Transcriptional Expression of the <i>ompA</i>, <i>cpaf</i>, <i>tarp</i>, and <i>tox</i> Genes of <i>Chlamydia trachomatis</i> Clinical Isolates at Different Stages of the Developmental Cycle
The transcriptional gene expression patterns of <i>Chlamydia trachomatis</i> have mainly been studied using reference strains propagated in cultured cells. Here, using five low-passage-number <i>C. trachomatis</i> clinical isolates that originated from asymptomatic or symptom...
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MDPI AG
2019-05-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/7/6/153 |
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| author | Suvi Korhonen Kati Hokynar Laura Mannonen Jorma Paavonen Eija Hiltunen-Back Mirja Puolakkainen |
| author_facet | Suvi Korhonen Kati Hokynar Laura Mannonen Jorma Paavonen Eija Hiltunen-Back Mirja Puolakkainen |
| author_sort | Suvi Korhonen |
| collection | DOAJ |
| description | The transcriptional gene expression patterns of <i>Chlamydia trachomatis</i> have mainly been studied using reference strains propagated in cultured cells. Here, using five low-passage-number <i>C. trachomatis</i> clinical isolates that originated from asymptomatic or symptomatic female patients, the in vitro expression of the <i>ompA</i>, <i>cpaf</i>, <i>tarp</i>, and <i>tox</i> genes was studied with reverse transcriptase real-time PCR during the chlamydial developmental cycle. We observed dissimilarities in the gene expression patterns between the low-passage-number clinical isolates and the reference strains. The expression of <i>ompA</i> and the peak of the <i>tox</i> expression were observed earlier in the reference strains than in most of the clinical isolates. The expression of <i>cpaf</i> was high in the reference strains compared with the clinical isolates at the mid-phase (6−24 hours post infection) of the developmental cycle. All of the strains had a rather similar <i>tarp</i> expression profile. Four out of five clinical isolates exhibited slower growth kinetics compared with the reference strains. The use of low-passage-number <i>C. trachomatis</i> clinical isolates instead of reference strains in the studies might better reflect the situation in human infection. |
| first_indexed | 2024-04-13T13:26:30Z |
| format | Article |
| id | doaj.art-c250e87a50454bf195e8bd0226b80269 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-04-13T13:26:30Z |
| publishDate | 2019-05-01 |
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| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-c250e87a50454bf195e8bd0226b802692022-12-22T02:45:08ZengMDPI AGMicroorganisms2076-26072019-05-017615310.3390/microorganisms7060153microorganisms7060153Transcriptional Expression of the <i>ompA</i>, <i>cpaf</i>, <i>tarp</i>, and <i>tox</i> Genes of <i>Chlamydia trachomatis</i> Clinical Isolates at Different Stages of the Developmental CycleSuvi Korhonen0Kati Hokynar1Laura Mannonen2Jorma Paavonen3Eija Hiltunen-Back4Mirja Puolakkainen5Department of Virology, University of Helsinki and Helsinki University Hospital, FI-00014 Helsinki, FinlandDepartment of Virology, University of Helsinki and Helsinki University Hospital, FI-00014 Helsinki, FinlandDepartment of Virology, University of Helsinki and Helsinki University Hospital, FI-00014 Helsinki, FinlandDepartment of Obstetrics and Gynecology, University of Helsinki and Helsinki University Hospital, FI-00029 HUS Helsinki, FinlandClinic of Venereal Diseases, Skin and Allergy Hospital, Helsinki University Hospital, FI-00029 HUS Helsinki, FinlandDepartment of Virology, University of Helsinki and Helsinki University Hospital, FI-00014 Helsinki, FinlandThe transcriptional gene expression patterns of <i>Chlamydia trachomatis</i> have mainly been studied using reference strains propagated in cultured cells. Here, using five low-passage-number <i>C. trachomatis</i> clinical isolates that originated from asymptomatic or symptomatic female patients, the in vitro expression of the <i>ompA</i>, <i>cpaf</i>, <i>tarp</i>, and <i>tox</i> genes was studied with reverse transcriptase real-time PCR during the chlamydial developmental cycle. We observed dissimilarities in the gene expression patterns between the low-passage-number clinical isolates and the reference strains. The expression of <i>ompA</i> and the peak of the <i>tox</i> expression were observed earlier in the reference strains than in most of the clinical isolates. The expression of <i>cpaf</i> was high in the reference strains compared with the clinical isolates at the mid-phase (6−24 hours post infection) of the developmental cycle. All of the strains had a rather similar <i>tarp</i> expression profile. Four out of five clinical isolates exhibited slower growth kinetics compared with the reference strains. The use of low-passage-number <i>C. trachomatis</i> clinical isolates instead of reference strains in the studies might better reflect the situation in human infection.https://www.mdpi.com/2076-2607/7/6/153<i>Chlamydia trachomatis</i>transcriptional expressionlow-passage-number isolates |
| spellingShingle | Suvi Korhonen Kati Hokynar Laura Mannonen Jorma Paavonen Eija Hiltunen-Back Mirja Puolakkainen Transcriptional Expression of the <i>ompA</i>, <i>cpaf</i>, <i>tarp</i>, and <i>tox</i> Genes of <i>Chlamydia trachomatis</i> Clinical Isolates at Different Stages of the Developmental Cycle Microorganisms <i>Chlamydia trachomatis</i> transcriptional expression low-passage-number isolates |
| title | Transcriptional Expression of the <i>ompA</i>, <i>cpaf</i>, <i>tarp</i>, and <i>tox</i> Genes of <i>Chlamydia trachomatis</i> Clinical Isolates at Different Stages of the Developmental Cycle |
| title_full | Transcriptional Expression of the <i>ompA</i>, <i>cpaf</i>, <i>tarp</i>, and <i>tox</i> Genes of <i>Chlamydia trachomatis</i> Clinical Isolates at Different Stages of the Developmental Cycle |
| title_fullStr | Transcriptional Expression of the <i>ompA</i>, <i>cpaf</i>, <i>tarp</i>, and <i>tox</i> Genes of <i>Chlamydia trachomatis</i> Clinical Isolates at Different Stages of the Developmental Cycle |
| title_full_unstemmed | Transcriptional Expression of the <i>ompA</i>, <i>cpaf</i>, <i>tarp</i>, and <i>tox</i> Genes of <i>Chlamydia trachomatis</i> Clinical Isolates at Different Stages of the Developmental Cycle |
| title_short | Transcriptional Expression of the <i>ompA</i>, <i>cpaf</i>, <i>tarp</i>, and <i>tox</i> Genes of <i>Chlamydia trachomatis</i> Clinical Isolates at Different Stages of the Developmental Cycle |
| title_sort | transcriptional expression of the i ompa i i cpaf i i tarp i and i tox i genes of i chlamydia trachomatis i clinical isolates at different stages of the developmental cycle |
| topic | <i>Chlamydia trachomatis</i> transcriptional expression low-passage-number isolates |
| url | https://www.mdpi.com/2076-2607/7/6/153 |
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