Development of engineered Candida tropicalis strain for efficient corncob-based xylitol-ethanol biorefinery
Abstract Background Xylitol has a wide range of applications in the pharmaceuticals, cosmetic, food and beverage industry. Microbial xylitol production reduces the risk of contamination and is considered as environment friendly and sustainable compared to the chemical method. In this study, random m...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2023-10-01
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| Series: | Microbial Cell Factories |
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| Online Access: | https://doi.org/10.1186/s12934-023-02190-3 |
| _version_ | 1797451005785276416 |
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| author | Anup Kumar Singh Farha Deeba Mohit Kumar Sonam Kumari Shahid Ali Wani Tanushree Paul Naseem A. Gaur |
| author_facet | Anup Kumar Singh Farha Deeba Mohit Kumar Sonam Kumari Shahid Ali Wani Tanushree Paul Naseem A. Gaur |
| author_sort | Anup Kumar Singh |
| collection | DOAJ |
| description | Abstract Background Xylitol has a wide range of applications in the pharmaceuticals, cosmetic, food and beverage industry. Microbial xylitol production reduces the risk of contamination and is considered as environment friendly and sustainable compared to the chemical method. In this study, random mutagenesis and genetic engineering approaches were employed to develop Candida tropicalis strains with reduced xylitol dehydrogenase (XDH) activity to eliminate co-substrate requirement for corn cob-based xylitol-ethanol biorefinery. Results The results suggest that when pure xylose (10% w/v) was fermented in bioreactor, the Ethyl methane sulfonate (EMS) mutated strain (C. tropicalis K2M) showed 9.2% and XYL2 heterozygous (XYL2/xyl2Δ::FRT) strain (C. tropicalis K21D) showed 16% improvement in xylitol production compared to parental strain (C. tropicalis K2). Furthermore, 1.5-fold improvement (88.62 g/L to 132 g/L) in xylitol production was achieved by C. tropicalis K21D after Response Surface Methodology (RSM) and one factor at a time (OFAT) applied for media component optimization. Finally, corncob hydrolysate was tested for xylitol production in biorefinery mode, which leads to the production of 32.6 g/L xylitol from hemicellulosic fraction, 32.0 g/L ethanol from cellulosic fraction and 13.0 g/L animal feed. Conclusions This work, for the first time, illustrates the potential of C. tropicalis K21D as a microbial cell factory for efficient production of xylitol and ethanol via an integrated biorefinery framework by utilising lignocellulosic biomass with minimum waste generation. |
| first_indexed | 2024-03-09T14:48:41Z |
| format | Article |
| id | doaj.art-c2546d1d7cae4874b73242da2fe0b01a |
| institution | Directory Open Access Journal |
| issn | 1475-2859 |
| language | English |
| last_indexed | 2024-03-09T14:48:41Z |
| publishDate | 2023-10-01 |
| publisher | BMC |
| record_format | Article |
| series | Microbial Cell Factories |
| spelling | doaj.art-c2546d1d7cae4874b73242da2fe0b01a2023-11-26T14:38:04ZengBMCMicrobial Cell Factories1475-28592023-10-0122111610.1186/s12934-023-02190-3Development of engineered Candida tropicalis strain for efficient corncob-based xylitol-ethanol biorefineryAnup Kumar Singh0Farha Deeba1Mohit Kumar2Sonam Kumari3Shahid Ali Wani4Tanushree Paul5Naseem A. Gaur6Yeast Biofuel Group, DBT-ICGEB Centre for Advanced Bioenergy Research, International Centre for Genetic Engineering and Biotechnology (ICGEB)Yeast Biofuel Group, DBT-ICGEB Centre for Advanced Bioenergy Research, International Centre for Genetic Engineering and Biotechnology (ICGEB)Yeast Biofuel Group, DBT-ICGEB Centre for Advanced Bioenergy Research, International Centre for Genetic Engineering and Biotechnology (ICGEB)Yeast Biofuel Group, DBT-ICGEB Centre for Advanced Bioenergy Research, International Centre for Genetic Engineering and Biotechnology (ICGEB)Yeast Biofuel Group, DBT-ICGEB Centre for Advanced Bioenergy Research, International Centre for Genetic Engineering and Biotechnology (ICGEB)Yeast Biofuel Group, DBT-ICGEB Centre for Advanced Bioenergy Research, International Centre for Genetic Engineering and Biotechnology (ICGEB)Yeast Biofuel Group, DBT-ICGEB Centre for Advanced Bioenergy Research, International Centre for Genetic Engineering and Biotechnology (ICGEB)Abstract Background Xylitol has a wide range of applications in the pharmaceuticals, cosmetic, food and beverage industry. Microbial xylitol production reduces the risk of contamination and is considered as environment friendly and sustainable compared to the chemical method. In this study, random mutagenesis and genetic engineering approaches were employed to develop Candida tropicalis strains with reduced xylitol dehydrogenase (XDH) activity to eliminate co-substrate requirement for corn cob-based xylitol-ethanol biorefinery. Results The results suggest that when pure xylose (10% w/v) was fermented in bioreactor, the Ethyl methane sulfonate (EMS) mutated strain (C. tropicalis K2M) showed 9.2% and XYL2 heterozygous (XYL2/xyl2Δ::FRT) strain (C. tropicalis K21D) showed 16% improvement in xylitol production compared to parental strain (C. tropicalis K2). Furthermore, 1.5-fold improvement (88.62 g/L to 132 g/L) in xylitol production was achieved by C. tropicalis K21D after Response Surface Methodology (RSM) and one factor at a time (OFAT) applied for media component optimization. Finally, corncob hydrolysate was tested for xylitol production in biorefinery mode, which leads to the production of 32.6 g/L xylitol from hemicellulosic fraction, 32.0 g/L ethanol from cellulosic fraction and 13.0 g/L animal feed. Conclusions This work, for the first time, illustrates the potential of C. tropicalis K21D as a microbial cell factory for efficient production of xylitol and ethanol via an integrated biorefinery framework by utilising lignocellulosic biomass with minimum waste generation.https://doi.org/10.1186/s12934-023-02190-3Strain developmentXylitolC. tropicalisCorncobXDH |
| spellingShingle | Anup Kumar Singh Farha Deeba Mohit Kumar Sonam Kumari Shahid Ali Wani Tanushree Paul Naseem A. Gaur Development of engineered Candida tropicalis strain for efficient corncob-based xylitol-ethanol biorefinery Microbial Cell Factories Strain development Xylitol C. tropicalis Corncob XDH |
| title | Development of engineered Candida tropicalis strain for efficient corncob-based xylitol-ethanol biorefinery |
| title_full | Development of engineered Candida tropicalis strain for efficient corncob-based xylitol-ethanol biorefinery |
| title_fullStr | Development of engineered Candida tropicalis strain for efficient corncob-based xylitol-ethanol biorefinery |
| title_full_unstemmed | Development of engineered Candida tropicalis strain for efficient corncob-based xylitol-ethanol biorefinery |
| title_short | Development of engineered Candida tropicalis strain for efficient corncob-based xylitol-ethanol biorefinery |
| title_sort | development of engineered candida tropicalis strain for efficient corncob based xylitol ethanol biorefinery |
| topic | Strain development Xylitol C. tropicalis Corncob XDH |
| url | https://doi.org/10.1186/s12934-023-02190-3 |
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