Single step multiple genotyping by MALDI-TOF mass spectrometry, for evaluation of minor histocompatibility antigens in patients submitted to allogeneic stem cell transplantation from HLA-matched related and unrelated donor
The outcome of patients underwent to allogeneic stem cell transplantation (allo- SCT) is closely related to graft versus host disease (GvHD) and graft versus leukemia (GvL) effects which can be mediated by mHAgs. 23 mHAgs have been identified and reported to be differently correlated with GVHD or GV...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2017-09-01
|
| Series: | Hematology Reports |
| Subjects: | |
| Online Access: | http://www.pagepress.org/journals/index.php/hr/article/view/7051 |
| _version_ | 1797935138794897408 |
|---|---|
| author | Federica Cattina Simona Bernardi Vilma Mantovani Eleonora Toffoletti Alessandra Santoro Domenico Pastore Bruno Martino Giuseppe Console Giovanni Martinelli Michele Malagola |
| author_facet | Federica Cattina Simona Bernardi Vilma Mantovani Eleonora Toffoletti Alessandra Santoro Domenico Pastore Bruno Martino Giuseppe Console Giovanni Martinelli Michele Malagola |
| author_sort | Federica Cattina |
| collection | DOAJ |
| description | The outcome of patients underwent to allogeneic stem cell transplantation (allo- SCT) is closely related to graft versus host disease (GvHD) and graft versus leukemia (GvL) effects which can be mediated by mHAgs. 23 mHAgs have been identified and reported to be differently correlated with GVHD or GVL and the aim of this work was develop a method to genotype the mHAgs described so far. For this study we used MALDI-TOF iPLEX Gold Mass Array technology. We tested 46 donor/recipient matched pairs that underwent allo-SCT because of Philadelphia positive (Ph+) chronic myeloid leukemia (n=29) or Ph+ acute lymphoblastic leukemia (n=17). Our data show that sibling pairs had a lesser number of mHAgs mismatches compared to MUD pairs. Notably, donor/recipient genomic mismatch on DPH1 was correlated with an increased risk of acute GvHD and LB-ADIR-1R mismatch on graft versus host direction was correlated with a better RFS with no increase of GvHD risk. Our work provides a simple, accurate and highly automatable method for mHAgs genotyping and suggest the role of mHAgs in addressing the immune reaction between donor and host. |
| first_indexed | 2024-04-10T18:09:41Z |
| format | Article |
| id | doaj.art-c259055060e647bd89f8cd28bfe141d1 |
| institution | Directory Open Access Journal |
| issn | 2038-8322 2038-8330 |
| language | English |
| last_indexed | 2024-04-10T18:09:41Z |
| publishDate | 2017-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Hematology Reports |
| spelling | doaj.art-c259055060e647bd89f8cd28bfe141d12023-02-02T11:36:52ZengMDPI AGHematology Reports2038-83222038-83302017-09-019310.4081/hr.2017.70513781Single step multiple genotyping by MALDI-TOF mass spectrometry, for evaluation of minor histocompatibility antigens in patients submitted to allogeneic stem cell transplantation from HLA-matched related and unrelated donorFederica Cattina0Simona Bernardi1Vilma Mantovani2Eleonora Toffoletti3Alessandra Santoro4Domenico Pastore5Bruno Martino6Giuseppe Console7Giovanni Martinelli8Michele Malagola9Unit of Blood Disease and Stem Cell Transplantation, Department of Clinical and Experimental Sciences, University of Brescia, ASST Spedali Civili of BresciaUnit of Blood Disease and Stem Cell Transplantation, Department of Clinical and Experimental Sciences, University of Brescia, ASST Spedali Civili of BresciaCenter for Applied Biomedical Research (CRBA), St. Orsola-Malpighi University Hospital, BolognaDivision of Hematology and Bone Marrow Transplantation, Department of Experimental and Clinical Medical Sciences, Azienda Ospedaliero-Universitaria di UdineDivision of Hematology and Bone Marrow Transplantation, Ospedali Riuniti Villa Sofia-Cervello, PalermoHematology and Bone Marrow Transplantation Unit, Department of Emergency and Organ Transplantation, University of BariHematology Division, AO BMM, Reggio CalabriaHematology and Bone Marrow Transplant Unit, AO BMM, Reggio CalabriaDepartment of Experimental, Diagnostic and Specialty Medicine, Hematology “L.eA. Seràgnoli”, University of BolognaUnit of Blood Disease and Stem Cell Transplantation, Department of Clinical and Experimental Sciences, University of Brescia, ASST Spedali Civili of BresciaThe outcome of patients underwent to allogeneic stem cell transplantation (allo- SCT) is closely related to graft versus host disease (GvHD) and graft versus leukemia (GvL) effects which can be mediated by mHAgs. 23 mHAgs have been identified and reported to be differently correlated with GVHD or GVL and the aim of this work was develop a method to genotype the mHAgs described so far. For this study we used MALDI-TOF iPLEX Gold Mass Array technology. We tested 46 donor/recipient matched pairs that underwent allo-SCT because of Philadelphia positive (Ph+) chronic myeloid leukemia (n=29) or Ph+ acute lymphoblastic leukemia (n=17). Our data show that sibling pairs had a lesser number of mHAgs mismatches compared to MUD pairs. Notably, donor/recipient genomic mismatch on DPH1 was correlated with an increased risk of acute GvHD and LB-ADIR-1R mismatch on graft versus host direction was correlated with a better RFS with no increase of GvHD risk. Our work provides a simple, accurate and highly automatable method for mHAgs genotyping and suggest the role of mHAgs in addressing the immune reaction between donor and host.http://www.pagepress.org/journals/index.php/hr/article/view/7051Stem cell transplantation, HLA, minor Histocompatibility antigens |
| spellingShingle | Federica Cattina Simona Bernardi Vilma Mantovani Eleonora Toffoletti Alessandra Santoro Domenico Pastore Bruno Martino Giuseppe Console Giovanni Martinelli Michele Malagola Single step multiple genotyping by MALDI-TOF mass spectrometry, for evaluation of minor histocompatibility antigens in patients submitted to allogeneic stem cell transplantation from HLA-matched related and unrelated donor Hematology Reports Stem cell transplantation, HLA, minor Histocompatibility antigens |
| title | Single step multiple genotyping by MALDI-TOF mass spectrometry, for evaluation of minor histocompatibility antigens in patients submitted to allogeneic stem cell transplantation from HLA-matched related and unrelated donor |
| title_full | Single step multiple genotyping by MALDI-TOF mass spectrometry, for evaluation of minor histocompatibility antigens in patients submitted to allogeneic stem cell transplantation from HLA-matched related and unrelated donor |
| title_fullStr | Single step multiple genotyping by MALDI-TOF mass spectrometry, for evaluation of minor histocompatibility antigens in patients submitted to allogeneic stem cell transplantation from HLA-matched related and unrelated donor |
| title_full_unstemmed | Single step multiple genotyping by MALDI-TOF mass spectrometry, for evaluation of minor histocompatibility antigens in patients submitted to allogeneic stem cell transplantation from HLA-matched related and unrelated donor |
| title_short | Single step multiple genotyping by MALDI-TOF mass spectrometry, for evaluation of minor histocompatibility antigens in patients submitted to allogeneic stem cell transplantation from HLA-matched related and unrelated donor |
| title_sort | single step multiple genotyping by maldi tof mass spectrometry for evaluation of minor histocompatibility antigens in patients submitted to allogeneic stem cell transplantation from hla matched related and unrelated donor |
| topic | Stem cell transplantation, HLA, minor Histocompatibility antigens |
| url | http://www.pagepress.org/journals/index.php/hr/article/view/7051 |
| work_keys_str_mv | AT federicacattina singlestepmultiplegenotypingbymalditofmassspectrometryforevaluationofminorhistocompatibilityantigensinpatientssubmittedtoallogeneicstemcelltransplantationfromhlamatchedrelatedandunrelateddonor AT simonabernardi singlestepmultiplegenotypingbymalditofmassspectrometryforevaluationofminorhistocompatibilityantigensinpatientssubmittedtoallogeneicstemcelltransplantationfromhlamatchedrelatedandunrelateddonor AT vilmamantovani singlestepmultiplegenotypingbymalditofmassspectrometryforevaluationofminorhistocompatibilityantigensinpatientssubmittedtoallogeneicstemcelltransplantationfromhlamatchedrelatedandunrelateddonor AT eleonoratoffoletti singlestepmultiplegenotypingbymalditofmassspectrometryforevaluationofminorhistocompatibilityantigensinpatientssubmittedtoallogeneicstemcelltransplantationfromhlamatchedrelatedandunrelateddonor AT alessandrasantoro singlestepmultiplegenotypingbymalditofmassspectrometryforevaluationofminorhistocompatibilityantigensinpatientssubmittedtoallogeneicstemcelltransplantationfromhlamatchedrelatedandunrelateddonor AT domenicopastore singlestepmultiplegenotypingbymalditofmassspectrometryforevaluationofminorhistocompatibilityantigensinpatientssubmittedtoallogeneicstemcelltransplantationfromhlamatchedrelatedandunrelateddonor AT brunomartino singlestepmultiplegenotypingbymalditofmassspectrometryforevaluationofminorhistocompatibilityantigensinpatientssubmittedtoallogeneicstemcelltransplantationfromhlamatchedrelatedandunrelateddonor AT giuseppeconsole singlestepmultiplegenotypingbymalditofmassspectrometryforevaluationofminorhistocompatibilityantigensinpatientssubmittedtoallogeneicstemcelltransplantationfromhlamatchedrelatedandunrelateddonor AT giovannimartinelli singlestepmultiplegenotypingbymalditofmassspectrometryforevaluationofminorhistocompatibilityantigensinpatientssubmittedtoallogeneicstemcelltransplantationfromhlamatchedrelatedandunrelateddonor AT michelemalagola singlestepmultiplegenotypingbymalditofmassspectrometryforevaluationofminorhistocompatibilityantigensinpatientssubmittedtoallogeneicstemcelltransplantationfromhlamatchedrelatedandunrelateddonor |