The cell-type-specific spatial organization of the anterior thalamic nuclei of the mouse brain
Summary: Understanding the cell-type composition and spatial organization of brain regions is crucial for interpreting brain computation and function. In the thalamus, the anterior thalamic nuclei (ATN) are involved in a wide variety of functions, yet the cell-type composition of the ATN remains unm...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2024-03-01
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| Series: | Cell Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124724001700 |
| _version_ | 1797287655566737408 |
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| author | Margarita Kapustina Angela A. Zhang Jennifer Y.J. Tsai Brianna N. Bristow Larissa Kraus Kaitlin E. Sullivan Sarah R. Erwin Lihua Wang Tara R. Stach Jody Clements Andrew L. Lemire Mark S. Cembrowski |
| author_facet | Margarita Kapustina Angela A. Zhang Jennifer Y.J. Tsai Brianna N. Bristow Larissa Kraus Kaitlin E. Sullivan Sarah R. Erwin Lihua Wang Tara R. Stach Jody Clements Andrew L. Lemire Mark S. Cembrowski |
| author_sort | Margarita Kapustina |
| collection | DOAJ |
| description | Summary: Understanding the cell-type composition and spatial organization of brain regions is crucial for interpreting brain computation and function. In the thalamus, the anterior thalamic nuclei (ATN) are involved in a wide variety of functions, yet the cell-type composition of the ATN remains unmapped at a single-cell and spatial resolution. Combining single-cell RNA sequencing, spatial transcriptomics, and multiplexed fluorescent in situ hybridization, we identify three discrete excitatory cell-type clusters that correspond to the known nuclei of the ATN and uncover marker genes, molecular pathways, and putative functions of these cell types. We further illustrate graded spatial variation along the dorsomedial-ventrolateral axis for all individual nuclei of the ATN and additionally demonstrate that the anteroventral nucleus exhibits spatially covarying protein products and long-range inputs. Collectively, our study reveals discrete and continuous cell-type organizational principles of the ATN, which will help to guide and interpret experiments on ATN computation and function. |
| first_indexed | 2024-03-07T18:36:32Z |
| format | Article |
| id | doaj.art-c257c515fb4c47c9915ea9bd0ab33363 |
| institution | Directory Open Access Journal |
| issn | 2211-1247 |
| language | English |
| last_indexed | 2024-03-07T18:36:32Z |
| publishDate | 2024-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj.art-c257c515fb4c47c9915ea9bd0ab333632024-03-02T04:53:59ZengElsevierCell Reports2211-12472024-03-01433113842The cell-type-specific spatial organization of the anterior thalamic nuclei of the mouse brainMargarita Kapustina0Angela A. Zhang1Jennifer Y.J. Tsai2Brianna N. Bristow3Larissa Kraus4Kaitlin E. Sullivan5Sarah R. Erwin6Lihua Wang7Tara R. Stach8Jody Clements9Andrew L. Lemire10Mark S. Cembrowski11Department of Cellular and Physiological Sciences, Life Sciences Institute, University of British Columbia, 2350 Health Sciences Mall, Vancouver, BC V6T 1Z3, CanadaDepartment of Cellular and Physiological Sciences, Life Sciences Institute, University of British Columbia, 2350 Health Sciences Mall, Vancouver, BC V6T 1Z3, CanadaDepartment of Cellular and Physiological Sciences, Life Sciences Institute, University of British Columbia, 2350 Health Sciences Mall, Vancouver, BC V6T 1Z3, CanadaDepartment of Cellular and Physiological Sciences, Life Sciences Institute, University of British Columbia, 2350 Health Sciences Mall, Vancouver, BC V6T 1Z3, CanadaDepartment of Cellular and Physiological Sciences, Life Sciences Institute, University of British Columbia, 2350 Health Sciences Mall, Vancouver, BC V6T 1Z3, CanadaDepartment of Cellular and Physiological Sciences, Life Sciences Institute, University of British Columbia, 2350 Health Sciences Mall, Vancouver, BC V6T 1Z3, CanadaDepartment of Cellular and Physiological Sciences, Life Sciences Institute, University of British Columbia, 2350 Health Sciences Mall, Vancouver, BC V6T 1Z3, CanadaJanelia Research Campus, HHMI, 19700 Helix Drive, Ashburn, VA 20147, USASchool of Biomedical Engineering, Biomedical Research Centre, University of British Columbia, 2222 Health Sciences Mall, Vancouver, BC V6T 1Z3, CanadaJanelia Research Campus, HHMI, 19700 Helix Drive, Ashburn, VA 20147, USAJanelia Research Campus, HHMI, 19700 Helix Drive, Ashburn, VA 20147, USADepartment of Cellular and Physiological Sciences, Life Sciences Institute, University of British Columbia, 2350 Health Sciences Mall, Vancouver, BC V6T 1Z3, Canada; Djavad Mowafaghian Centre for Brain Health, University of British Columbia, 2215 Wesbrook Mall, Vancouver, BC V6T 1Z3, Canada; Janelia Research Campus, HHMI, 19700 Helix Drive, Ashburn, VA 20147, USA; Corresponding authorSummary: Understanding the cell-type composition and spatial organization of brain regions is crucial for interpreting brain computation and function. In the thalamus, the anterior thalamic nuclei (ATN) are involved in a wide variety of functions, yet the cell-type composition of the ATN remains unmapped at a single-cell and spatial resolution. Combining single-cell RNA sequencing, spatial transcriptomics, and multiplexed fluorescent in situ hybridization, we identify three discrete excitatory cell-type clusters that correspond to the known nuclei of the ATN and uncover marker genes, molecular pathways, and putative functions of these cell types. We further illustrate graded spatial variation along the dorsomedial-ventrolateral axis for all individual nuclei of the ATN and additionally demonstrate that the anteroventral nucleus exhibits spatially covarying protein products and long-range inputs. Collectively, our study reveals discrete and continuous cell-type organizational principles of the ATN, which will help to guide and interpret experiments on ATN computation and function.http://www.sciencedirect.com/science/article/pii/S2211124724001700CP: NeuroscienceCP: Cell biology |
| spellingShingle | Margarita Kapustina Angela A. Zhang Jennifer Y.J. Tsai Brianna N. Bristow Larissa Kraus Kaitlin E. Sullivan Sarah R. Erwin Lihua Wang Tara R. Stach Jody Clements Andrew L. Lemire Mark S. Cembrowski The cell-type-specific spatial organization of the anterior thalamic nuclei of the mouse brain Cell Reports CP: Neuroscience CP: Cell biology |
| title | The cell-type-specific spatial organization of the anterior thalamic nuclei of the mouse brain |
| title_full | The cell-type-specific spatial organization of the anterior thalamic nuclei of the mouse brain |
| title_fullStr | The cell-type-specific spatial organization of the anterior thalamic nuclei of the mouse brain |
| title_full_unstemmed | The cell-type-specific spatial organization of the anterior thalamic nuclei of the mouse brain |
| title_short | The cell-type-specific spatial organization of the anterior thalamic nuclei of the mouse brain |
| title_sort | cell type specific spatial organization of the anterior thalamic nuclei of the mouse brain |
| topic | CP: Neuroscience CP: Cell biology |
| url | http://www.sciencedirect.com/science/article/pii/S2211124724001700 |
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