% after C18 purification; the antioxidant activity test revealed that pinobanksin could exert antioxidant ability by binding catalase (CAT) enzyme. Second, it was found that pinobanksin could effectively inhibit the proliferation of SH‐SY5Y cells, mainly by binding to BCL2‐associated X (BAX), B‐cell lymphoma‐2 (BCL‐2), and cyclin‐dependent Kinase 4/6 (CDK4/6) to produce more hydrogen bonds to inhibit their activities. This study confirms the medicinal potential of pinobanksin and provides the basis for the proper understanding of pinobanksin and the development of related products.https://doi.org/10.1002/fsn3.3786C18 isolationmolecular dynamicspharmacophore screeningpodophyllotoxin |
| spellingShingle | Wen‐Tao Chen Ying‐Yang Zhang Qiang Qiang Lin‐Ling Zou Ping Zou Ying Xu Pinobanksin from peony seed husk: A flavonoid with the potential to inhibit the proliferation of SH‐SY5Y Food Science & Nutrition C18 isolation molecular dynamics pharmacophore screening podophyllotoxin |
| title | Pinobanksin from peony seed husk: A flavonoid with the potential to inhibit the proliferation of SH‐SY5Y |
| title_full | Pinobanksin from peony seed husk: A flavonoid with the potential to inhibit the proliferation of SH‐SY5Y |
| title_fullStr | Pinobanksin from peony seed husk: A flavonoid with the potential to inhibit the proliferation of SH‐SY5Y |
| title_full_unstemmed | Pinobanksin from peony seed husk: A flavonoid with the potential to inhibit the proliferation of SH‐SY5Y |
| title_short | Pinobanksin from peony seed husk: A flavonoid with the potential to inhibit the proliferation of SH‐SY5Y |
| title_sort | pinobanksin from peony seed husk a flavonoid with the potential to inhibit the proliferation of sh sy5y |
| topic | C18 isolation molecular dynamics pharmacophore screening podophyllotoxin |
| url | https://doi.org/10.1002/fsn3.3786 |
| work_keys_str_mv | AT wentaochen pinobanksinfrompeonyseedhuskaflavonoidwiththepotentialtoinhibittheproliferationofshsy5y AT yingyangzhang pinobanksinfrompeonyseedhuskaflavonoidwiththepotentialtoinhibittheproliferationofshsy5y AT qiangqiang pinobanksinfrompeonyseedhuskaflavonoidwiththepotentialtoinhibittheproliferationofshsy5y AT linlingzou pinobanksinfrompeonyseedhuskaflavonoidwiththepotentialtoinhibittheproliferationofshsy5y AT pingzou pinobanksinfrompeonyseedhuskaflavonoidwiththepotentialtoinhibittheproliferationofshsy5y AT yingxu pinobanksinfrompeonyseedhuskaflavonoidwiththepotentialtoinhibittheproliferationofshsy5y |