Optimizing early infant diagnosis at delivery rooms with HIV-1 Abbott RealTime-PCR using phosphate buffered saline to complement low plasma volumes
Background: Improving the early identification of HIV-1-infected newborns with birth testing is critical to comprehensive early infant diagnosis and care for newborns living with HIV-1. Automated RNA quantification systems are valuable diagnostic tools, but the volume of plasma that viral load platf...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-11-01
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| Series: | Journal of Clinical Virology Plus |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2667038022000527 |
| _version_ | 1811187607018668032 |
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| author | Jaqueline Helena da Silva Santos Andressa Coelho Sichi Cintia Mayumi Ahagon Norberto Camilo Campos Luís Fernando de Macedo Brígido |
| author_facet | Jaqueline Helena da Silva Santos Andressa Coelho Sichi Cintia Mayumi Ahagon Norberto Camilo Campos Luís Fernando de Macedo Brígido |
| author_sort | Jaqueline Helena da Silva Santos |
| collection | DOAJ |
| description | Background: Improving the early identification of HIV-1-infected newborns with birth testing is critical to comprehensive early infant diagnosis and care for newborns living with HIV-1. Automated RNA quantification systems are valuable diagnostic tools, but the volume of plasma that viral load platforms require makes their widespread use for young children difficult. Method: Seventy-nine plasma samples with different viral load ranges were evaluated in parallel with the use of 1x PBS, pH 7.4, to supplement the required volume at dilutions factors from 1:2 to 1:50. Viral load quantification assays were evaluated using ABBOTT Molecular platforms, USA. Results: Using 1x PBS, at 1:10 dilution (70 µL plasma in 630 µL 1x PBS), a sensitivity of 100% and 100% specificity were obtained for detecting a viremia above 400 copies/mL (Kappa of 0.96, p < 0, 0001) for 1:50 dilution the sensitivity was 96% and the specificity 100% (kappa 0.90, p < 0.0001). Conclusions: Although with reduced sensitivity, proportional to the dilution factor, the use of plasma does not influence the specificity of the test and allows the diagnosis of HIV-1 infections. Cases with very low viremia, a situation that may occur due to the treatment or prophylaxis of the mother and/or child, may go unnoticed with this procedure, and undiluted testing may be necessary. |
| first_indexed | 2024-04-11T14:06:14Z |
| format | Article |
| id | doaj.art-c254c4c8b0984e54be5b9ba93bc298e7 |
| institution | Directory Open Access Journal |
| issn | 2667-0380 |
| language | English |
| last_indexed | 2024-04-11T14:06:14Z |
| publishDate | 2022-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Clinical Virology Plus |
| spelling | doaj.art-c254c4c8b0984e54be5b9ba93bc298e72022-12-22T04:19:53ZengElsevierJournal of Clinical Virology Plus2667-03802022-11-0124100113Optimizing early infant diagnosis at delivery rooms with HIV-1 Abbott RealTime-PCR using phosphate buffered saline to complement low plasma volumesJaqueline Helena da Silva Santos0Andressa Coelho Sichi1Cintia Mayumi Ahagon2Norberto Camilo Campos3Luís Fernando de Macedo Brígido4Virology Center, Adolfo Lutz Institute, São Paulo, São Paulo, BrazilVirology Center, Adolfo Lutz Institute, São Paulo, São Paulo, BrazilVirology Center, Adolfo Lutz Institute, São Paulo, São Paulo, BrazilVirology Center, Adolfo Lutz Institute, São Paulo, São Paulo, BrazilCorresponding author.; Virology Center, Adolfo Lutz Institute, São Paulo, São Paulo, BrazilBackground: Improving the early identification of HIV-1-infected newborns with birth testing is critical to comprehensive early infant diagnosis and care for newborns living with HIV-1. Automated RNA quantification systems are valuable diagnostic tools, but the volume of plasma that viral load platforms require makes their widespread use for young children difficult. Method: Seventy-nine plasma samples with different viral load ranges were evaluated in parallel with the use of 1x PBS, pH 7.4, to supplement the required volume at dilutions factors from 1:2 to 1:50. Viral load quantification assays were evaluated using ABBOTT Molecular platforms, USA. Results: Using 1x PBS, at 1:10 dilution (70 µL plasma in 630 µL 1x PBS), a sensitivity of 100% and 100% specificity were obtained for detecting a viremia above 400 copies/mL (Kappa of 0.96, p < 0, 0001) for 1:50 dilution the sensitivity was 96% and the specificity 100% (kappa 0.90, p < 0.0001). Conclusions: Although with reduced sensitivity, proportional to the dilution factor, the use of plasma does not influence the specificity of the test and allows the diagnosis of HIV-1 infections. Cases with very low viremia, a situation that may occur due to the treatment or prophylaxis of the mother and/or child, may go unnoticed with this procedure, and undiluted testing may be necessary.http://www.sciencedirect.com/science/article/pii/S2667038022000527Early infant diagnosisNeonatalViral load, HIV-1Real-time PCRPhosphate buffered saline |
| spellingShingle | Jaqueline Helena da Silva Santos Andressa Coelho Sichi Cintia Mayumi Ahagon Norberto Camilo Campos Luís Fernando de Macedo Brígido Optimizing early infant diagnosis at delivery rooms with HIV-1 Abbott RealTime-PCR using phosphate buffered saline to complement low plasma volumes Journal of Clinical Virology Plus Early infant diagnosis Neonatal Viral load, HIV-1 Real-time PCR Phosphate buffered saline |
| title | Optimizing early infant diagnosis at delivery rooms with HIV-1 Abbott RealTime-PCR using phosphate buffered saline to complement low plasma volumes |
| title_full | Optimizing early infant diagnosis at delivery rooms with HIV-1 Abbott RealTime-PCR using phosphate buffered saline to complement low plasma volumes |
| title_fullStr | Optimizing early infant diagnosis at delivery rooms with HIV-1 Abbott RealTime-PCR using phosphate buffered saline to complement low plasma volumes |
| title_full_unstemmed | Optimizing early infant diagnosis at delivery rooms with HIV-1 Abbott RealTime-PCR using phosphate buffered saline to complement low plasma volumes |
| title_short | Optimizing early infant diagnosis at delivery rooms with HIV-1 Abbott RealTime-PCR using phosphate buffered saline to complement low plasma volumes |
| title_sort | optimizing early infant diagnosis at delivery rooms with hiv 1 abbott realtime pcr using phosphate buffered saline to complement low plasma volumes |
| topic | Early infant diagnosis Neonatal Viral load, HIV-1 Real-time PCR Phosphate buffered saline |
| url | http://www.sciencedirect.com/science/article/pii/S2667038022000527 |
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